The aim of this study was to prepare and evaluate gels incorporating nanostructured lipid carriers (NLC) of Miconazole nitrate (MN) for systemic delivery of the active after topical application. MN has been used as model drugs to be incorporated into nanostructured lipid carriers, once they are very well established as antimycotics for the treatment of topical fungal infections. NLC designed for topical administration of MN, were prepared by the hot high pressure homogenization technique. This MN-NLC was characterized for particle size, entrapment efficiency, and SEM. The lipid nanoparticles were incorporated in gels for convenient topical application and were evaluated forfor particle size, Rheological analysis Texture analysis , In vitro drug release studies and Ex Vitro skin permeation Studies. The preparation of aqueous NLC dispersions with a mean particle size lower than 300 nm has been obtained with uniform size distribution (PI < 0.350). The prepared semi-solid systems showed mean particle size remained lower than 250 nm and PI remained lower than 0.500 after 3 months of storage. An initial rapid release was observed in the case of Marketed gel, whereas MN-NLC Gel depicted a slow initial release with a lag time of 0.5 h and 1 h, respectively. High amount of MN release was facilitated through abdominal skin of rats from marketed gel than MN-NLC Gel. Research work could be concluded as successful development of MN-loaded NLC-bearing hydrogel to increase the encapsulation efficiency of colloidal lipid carriers with advantage of improved performance in terms of stability and provides a sustaining MN topical effect as well as faster relief from fungal infection.

The main aim of this article is to know the importance of traditional plant i.e Hyptis Suaveolens. It belong to family Lamiaceace, commonly called as Wialaitis tulsi and it grow on sub-tropic and semi- arid environment. It use broadly and belong to Subkingdom – Tracheobionta, Super kingdom – Spermatophyte , Division – Magnoliophyte, Class – Magnoliopsida, Subclass – Asteridae, Order – Lamiales. Hyptis is widespread in Australia its perennial, annual ,herb or sub-herb. The leaves of Hyptis is opposite and ovate about 2.5 to 10cm in length. The flower is dark purple in color and auxiliary with long stalk, hairy calyx and about 4mm long. The seeds are flat and mucilaginous fruit (nut let) are about 1.2-1.5mm long cure swellings, abscesses and hemorrhoids. In India the plant is considered to be stimulant, \carminative, soporifics and lactogogue.Commonly it is called as Horehound, Pignut, Wild spikenard, Gross Baume, Hyptis odder (French), Alfavaca-brava, Betônica-brava (Portuguese, Brazil), Chao, Hierba de las mules, hortela do campo (Spanish), Wilayati tulsi (Hindi), bhustrena, darp tulas, jungli tulas (Marathi), sirna tulasi (Telugu), bilati tulas .The phytoconstituent present in Hyptis is Alkaloid, Flavonoid, Terpenoid, Phenollic compound.It also shows the pharmacological effect like Anti-cancerous, Anti-microbial,Anti-oxidative, Anti-plasmodic,etc activity.The plant is aromatic.

The intracellular accumulation of anti-cancer agents strongly influences the efficiency of chemotherapy for cancer. In the present research work, a simple rapid, sensitive reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed and validated to determine free, encapsulated and total drug in Doxorubicin liposome a cytotoxic Nano pharmaceutical formulation. The free drug and total drug were measured by RP-HPLC with a C18 column after extraction with waters Oasis SPE cartridge using solid phase extraction assembly with methanol and mobile phase as diluent. The mobile phase contained 0.1% trifluro acetic acid and solvent mixture of acetonitrile and methanol in the ratio of 80:20. UV/PDA detector with wavelength 254 nm was used for determination of free and encapsulated drug. The calibration curve was linear from 5 to 100 µg/ml with correlation coefficient of 0.999 while the recovery of total assay was between 98% and 101%. The intra and inter day coefficient of variation (RSD) were less than 1%. Furthermore, the validated method was used to determine the free, encapsulated and total drug for the developmental liposome as Nano pharmaceutical formulation.

This study presents the development of gradient reverse phase high performance liquid chromatographic method for the determination of Doxorubicin hydrochloride and its five impurities following Quality by Design (QbD) approach and also to identify the conditions where adequate separation quality in minimal analysis duration could be achieved within a robust region that guarantees the stability of method performance. The relationship between critical process parameters and critical quality attributes is created applying Design of Experiments methodology. The defined mathematical models and central composite design are used to evaluate the risk of uncertainty in models prediction and concerns in adjusting the process parameters and to identify the design space. Moreover, Box-Behnkan design is applied for experimental robustness testing and method is partially validated to verify the adequacy of selected optimal conditions. The analytical column Waters X-bridge C18 (250 mm x 4.6 mm, 5µm particle size); mobile phase A consisted of buffer (100 mM Sodium dodecyl sulphate and 22mM of Orthophosphoric acid , pH adjusted to 2.5 with 1 M sodium hydroxide solution), acetonitrile and methanol (60:30:10) v/v/v; mobile phase B consisted of Buffer, acetonitrile and methanol (30:60:10),column temperature 40ºC, mobile phase flow rate 1 mL min-1 , wavelength of detection 254 nm..