&lt;i&gt;In vitro&lt;/i&gt; Diagnosis of Atopic Allergy

Johansson, S. G. O.; Bennich, H.; Berg, T.

doi:10.1159/000230538

Cited by 122 publications

(4 citation statements)

References 6 publications

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“…The amount of a fixed quantity of IgE antibody in the standard could be compared to the unknown quantity of specific IgE antibody in the test sample by comparison of the c.p.m. This is a similar approach to that described by Johansson, Bennich & Berg, 1971. Calculations wer peerformed as follows:…”

Section: (B) Skin Testsmentioning

confidence: 99%

Comparison of the allergenic properties of bee venom and whole bee body extract

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Reisman

Arbesman

1976

Clin Experimental Allergy

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The allergenic properties of bee venom and whole bee body extract were compared by in vivo and in vitro tests. The majority of patients with knowrLbee sting sensitivity had positive intracutaneous skin test reactions with bee venom and had bee venom specific IgE in their sera. Of seventeen patients with positive bee venom skin tests, nine had positive tests with whole bee body extract. Of thirty sera containing elevated levels of bee venom specific IgE obtained from untreated patients, fourteen sera contained whole body specific IgE but in much lower titres.In RAST inhibition experiments using both bee venom and whole bee body extract as couphng antigens, bee venom was a more potent inhibiting antigen than whole body extract.From these experiments we conclude that bee venom is a more potent allergen than whole bee body extract.

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Section: (B) Skin Testsmentioning

confidence: 99%

Comparison of the allergenic properties of bee venom and whole bee body extract

Light

Reisman

Arbesman

1976

Clin Experimental Allergy

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“…M easurement of reagins. Serum IgE was measured by the radioimmunosorbent technique (I I) and the reagins against cow•s milk, cereals and egg white were measured by the radioallergosorbent test (RASn according to Johansson et al (4). All tests were done at the Blood Centre, University Hospital.…”

Section: Methodsmentioning

confidence: 99%

Provocation with cow's milk and cereals in atopic dermatitis

Hammar¹

1977

Acta Derm Venereol

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Oral provocation test with cow's milk and cereals were performed in 81 children with atopic dermatitis, all below 5 years of age. In 15 of the children exacerbation of the dermatitis occurred on the second or third day after initiation of the tests. These patients, whose mean age was 13 months, underwent repeated provocation during the ensuring 18 months. Eleven children lost their sensitivity to the offending foods and 2 children became more tolerant during this period. Initial levels of serum IgE and specific reagins against the foods tested did not distinguish those patients who were positive to the provocation tests. In this group, however, reagins against egg white were significantly increased. The specific serum reagins were unaltered or increased in the majority of cases during the period of study. The radioallergosorbent test (RAST) was found of limited value as a screening tool in patients with atopic dermatitis in whom exacerbation of the condition appears to be caused by food sensitivity.

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“…Bound IgE was detected with 125I-labelled rabbit anti-human IgE, raised against the YU IgE myeloma, purified and iodinated as described by Johansson et al (1971). Assay methodology used and calculations of the results have been described in detail by Gleich et al (1974) and Chakrabarty et al (1981).…”

Section: Electrophoretic Analysismentioning

confidence: 99%

Physicochemical and immunochemical characterization of allergenic proteins from rye-grass (Lolium perenne) pollen prepared by a rapid and efficient purification method

Cottam¹,

Moran²,

Standring³

1986

Biochemical Journal

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Three fractions of rye-grass (Lolium perenne) pollen extract have been isolated by preparative isoelectric focusing (i.e.f.) and characterized in terms of physicochemical and immunochemical properties. The purified components were designated 'R7' and 'R14' on the basis of their positions in relation to other rye-grass pollen extract components on SDS/polyacrylamide-gel electrophoresis and their apparent molecular masses were assessed as 31 and 11 kDa respectively. On i.e.f., R14 split into two components, one acidic (pl 5.0) and one basic (pl 9.0), termed 'Rl4a' and 'Rl4b' respectively, and R7 focused at pl 5.8. R7 and R14a were shown to be allergenic by skin-prick test and all three components were recognized by rye-grass-pollen-specific human IgE. On SDS/polyacrylamide-gel electrophoresis and i.e.f., R7 behaved in a manner identical with that shown by an authentic sample of Rye I and gave an amino acid analysis similar to published data Immunochemistry 3, 91-100] for Rye group-I isoallergens; the amino acid sequence of the first 27 N-terminal amino acids was also determined. Physicochemical analysis revealed that R14a was equivalent to Rye II and 14b to Rye III. Preparative i.e.f. followed by gel-permeation chromatography proved to be a rapid and efficient method for purifying the allergenic components of Rye I (R7), Rye II (Rl4a) and Rye III (Rl4b) from rye-grass pollen extract.

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<i>In vitro</i> Diagnosis of Atopic Allergy

Cited by 122 publications

References 6 publications

Comparison of the allergenic properties of bee venom and whole bee body extract

Comparison of the allergenic properties of bee venom and whole bee body extract

Provocation with cow's milk and cereals in atopic dermatitis

Physicochemical and immunochemical characterization of allergenic proteins from rye-grass (Lolium perenne) pollen prepared by a rapid and efficient purification method

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