&lt;i&gt;In Vitro&lt;/i&gt; Regeneration of Coconut Plantlets from Immature Inflorescence

Shareefa, M.; Thomas, Rachel; Sreelekshmi, J. S.; Rajesh, M. K.; Karun, Anitha

doi:10.18520/cs/v117/i5/813-820

Cited by 13 publications

(11 citation statements)

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“…The use of inflorescence tissues from C. sativa appears to be a promising alternative mode of micropropagation to nodal cultures [74,76], having been well studied in many species [62,[96][97][98]. Inflorescences tissues that demonstrate the ability to return from a flowering phase of growth to a vegetative stage of growth are broadly described as undergoing floral or inflorescence reversion [62,[96][97][98][99]. In Vitro PGR-induced floral reversion has been shown in a variety of dicots and monocots.…”

Section: Floral Reversion: An Alternate Micropropagation Approachmentioning

confidence: 99%

“…In Vitro PGR-induced floral reversion has been shown in a variety of dicots and monocots. In monocots, it is widely used in many commercially important crops such as grasses, palms, bananas, and grains [62,98,[100][101][102]. In dicots, floral reversion has been employed less frequently; however, it has been shown in the Brassicaceae family and has been employed in conservation efforts of recalcitrant dicots [96,97,103,104].…”

Section: Floral Reversion: An Alternate Micropropagation Approachmentioning

confidence: 99%

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The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

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Section: Floral Reversion: An Alternate Micropropagation Approachmentioning

confidence: 99%

Section: Floral Reversion: An Alternate Micropropagation Approachmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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show abstract

“…The use of inflorescence tissues from C. sativa appears to be a promising alternative mode of micropropagation to nodal cultures [74,76] having been well studied in many species. Inflorescences tissues which demonstrate the ability to return from a flowering phase of growth to a vegetative stage of growth are broadly described as undergoing floral or inflorescence reversion [62,[96][97][98][99]. In vitro PGR-induced floral reversion has been shown in a variety of dicots and monocots.…”

Section: Floral Reversion: An Alternate Micropropagation Approachmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony¹,

Page²,

Hesami³

et al. 2020

Preprint

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect/disease/virus free clonal plants, store germplasm, and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history or prohibitions and restrictions. Existing literature has multiple limitation: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity, studies using drug-type cultivars are predominantly op-timized using a single cultivar, most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems the multiplication phase of micropropagation (stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

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“…One alternative to nodal culture is the use of Cannabis inflorescences as starting material for multiplication. Floral reversion is a process in which floral tissues revert to a vegetative state and has been demonstrated in vitro across species from many taxa (Eapen and George 1997; Phulwaria and Shekhawat 2013; Punyarani et al 2013; Shareefa et al 2019). In the development of cell suspension cultures, Raharjo et al .…”

Section: Introductionmentioning

confidence: 99%

Flower Power: Floral reversion as a viable alternative to nodal micropropagation inCannabis sativa

Monthony

Bagheri

Zheng

et al. 2020

Preprint

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The legalization of Cannabis sativa L. for recreational and medical purposes has been gaining global momentum, leading to a rise in interest in Cannabis tissue culture as growers look for large-scale solutions to germplasm storage and clean plant propagation. Mother plants used in commercial propagation are susceptible to insect pests and disease and require considerable space. While micropropagation can produce disease free starting material in less space, current published in vitro micropropagation methods are not robust and few report high multiplication rates. Further, these micropropagation methods rely on photoperiod-sensitive plants which can be maintained in a perpetual vegetative state. Current methods are not adaptable to long-term tissue culture of day-neutral cultivars, which cannot be maintained in perpetual vegetative growth. In this study, we chose to develop a micropropagation system which uses C. sativa inflorescences as starting materials. This study used two cannabis cultivars, two plant growth regulators (PGR; 6-benzylaminopurine and meta-topolin) at different concentrations, and two different numbers of florets. Here we show that floral reversion occurs from meristematic tissue in C. sativa florets and that it can be used to enhance multiplication rates compared to existing in vitro methods. Floret number was shown to have a significant impact on percent reversion, with pairs of florets reverting more frequently and producing healthier explants than single florets, while cultivar and PGR had no significant effect on percent reversion. Compared with our previously published nodal culture studies, the current floral reversion method produced up to eight times more explants per tissue culture cycle. Floral reversion provides a foundation for effective inflorescence-based micropropagation systems in C. sativa.

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<i>In Vitro</i> Regeneration of Coconut Plantlets from Immature Inflorescence

Cited by 13 publications

References 0 publications

The Past, Present and Future of Cannabis sativa Tissue Culture

The Past, Present and Future of Cannabis sativa Tissue Culture

The Past, Present and Future of Cannabis sativa Tissue Culture

Flower Power: Floral reversion as a viable alternative to nodal micropropagation inCannabis sativa

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