&lt;em&gt;Candida albicans&lt;/em&gt; susceptibility to lactoperoxidase-generated hypoiodite

Courtois, Philippe

doi:10.2147/ccide.s10891

Cited by 18 publications

(2 citation statements)

References 11 publications

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“…For example, Ihalin and group successively reported that the addition of SCN − into the peroxidase/H 2 O 2 /I − system abolished the bactericidal activity of the oxidized halide (Ihalin et al, 1998, 2001). Similarly, Ahariz and Courtois (2010) demonstrated a competition between I − and SCN − , reporting that the addition of SCN − to peroxidase/G/GOD/I − system (G/GOD: glucose/glucose oxidase as source of hydrogen peroxide) decreased the antifungal effect of the system.…”

Section: Discussionmentioning

confidence: 89%

Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems

et al. 2017

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Antibiotic resistance is an increasingly serious threat to global health. Consequently, the development of non-antibiotic based therapies and disinfectants, which avoid induction of resistance, or cross-resistance, is of high priority. We report the synthesis of a biocidal complex, which is produced by the reaction between ionic oxidizable salts—iodide and thiocyanate—in the presence of hydrogen peroxide as an oxidation source. The reaction generates bactericidal reactive oxygen and iodine species. In this study, we report that the iodo-thiocyanate complex (ITC) is an effective bactericidal agent with activity against planktonic and biofilm cells of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and methicillin-resistant S. aureus) bacteria. The minimum bactericidal concentrations and the minimum biofilm eradication concentrations of the biocidal composite were in the range of 7.8–31.3 and 31.3–250 μg ml−1, respectively. As a result, the complex was capable to cause a rapid cell death of planktonic test cultures at between 0.5 and 2 h, and complete eradication of dual and mono-species biofilms between 30 s and 10 min. Furthermore, the test bacteria, including a MRSA strain, exposed to the cocktail failed to develop resistance after serial passages. The antimicrobial activity of the ITC appears to derive from the combinational effect of the powerful species capable of oxidizing the essential biomolecules of bacteria. The use of this composition may provide an effective and efficient method for killing potential pathogens, as well as for disinfecting and removing biofilm contamination.

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Section: Discussionmentioning

confidence: 89%

Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems

et al. 2017

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“…LPS was found to kill some catalase-positive bacteria, such as Gram-negative pseudomonas, salmonellae, shigellae, and coliforms bacteria in the proper environments, including specific temperature and pH of the medium, bacterial inoculum, and incubation period 6,9 . While LPS was found to inhibit but does not kill catalase-negative bacteria, such as Gram-positive streptococci and lactobacilli 10,11 . Moreover, LPS can significantly delay the growth of numerous psychrotrophic bacteria, such as several strains of the Micrococcus, Bacillus, and Pseudomonas genera 12–15 .…”

Section: Introductionmentioning

confidence: 98%

Preparation and characterization of novel nanocombination of bovine lactoperoxidase with Dye Decolorizing and anti-bacterial activity

El‐Fakharany

Abd-Elhamid

El‐Deeb

2019

Sci Rep

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Interaction between nanoparticles (NPs) and protein is particularly important due to the formation of dynamic nanoparticle-protein complex. The current study indicated that silica NPs were able to induce conformational modification in the adsorbed lactoperoxidase (LPO) which in turns degrades the synthetic dyes. The maximum degradation efficiency was recorded for the LPO modified silica NPs in the presence of H 2 O 2 comparing with either free LPO or silica NPs. Degradation efficiency of crystal violet and commassie blue R250 after 6 h was assessed to be 100(%). Also, degradation efficiency of Congo red reached 90.6% and 79.3% in the presence and absence of H 2 O 2 , respectively, however methyl red degradation efficiency recorded 85%. The viability assay experiment indicated that the IC 50 value of the LPO modified silica NPs on human fibroblast cells reached 2.8 mg/ml after 48 h incubation. In addition to dye removal, the LPO modified silica NPs were able to inhibit the antibiotic resistant bacterial strains ( Salmonell typhii , Staphylococcus areus , Pseudomonas aureginosa , E . coli , Proteus sp . and streptococcus sp .) at concentrations up to 2.5 mg/ml with inhibition activity about 95%. These findings emphasized that the ability of LPO for degradation of the synthetic dyes after adsorption on silica NPs besides it could be a promising agent with potent inhibitory effect targeting a wide range of multidrug resistant bacteria.

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Reaction Product Variability and Biological Activity of the Lactoperoxidase System Depending on Medium Ionic Strength and pH, and on Substrate Relative Concentration

Bafort

Damblon

Smargiasso

et al. 2018

Chemistry & Biodiversity

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The potential of ions produced in water by the lactoperoxidase system against plant pests has shown promising results. We tested the bioactivity of ions produced by the lactoperoxidase oxidation of I and SCN in several buffers or in tap water and characterized the ions produced. In vitro biological activity was tested against Penicillium expansum, the causal agent of mold in fruits, and the major cause of patulin contamination of fruit juices and compotes. In buffers, the ionic concentration was increased 3-fold, and pathogen inhibition was obtained down to the 1:15 dilution. In tap water, the ionic concentration was weaker, and pathogen inhibition was obtained only down to the 1:3 dilution. Acidic buffer increased ion concentrations as compared to less acidic (pH 5.6 or 6.2) or neutral buffers, as do increased ionic strength. C-labelled SCN and MS showed that different ions were produced in water and in buffers. In specific conditions the ion solution turned yellow and a product was formed, probably diiodothiocyanate (I SCN ), giving an intense signal at 49.7 ppm in C-NMR. The formation of the signal was unambiguously favored in acidic media and disadvantaged or inhibited in neutral or basic conditions. It was enhanced at a specific SCN : I ratio of 1:4.5, but decreased when the ratio was 1:2, and was inhibited at ratio SCN >I . We demonstrated that the formation of the signal required the interaction between I and SCN , and MS showed the presence of I SCN .

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<em>Candida albicans</em> susceptibility to lactoperoxidase-generated hypoiodite

Cited by 18 publications

References 11 publications

Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems

Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems

Preparation and characterization of novel nanocombination of bovine lactoperoxidase with Dye Decolorizing and anti-bacterial activity

Reaction Product Variability and Biological Activity of the Lactoperoxidase System Depending on Medium Ionic Strength and pH, and on Substrate Relative Concentration

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