&lt;em&gt;In vivo&lt;/em&gt; antimalarial activity of the ethanolic leaf extract of &lt;em&gt;Hyptis suaveolens&lt;/em&gt; poit on &lt;em&gt;Plasmodium berghei&lt;/em&gt; in Mice

Dawet, A.; Anyanwu, IG; Dede, MP; Nr, Uzoigwe; ONyekwelu, NA

doi:10.4314/ijbcs.v6i1.11

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…It has been discovered that several plants have antimalarial properties (Ngbolua et al, 2011;Oluyemi and Samuel, 2017;Willcox et al, 2004). Extracts from bark of Cinchona officinalis and leaves of Artemisia annua are prototypical for the development of antimalarial drugs from natural products; while some plants have also demonstrated high level of antimalarial activities (Dawet et al, 2012;Adzu et al, 2008). However, resistance to currently available antimalarial agents has been reported (Wongsrichanalai and Meshnick, 2008).…”

Section: Introductionmentioning

confidence: 99%

Isolation and structure elucidation of anti-malarial principles from Terminalia mantaly H. Perrier stem bark

Samuel¹,

Adekunle²

2021

Int. J. Bio. Chem. Sci

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Emergence of malaria parasite resistance to drugs has raised global public health concerns for a compelling need to develop improved malaria therapy. This study is a bio-guided isolation of triterpenoid antimalarial compounds from Terminalia mantaly. Methanol extract of the plant was subjected to column chromatography, and eluted with a ternary solvent system gradient-wise. Two compounds, 1 and 2, were isolated and characterised by spectroscopic data (IR, 1H and 13C NMR, COSY, HMQC, HMBC) and by comparison with literature. Isolated compounds were investigated for antimalarial property by spectrophotometric determination of inhibition of β-Hematin formation, absorbance taken at 405 nm. Results were analysed using Graghpad Prism® (6.0) and presented as mean IC50±SEM. Statistical significance, determined using Student’s t-test and one-way ANOVA, set at p-value of 0.05. Quantitative β-Hematin formation inhibitory activities gave IC50±SEM values of (compound 1; 4.434±0.47), (compound 2; 5.140±4.2) with (chloroquine; 0.335±0.1 mg/ml). Compound 1 was identified as 2,3,19,23-tetrahydroxyolean-12-en-28-oic acid glucopyranoside (arjunglucoside I), and compound2 as its aglycone, 2,3,19,23-tetrahydroxyolean-12-en-28-oic acid (arjungenin). This study provided credence for folkloric use of Terminalia mantaly to treat malaria, and this observed activity was probably due to these isolated triterpenoids.Keywords: β-Hematin, triterpenoids, nuclear magnetic resonance spectroscopy

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Section: Introductionmentioning

confidence: 99%

Isolation and structure elucidation of anti-malarial principles from Terminalia mantaly H. Perrier stem bark

Samuel¹,

Adekunle²

2021

Int. J. Bio. Chem. Sci

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“…The artesunate control was comparably highly active with IC 50 of 0.002 µg/mL. H. suaveolens has been reported elsewhere to show good antiplasmodial activity both in-vitro and in-vivo 32,33 . Ethanol and hydroethanol leaf extracts of H. suaveolens showed good antiplasmodial activity with an IC 50 < 6.25 µg/mL on the chloroquine-resistant (Dd2) strain of P. falciparum 34 .…”

Section: Suppressive Effects Of the Extracts On Parasitementioning

confidence: 73%

Untitled

2023

IJPSR

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A number of the population in developing countries use herbs and herbal products to treat various diseases including malaria. However, most of them have not been evaluated for their efficacy and toxicity and their use is not standardized in some instances. Poly-herbal extracts may yield better treatment outcomes compared to extracts from a single plant due to the combined effects of bioactive compounds from the multiple sources. Cassia obtusifolia and Hyptis suaveolens are two plants commonly used in herbal preparations for treating malaria in Ghana. This study was to investigate Plasmodium falciparum asexual growth inhibitory activities of the individual and combined leaf extracts of the two plants. Leaves of the C. obtusifolia and H. suaveolens were extracted separately with methanol, ethanol and water. The six extracts, as well as in combination were evaluated in a dose-dependent manner for their antiplasmodial activities against the chloroquine-sensitive Plasmodium falciparum 3D7. Aqueous extract of C. obtusifolia, including the mixture of the two plants were highly active with mean IC 50 less than 4.0 µg/mL. Similarly, methanol extract of H. suaveolens and the mixture also had IC 50 below 4 µg/mL. Ethanol extracts of the mixture was correspondingly active with IC 50 less than 4.0 µg/ml. All the combined extracts inhibited parasite growth above 80% at concentration of 100.00 µg/mL. Our results showed good antiplasmodial activity of the two plants comparable to the standard drug, artesunate, and hence provides the basis for their continuous use as antimalarial therapy in Ghana, possibly in their combined form.

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“…[43] It inhibits growth of both chloroquine sensitive and chloroquine-resistant strains of Plasmodium falciparum under in vitro conditions. diterpenoid 13alpha-epi-dioxiabiet-8( 14)-en-18-ol set up from petroleum ether excerpt of H. suaveolens leaves also shows antiplasmodial exertion [44,45] .…”

Section: Anti Plasmodic Acitivitymentioning

confidence: 98%

The Phytochemical and Pharmacological Screening of Hyptis Suaveolens

Dhamale¹,

Manikpuriya²,

Sanap³

et al. 2023

World J. Adv. Eng. Technol. Sci.

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The main aim of this article is to know the importance of traditional plant i.e Hyptis Suaveolens. It belong to family Lamiaceace, commonly called as Wialaitis tulsi and it grow on sub-tropic and semi- arid environment. It use broadly and belong to Subkingdom – Tracheobionta, Super kingdom – Spermatophyte , Division – Magnoliophyte, Class – Magnoliopsida, Subclass – Asteridae, Order – Lamiales. Hyptis is widespread in Australia its perennial, annual ,herb or sub-herb. The leaves of Hyptis is opposite and ovate about 2.5 to 10cm in length. The flower is dark purple in color and auxiliary with long stalk, hairy calyx and about 4mm long. The seeds are flat and mucilaginous fruit (nut let) are about 1.2-1.5mm long cure swellings, abscesses and hemorrhoids. In India the plant is considered to be stimulant, \carminative, soporifics and lactogogue.Commonly it is called as Horehound, Pignut, Wild spikenard, Gross Baume, Hyptis odder (French), Alfavaca-brava, Betônica-brava (Portuguese, Brazil), Chao, Hierba de las mules, hortela do campo (Spanish), Wilayati tulsi (Hindi), bhustrena, darp tulas, jungli tulas (Marathi), sirna tulasi (Telugu), bilati tulas .The phytoconstituent present in Hyptis is Alkaloid, Flavonoid, Terpenoid, Phenollic compound.It also shows the pharmacological effect like Anti-cancerous, Anti-microbial,Anti-oxidative, Anti-plasmodic,etc activity.The plant is aromatic.

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<em>In vivo</em> antimalarial activity of the ethanolic leaf extract of <em>Hyptis suaveolens</em> poit on <em>Plasmodium berghei</em> in Mice

Cited by 5 publications

References 21 publications

Isolation and structure elucidation of anti-malarial principles from Terminalia mantaly H. Perrier stem bark

Isolation and structure elucidation of anti-malarial principles from Terminalia mantaly H. Perrier stem bark

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The Phytochemical and Pharmacological Screening of Hyptis Suaveolens

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