&lt;i&gt;In-vitro&lt;/i&gt; and &lt;i&gt;In-vivo&lt;/i&gt; Antiasthmatic Studies of &lt;i&gt;Ailanthus excelsa&lt;/i&gt; Roxb. on Guinea Pigs

Kumar, D.; Bhujbal, Santosh S.; Deoda, Ramesh S.; Mudgade, S. C

doi:10.3329/jsr.v2i1.2740

Cited by 24 publications

(18 citation statements)

References 10 publications

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“…Phytobiotics is a term used to describe plant-derived natural bioactive compounds that promote livestock health and well-being, but their bioavailability is not yet clear (Wu and Wu, 2012). Many studies have employed the in vitro and in vivo screening models to evaluate the effects of plant extracts on pigs (Calvo et al, 2006;Kumar et al, 2010;Verhelst et al, 2014). In vitro studies directly evaluate product performance, while in vivo tests can suffer from complications due to the indirect approach (Polli, 2008).…”

Section: Discussionmentioning

confidence: 99%

Modulation of Plasma Antioxidant Activity in Weaned Piglets by Plant Polyphenols

Zhang

Jiang

Mantovani

et al. 2014

Italian Journal of Animal Science

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This study was conducted to evaluate the effect of plant polyphenols (PP) on antioxidant activity in weaned piglets. First, a uniform design, one optimising an experimental technique that can rationally arrange the concentrations of mixture components, was used to obtain the best PP mixture of apple, grape seed, green tea and olive leaf polyphenols based on in vitro antioxidant capacity and inhibitory action on bacterial growth. Second, the optimised PP mixture was tested in vivo with an efficacy trial on piglets. The optimal effects of the mix were observed in vitro when apple, grape seed, green tea, olive leaf polyphenols and a carrier (silicon dioxide) accounted for 16.5, 27.5, 30, 2.5 and 23.5%, respectively, of the mixture. Forty-eight weaned piglets were randomly allocated to two dietary treatments (6 replicates of 4 piglets each per treatment) and fed a control diet (CTR) or CTR supplemented with 0.1% of the optimised PP mixture. Dietary PP did not affect growth performance compared to the CTR group. Plasma total protein, urea nitrogen and lysozyme content were not affected by dietary treatment. No differences of E. coli or Clostridia counts in the faeces and caecum content between the CTR and PP groups were observed. A reduced malondialdehyde concentration in the PP group was observed on day 21 compared to the CTR group (P=0.02). In conclusion, the prepared PP mixture has the potential to improve plasma antioxidant activity.

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Section: Discussionmentioning

confidence: 99%

Modulation of Plasma Antioxidant Activity in Weaned Piglets by Plant Polyphenols

Zhang

Jiang

Mantovani

et al. 2014

Italian Journal of Animal Science

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“…DOI: 10.3109/13880209.2013.869232 that causes asphyxia and death. Bronchodilators can delay the occurrence of these symptoms (Kumar et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Pharmacological evaluation for anti-asthmatic and anti-inflammatory potential ofWoodfordia fruticosaflower extracts

