&lt;I&gt;In Vitro&lt;/I&gt; Phototoxicity of Liposomes and Nanocapsules Containing Chloroaluminum Phthalocyanine on Human Melanoma Cell Line

Barbugli, Paula Aboud; Siqueira-Moura, Marigilson P.; Espreáfico, Enilza Maria; Tedesco, Antônio Cláudio

doi:10.1166/jnn.2010.1741

Cited by 39 publications

(31 citation statements)

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“…The results of our particle size analysis indicated that the mean size of the particles used in our system is in this adequate nanometric range (average diameter of 8 nm). The surface charge or zeta potential (ζ) is the electrokinetic potential formed by an ionic double layer that is susceptive to a voltage difference in millivolts [40]. The measurement of ζ allows predictions of the stability of colloidal aqueous dispersions.…”

Section: Discussionmentioning

confidence: 99%

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Taboga¹

2014

J Nanomed Nanotechnol

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Nanotherapy applied to cancer treatment is constantly evolving, and new approaches to current techniques, such as magnetohyperthermia, are being implemented to solve and minimize the limitations of conventional therapeutic strategies. The purpose of this study was to investigate the action of polyphosphate-coated maghemite nanoparticles (MNPs) on oral squamous cell carcinoma. Human oral cancer cells (UM-SCC14A) were incubated with MNPs at various concentrations and subjected to cell proliferation tests (MTT), apoptosis assays and transmission electron image analysis. Viability and apoptotic events were time and dose dependent. These in vitro tests showed that at the intermediate concentration tested there is no significant toxicity, as confirmed by transmission electron microscopy. For this reason this MNPs concentration was chosen for the subsequent in vivo tests. Oral tumor induction was performed by applying the carcinogen DMBA to Syrian hamsters. Animals were then treated by magnetohyperthermia using MNPs. No signs of general clinical symptoms of toxicity or abnormal behavioral reactions were observed. However, animals treated with MNPs and exposed to the alternating magnetic field in the hyperthermia procedure exhibited a significant and time dependent cancer regression, as confirmed by histopathological analyses and immunohistochemistry. Actually, in quantitative terms of the magnetotherapy efficacy involving these polyphosphate-coated MNPs, 100% recovery (12/12) was observed in the oral cancer tumor bearing Syrian hamsters seven days after the treatment with the magnetohyperthermia procedure. Data supports the suggestion that the MNPs-mediated hyperthermia represents a promising strategy for the treatment of oral cancer.in patients with malignancies in the mouth region. Unlike many other oncology subspecialties, however, the rates of recurrence and overall survival have improved only modestly over the past decades, despite the continuous search for new therapeutic techniques [6,7]. Thus, it is expected that the identification and use of specific agents will lead to individualized treatments, which in turn will lead to significantly better results as manifested by more cures and better quality of life by decreasing toxicity [8].Over the last years, maghemite nanoparticles (MNPs) have appeared as a well-established technology and an important field of research in superparamagnetism of colloidal materials, characteristic which allows them to be guided with an external magnetic field [9]. Recently, MNPs have been investigated for therapeutic purposes such as hyperthermic treatment [10,11]. Magnetohyperthermia (MHT) represents a novel and promising therapy for cancer treatment [11,12], being capable of promoting the specific lysis of tumor cells, thereby improving patient outcomes while minimizing the subsequent toxicity effects [13]. The Citation: Candido NM, Calmon MF, Taboga SR, Bonilha JL, dos Santos MC, et al. (2014) High Efficacy in Hyperthermia-associated with Polyphosphate

