<i>Bacillus subtilis</i> 168 as a unique platform enabling synthesis and dissemination of genomes

Integrated conjugal plasmid pLS20 in the Bacillus subtilis genome produced 850‐kbp circular subgenomes transmissible to another B. subtilis

“…In addition, the conjugation gene system of pLS20 (Singh et al, 2013) should allow the transmission of up to 850‐kbp subgenomes at relatively high rates. Large amounts of genome DNA synthesized previously are primarily found in the proB locus (Figure 2a) (Itaya, 2022). The present results finally made our BGM system streamlined from genome synthesis to transmission (Figure 1).…”

“…Genome synthesis has been developed for the production of novel genomes (Itaya, 2022). We have provided Bacillus subtilis as a genome vector, called BGM, to enable the synthesis and manipulation of a wide range of giant genome segments (Itaya et al, 2005(Itaya et al, , 2008.…”

“…Streamlined technology from genome synthesis to genome transmission developed by the use of B. subtilis . Upper: A basic pipeline in our previous review is redrawn to clarify two steps “Genome Dissection” and “Dissemination” (Itaya, 2022). Transmission by conjugational transfer is emphasized by a red curved arrow.…”

Biocontrol of bacterial wilt disease in tomato using Bacillus subtilis strain R31

“…Adding R31 immediately without preculturing R. solanacearum inhibited the growth of R. solanacearum . However, strain 168, a type strain of B. subtilis ( Itaya, 2022 ), did not directly inhibit the growth of R. solanacearum ( Figure 3A ). Notably, when R31 was added after 12 h of R. solanacearum culture, the inhibitory effect of this strain on R. solanacearum growth was significantly diminished.…”

“…Bacillus subtilis strains R31 and 168 ( Itaya, 2022 ) were preserved in our laboratory. The R31 strain was activated on an LB agar plate and cultured at 37°C for 12 h. A single colony was selected, inoculated into 5 mL of LB liquid medium, and cultured at 37°C for 12–14 h. A 50-μL aliquot of inoculum was transferred into 5 mL of LB medium and cultured at 37°C for 4 h. Subsequently, 12 mL of inoculum was transferred into 200 mL of nutrient broth (NB) medium ( Sangare et al, 2014 ) and cultured at 37°C for 48 h to obtain the fermentation broth.…”