Mustard Oil in “<i>Shibori Daikon</i>” a Variety of Japanese Radish, Selectively Inhibits the Proliferation of H-<i>ras</i>-Transformed 3Y1 Cells

Yamasaki, Masao; Omi, Yusuke; Fujii, Nobutaka; Ozaki, Asako; Nakama, Akihiko; Sakakibara, Yoichi; Suiko, Masahito; Nishiyama, Kazuo

doi:10.1271/bbb.90322

Cited by 15 publications

(14 citation statements)

References 34 publications

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“…(2,3) For instance, the HR-3Y1-2 cell line, derived from 3Y1 rat fibroblasts transformed with the v-H- ras oncogene, (4) show anchorage-independent proliferation and changes in cellular morphology, (5) and HR-3Y1-2 cells are capable of proliferating faster than the parental cell line 3Y1. (6) Therefore, a component that can suppress of activated Ras can be promising agent for alleviation of malignant characters of cancer cells. In our previous study in which we explored the components that was capable of suppressing the proliferation of HR-3Y1-2 cells, α-lipoic acid (LA) was shown to selectively inhibit the proliferation of HR-3Y1-2 cells.…”

Section: Introductionmentioning

confidence: 99%

α-Lipoic acid suppresses migration and invasion via downregulation of cell surface β1-integrin expression in bladder cancer cells

Yamasaki

Iwase

Kawano

et al. 2014

J. Clin. Biochem. Nutr.

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Our previous study showed α-lipoic acid (LA) downregulated cell surface β1-integrin expression of v-H-ras-transformed derivative of rat fibroblast with amelioration of their malignant phenotype. Here, we evaluated the ameliorating effect of LA on the malignant characters in H-ras-transformed bladder cancer cells. H-ras mutated bladder cancer line, T24 cells were incubated with LA to evaluate the inhibitory effect on proliferation, migration, invasion and β1-integrin expression. Fluorescence staining of F-actin and western blotting analyses of the related signaling pathways were also performed. LA inhibited the proliferation of T24 cells. Cell adhesion to collagen IV and fibronectin was strikingly inhibited by LA treatment accompanied by downregulation of cell surface but not whole cell β1-integrin expression. LA clearly inhibited cell migration and invasion of T24 cells, which were mimicked by extracellular signal-regulated kinase (ERK) and Akt pathway inhibition. Actually, LA significantly downregulated the phosphorylated ERK and Akt levels. Moreover, LA downregulated phosphorylated focal adhesion kinase level with disappearance of stress fiber formation. Finally, although LA induced the internalization of cell surface β1-integrin, disruption of the raft did not affect the action of LA. Taken together, LA is a promising agent to improve malignant character of bladder cancer cells through regulation of cellular β1-integrin localization.

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Section: Introductionmentioning

confidence: 99%

α-Lipoic acid suppresses migration and invasion via downregulation of cell surface β1-integrin expression in bladder cancer cells

Yamasaki

Iwase

Kawano

et al. 2014

J. Clin. Biochem. Nutr.

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“…Recently, a number of studies have demonstrated that radishes or radish extracts have biological activity including antioxidant (Lugasi and others 2005; Wang and others 2010), antimutagenic (Nakamura and others 2001), and antiproliferative effects (Papi and others 2008; Yamasaki and others 2009; Beevi and others 2010) as well as induction of detoxification enzymes (Lee and Lee 2006; Hanlon and others 2007). These studies used aqueous (Ghayur and Gilani 2005; Lugasi and others 2005; Hanlon and others 2009), methanolic (Takaya and others 2003; Salah‐Abbes and others 2009), and hydrophobic (Yamasaki and others 2009; Beevi and others 2010) radish extracts or specific phytochemicals that are present in radishes including glucosinolates and isothiocyanates (Visentin and others 1992; Nakamura and others 2001; Barillari and others 2005; Hanlon and others 2007; Papi and others 2008; Ben Salah‐Abbes and others 2009). In addition, studies have shown that radishes contain other phytochemicals that have been associated with beneficial health effects including phenolic acids (Sgherri and others 2003) and anthocyanins (Otsuki and others 2002; Liu and others 2008; Wang and others 2010).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Composition and Biological Activity of 8 Varieties of Radish (Raphanus sativus L.) Sprouts and Mature Taproots

