<b>Influence of seasonality and production method on the antibacterial activity of propolis</b>

Abstract

ABSTRACT. The antibacterial activity of propolis produced throughout the year by different methods of collection ('intelligent' collector of propolis -ICP; plastic screen -PC; conventional scraping -CS) on Staphylococcos aureus and Escherichia coli is investigated. Fifteen beehives (five per collector) of Africanized Apis mellifera were used. Monthly produced propolis, with the same collection technique, was mixed for the preparation of the extract. The ethanol extract of propolis (EEP) was prepared at the ratio of 30% (30 g of propolis, completing the volume for 100 mL with ethanol 70%). Two microorganisms, a positive bacterium Gram Staphylococcus aureus and a negative bacterium Gram Escherichia coli, through the methodology of diffusion in agar, were used for the biological activity evaluation of EEP. Results show that propolis presented antibacterial activity, affected by seasonality and by collecting method.Keywords: apiculture, biological properties, quality, bacteria.Influência da sazonalidade e método de produção na atividade antibacteriana da própolis RESUMO. O objetivo deste estudo foi investigar a atividade antibacteriana da própolis produzida ao longo do ano por diferentes métodos de coleta (coletor de própolis 'inteligente' -CPI, tela plástica -TP e raspagem convencional -RC) sobre Staphylococcos aureus e Escherichia coli. Foram utilizadas 15 colmeias de Apis mellifera africanizadas. Para o preparo do extrato, foi misturada a própolis produzida mensalmente pela mesma técnica de coleta. O EAP foi preparado na proporção de 30% (30 g de própolis completando o volume para 100 mL com etanol 70%). Para avaliação da atividade biológica do EAP, foram usados dois micro-organismos: uma bactéria gram positivia Staphylococcus aureus e uma gram negativa Escherichia coli, por meio da metodologia de difusão em ágar. Os resultados mostraram que a própolis apresenta atividade antibacteriana, sendo esta influenciada pela sazonalidade e método de colheita utilizado.Palavras-chave: apicultura, atividade biológica, qualidade, bactéria.

Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics

“…Numerous studies have demonstrated that propolis possess a marked antibacterial, antiviral, and moderate antifungal activity [ 21 , 22 , 23 ]. The results presented by Seidel et al [ 24 ] documented that propolis of North American, South American and European origins had MIC ranging from 0.125 to >0.5 mg/mL, while samples of African and Asian origin had MIC ranging from 0.08 to >0.5 mg/mL.…”

Effects of seasonal variations and collection methods on the mineral composition of propolis from Apis mellifera Linnaeus Beehives

“…The regional chemical composition of propolis can interfere in its biological properties (Simões-Ambrosio et al, 2010;Toreti et al, 2013;Bankova et al, 2014, Souza et al, 2014. The presence of phenolic compounds, steroids, terpenes, and amino acids in propolis has been extensively studied (Sawaya et al, 2011;Finger et al, 2014).…”

Maintenance of quality of eggs submitted to treatment with propolis extract and sanitizers

“…The efficiency of the antimicrobial activity of the propolis extract of Brazilian origin is proven against Gram positive and Gram negative microorganisms of animal origin (Gomes, Ítavo, Leal, Ítavo, & Lunas, 2016;Klahr et al, 2019;Souza, Inoue, Fernandes Júnior, Veiga, & Orsi, 2014). Among the components associated with this property, phenolic compounds and flavonoids stand out, which possibly damage the cellular structure of these microorganisms, inhibiting their growth or even eliminating them (Przybylek & Karpinski, 2019).…”