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Section: Discussionmentioning

confidence: 99%

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Taboga¹

2014

J Nanomed Nanotechnol

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“…The cellular culture was accomplished as described by Barbugli et al 14 The cells were treated with five formulations (1 ¼ drug-free nanocapsule þ ionic magnetic fluid at 5 Â 10 9 particle/ml; 2 ¼ Selol-loaded nanocapsules at 100 lg/ml; 3 ¼ Selol-loaded nanocapsule at 25 lg/ ml þ ionic magnetic fluid at 1.25 Â 10 9 particle/ml; 4 ¼ Selolloaded nanocapsule at 50 lg/ml þ ionic magnetic fluid at 2.5 Â 10 9 particle/ml; and 5 ¼ Selol-loaded nanocapsule at 100 lg/ml þ ionic magnetic fluid at 5 Â 10 9 particle/ml) for 3 h in an incubator (5% CO 2 , 37 C, humidified atmosphere). Subsequently, the medium with formulations was removed.…”

Section: Methodsmentioning

confidence: 99%

Selol-loaded magnetic nanocapsules: A new approach for hyperthermia cancer therapy

Falqueiro

Primo

Morais

et al. 2011

Journal of Applied Physics

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Polylactic-co-glycolic nanocapsules, loaded with nanosized magnetic particles and Selol (a selenium-based anticancer drug), were successfully prepared by the precipitation method. Maghemite (γ-Fe2O3) nanoparticles were incorporated into the nanocapsules using a highly stable ionic magnetic fluid sample. The obtained nanocapsules presented no agglomeration, negative surface charge while revealing a narrow monomodal size distribution. All the nanocapsule formulations exhibited a good physical stability at 4 °C during 3 month storage period. The in vitro antitumoral activity of Selol-magnetic nanocapsules was assessed using a murine melanoma cell line. The influence of nanocapsules on cell viability was investigated by spectrophotometric assay. The results demonstrated that Selol-loaded magnetic nanocapsules (at 100 μg/ml/5 × 109 particle/ml) showed antitumoral activity of 50% on melanoma cells (absence of magnetic field). These results clearly indicate that the loaded nanocapsules represent a novel and promising magnetic drug delivery system suitable for cancer treatment via the active drug and magnetohyperthermia.

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“…Among them, phthalocyanines (Pc) have been under investigation as promising second-generation photosensitizers, not only for PDT, but also for other applications. Because of their strong red-shifted absorption band, associated with their photophysical response, they are well suited to take advantage of maximal tissue penetration and low-light scattering properties of organelles and tissue at the ideal phototherapeutic window (600-800 nm).For instance, aluminum chloride phthalocyanine presents an intense absorption peak at 670 nm, and, therefore, diode laser operating at this wavelength is required [30,31].…”

Section: Interaction Of Light In the Skinmentioning

confidence: 99%

Low Level Energy Photodynamic Therapy for Skin Processes and Regeneration

Tedesco¹,

Jesus²

2017

Photomedicine - Advances in Clinical Practice

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Skin is the largest human organ and displays multiple functions involving structure and protection against external agents that may affect the body. Solar radiation accelerates the normal aging process and may even cause great damage leading to many different cutaneous diseases and skin cancer. Moreover, a wound in the skin may be an open channel for the access of pathogens usually exposing blood vessels for infections and causing serious complications. For many reasons, regulatory skin processes are of great deal in different approaches: basic antiaging, wound healing, and skin cancer. In a good way, photoprocesses with specific wavelength at low energy levels associated with photoactive compounds are known to cause the opposite effect, promoting the healing of cutaneous diseases and leading to well-defined outcomes in rejuvenation and antiaging. This chapter will discuss the most relevant topics in photo skin regeneration using low energy levels associated with photodynamic therapy (PDT), which emerged as a combination to potentialize molecules with the already known effects of PDT and low level laser therapy (LLLT) in the treatment of skin pathologies, known as a photobiomodulation process.

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<I>In Vitro</I> Phototoxicity of Liposomes and Nanocapsules Containing Chloroaluminum Phthalocyanine on Human Melanoma Cell Line

Cited by 39 publications

References 0 publications

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

High Efficacy in Hyperthermia-associated with Polyphosphate Magnetic Nanoparticles for Oral Cancer Treatment

Selol-loaded magnetic nanocapsules: A new approach for hyperthermia cancer therapy

Low Level Energy Photodynamic Therapy for Skin Processes and Regeneration

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