Hanlon¹,

Barnes²

2011

Journal of Food Science

112

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Radishes (Raphanus sativus L.) are members of the cruciferous vegetable family that contain many classes of biologically active phytochemicals. This study determined the phytochemical composition of the sprouts and mature taproots of 8 radish varieties. Radish sprouts contained significantly greater concentrations of glucosinolates (3.8-fold) and isothiocyanates (8.2-fold) than the mature radish taproot and also contained significantly greater concentrations of phenolics (on average 6.9-fold). The anthocyanin concentrations of the mature radish taproot were significantly greater than in the sprouts of red, pink, and purple varieties. The primary anthocyanidins present in the red and pink radish varieties were pelargonidin and delphinidin, while the primary anthocyanidin in the purple radish variety was cyanidin. Radish sprouts were between 9- and 59-fold more potent than the corresponding mature taproot at activating the antioxidant response element (ARE) in a stably transfected hepatoma cell line. The ARE activity of the radish sprouts and mature taproots was significantly correlated with the total isothiocyanate concentration of the radishes. Practical Application: Understanding the influence variety and developmental stage has on the biological activity of cruciferous vegetables provides important information for further studies examining the in vivo effects of radish treatment and foundation for providing recommendations to reduce the risk of chronic disease through dietary intervention.

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“…Since the–Cys group was predicted to enter into the HDAC pocket, other isothiocyanates that are metabolized via the mercapturic pathway were considered candidate HDAC inhibitors, including those found in pungent foods such as mustard, radish, horseradish, wasabi, and daikon (Higdon et al, 2007; Nian et al, 2009a; Verkerk et al, 2009; Yamasaki et al, 2009; Ernst et al, 2010). The mustard oil compound allyl isothiocya-nate was reported earlier to induce histone acetylation in mouse erythroleukemia cells, but with no apparent inhibition of HDAC activity (Lea et al, 2001).…”

Section: Dietary Hdac Inhibitors—role Of Metabolismmentioning

confidence: 99%

Metabolism as a key to histone deacetylase inhibition

Rajendran

Williams

et al. 2011

Critical Reviews in Biochemistry and Molecular Biology

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There is growing interest in the epigenetic mechanisms that are dysregulated in cancer and other human pathologies. Under this broad umbrella, modulators of histone deacetylase (HDAC) activity have gained interest as both cancer chemopreventive and therapeutic agents. Of the first generation, FDA-approved HDAC inhibitors to have progressed to clinical trials, vorinostat represents a “direct acting” compound with structural features suitable for docking into the HDAC pocket, whereas romidepsin can be considered a prodrug that undergoes reductive metabolism to generate the active intermediate (a zinc-binding thiol). It is now evident that other agents, including those in the human diet, can be converted by metabolism to intermediates that affect HDAC activity. Examples are cited of short-chain fatty acids, seleno-α-keto acids, small molecule thiols, mercapturic acid metabolites, indoles, and polyphenols. The findings are discussed in the context of putative endogenous HDAC inhibitors generated by intermediary metabolism (e.g. pyruvate), the yin–yang of HDAC inhibition versus HDAC activation, and the screening assays that might be most appropriate for discovery of novel HDAC inhibitors in the future.

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Mustard Oil in “Shibori Daikon” a Variety of Japanese Radish, Selectively Inhibits the Proliferation of H-ras-Transformed 3Y1 Cells

Cited by 15 publications

References 34 publications

α-Lipoic acid suppresses migration and invasion via downregulation of cell surface β1-integrin expression in bladder cancer cells

α-Lipoic acid suppresses migration and invasion via downregulation of cell surface β1-integrin expression in bladder cancer cells

Phytochemical Composition and Biological Activity of 8 Varieties of Radish (Raphanus sativus L.) Sprouts and Mature Taproots

Metabolism as a key to histone deacetylase inhibition

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