Ghante¹,

Bhusari²,

Duragkar³

et al. 2014

Pharmaceutical Biology

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Context: Woodfordia fruticosa Kurz. (Lythraceae) flowers are ethnopharmacologically acclaimed in the Indian medicinal system to treat asthma. Objective: To evaluate W. fruticosa flower extracts for anti-asthmatic effect. Materials and methods: Ethyl acetate, acetone, methanol, and hydro-alcohol extracts of W. fruticosa flowers were obtained successively and standardized. Ability of extracts to stabilize free radicals and compound-48/80-induced mast cell degranulation was evaluated. In vitro anti-inflammatory potential of extracts at 100 and 200 mg/ml by membrane stabilization and in vivo inhibition of rat paw edema up to 5 h (100 and 200 mg/ml; p.o.) was evaluated. In vitro bronchorelaxant effect was examined against histamine-and acetylcholine (1 mg/ml; independently)-induced guinea pig tracheal contraction. Extracts were evaluated for bronchoprotection (in vivo) ability against 0.1% histamine-and 2% acetylcholine-induced bronchospasm in guinea pigs at 100 and 200 mg/ml; p.o. Results: Standardization studies revealed that the methanol extract exhibited highest polyphenolic (62.66 GAE), and flavonoid (6.32 RE) content and HPLC fingerprinting confirmed the presence of gallic acid (R t 1.383). IC 50 values for DPPH scavenging and metal chelation by the methanol extract were 40.42 and 31.50 mg/ml. Methanol and ethyl acetate extracts at 100 mg/ml exhibited 06.52 and 07.12% of histamine release. Methanol, ethyl acetate, and hydro alcohol extracts at 200 mg/kg demonstrated 32. 73, 29.83, 26.75% and 32.46, 9.38, 26.75% inhibition of egg albumin and carrageenan-induced inflammation, respectively. Methanol extract exhibited 100% bronchorelaxation and 48.83% bronchoprotection. Conclusion: Woodfordia fruticosa flower (WFF) extracts exhibited anti-asthmatic effect by demonstrating bronchoprotection, bronchorelaxation, anti-inflammatory, antioxidant, and mast cell stabilization ability.

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“…Bronchodilators can delay the occurrence of these symptoms. 24 In animals, coughing has been elicited by number of methods. [25][26][27][28] In the present study, the anti-tussive activity of EEWF has been compared with that of standard drug codeine against coughing induced by chemical stimulation (citric acid).…”

Section: Discussionmentioning

confidence: 99%

Phytopharmacological evaluation of aerial parts of Woodfordia fruticosa (L.) Kurz in Cough Variant Asthma

Srivastava¹,

Siddharth²,

Hemant³

et al. 2015

Phcog J

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Background: Cough variant asthma (CVA) is characterized by prolonged non productive cough which responds to bronchodilator therapy. None of herbal drug is reported to possess pharmacological activity against CVA. Objective: To investigate the pharmacological potential of ethanolic extract of Woodfordia fruticosa (L.) Kurz (EEWF) against CVA as well as to develop an efficient screening model for CVA. Material and Method: Anti-tussive effect of EEWF was evaluated against nebulized aqueous solution of 0.1 g/ml of citric acid to determine the cough response. EEWF potential was finally accessed against aerosolic mixture of 0.3 g/ml of citric acid mixed with 0.1% histamine and 2% acetylcholine chloride to evaluate the convulsive latency, percentage protection and cough frequency against CVA. Results: EEWF at aerosolic dose of 6% w/v exhibit decrease in of the average coughs frequency (4.83 ± 0.30) which is quite significant effect as compared to standard drug codeine. EEWF against aerosol induced CVA was found to exhibit a significant bronchoprotection of 41.75% and decreases number of coughs SUMMARY• The present study investigated a potential curative effect of ethanolic extract of Woodfordia fruticosa (L.) Kurz in Aerosol induced Cough Variant Asthma.• As none of any model exists to assess the activity of the drug for CVA, so our study also aimed to develop an effective pharmacological screening model for CVA.• The extract not only protects the animals from bron¬choconstriction and bronchospasm but also suppress the cough frequency, which almost covers the basic etiological relevance for CVA, which pharmacologically corroborates its effective treatment against CVA.

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<i>In-vitro</i> and <i>In-vivo</i> Antiasthmatic Studies of <i>Ailanthus excelsa</i> Roxb. on Guinea Pigs

Abstract: Methanolic extract of stem barks of Ailanthus excelsa Roxb. was evaluated for antiasthmatic activity by employing in-vivo and in-vitro screening models in Guinea pigs. The results revealed that the methanolic extract produced significant dose-dependent antiasthmatic activity.

Cited by 24 publications

References 10 publications

Modulation of Plasma Antioxidant Activity in Weaned Piglets by Plant Polyphenols

Modulation of Plasma Antioxidant Activity in Weaned Piglets by Plant Polyphenols

Pharmacological evaluation for anti-asthmatic and anti-inflammatory potential ofWoodfordia fruticosaflower extracts

Phytopharmacological evaluation of aerial parts of Woodfordia fruticosa (L.) Kurz in Cough Variant Asthma

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