&lt;i&gt;In vitro&lt;/i&gt; Synergy and Time-kill Assessment of Interaction between Kanamycin and Metronidazole against Resistant Bacteria

Ammari, Wesam G.

doi:10.4314/tjpr.v14i5.14

Cited by 4 publications

(1 citation statement)

References 18 publications

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“…Quercetin combined with some polyphenolic acids (e.g., gallic acid, cinnamic acid) has a synergistic antibacterial effect against Aeromonas aeruginosa and has an additive effect on Edwardsiella tarda and Aeromonas hydrophila (Prasad et al, 2014). For Escherichia coli , the synergistic antibacterial effect of kanamycin combines well with metronidazole (Olajuyigbe and Afolayan, 2015), ciprofloxacin, and the alkaloid extract of Sophora alopecuroides (Jaktaji and Mohammadi, 2018). The combination of Berberis aristata , colistin, and tigecycline has a synergistic effect on carbapenem-resistant E. coli (Thakur et al, 2016).…”

Section: Application Of Isobologram Analysis To Evaluate Drug Interacmentioning

confidence: 99%

Isobologram Analysis: A Comprehensive Review of Methodology and Current Research

et al. 2019

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Drug combination is a common method for clinical disease treatment. Whether the combination of drugs is reasonable often affects the result of the disease treatment. Many methods have been used to evaluate interaction between drugs to date. Isobologram analysis has been mathematically proven and widely used to evaluate drug interactions. In this paper, the principle of isobologram analysis and its application in drug interaction evaluation are summarized. The applications of the similar cotoxicity coefficient and fractional inhibitory concentration index in the evaluation of drug interaction are also reviewed. This work is expected to evaluate the effect of formulations scientifically and provide scientific judgment standards for the development of formulations and clinical drug compatibility.

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Section: Application Of Isobologram Analysis To Evaluate Drug Interacmentioning

confidence: 99%

Isobologram Analysis: A Comprehensive Review of Methodology and Current Research

et al. 2019

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Synergistic Effect of Thymol–Ciprofloxacin Combination on Planktonic Cells and Biofilm of Pseudomonas aeruginosa

Jayakumar,

Mini,

Kumar

et al. 2023

Curr Microbiol

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New combination approaches to combat methicillin-resistant Staphylococcus aureus (MRSA)

Sharaf

El-Sherbiny

Moghannem

et al. 2021

Sci Rep

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The herbal products proved to be more promising antimicrobials even though their antimicrobial activity is milder than commercially available antibiotics. Moreover, herbal drugs may act synergistically with antibiotics to kill microbes. In this study, we aimed to enhance the activity of penicillin against MRSA through combination with the active saponin fraction isolated from the Zygophyllum album plant. Three different types of metabolites (saponins, sterols, and phenolics) have been extracted from Zygophyllum album with ethanol and purified using different chromatographic techniques. The antibacterial activity of crude extract and the separated metabolites were checked against MRSA isolates, Saponin fraction (ZA-S) was only the active one followed by the crude extract. Therefore, the compounds in this fraction were identified using ultra-high-performance liquid chromatography connected to quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) operated in positive and negative ionization modes. UHPLC/QTOF-MS revealed the presence of major six ursane-type tritepenoidal saponins (Quinovic acid, Quinovic acid 3β-O-β-D-quinovopyranoside, Zygophylloside C, Zygophylloside G, Zygophylloside K and Ursolic acid), in addition to Oleanolic acid. Interaction studies between saponin fraction and penicillin against MRSA were performed through the checkerboard method and time-kill assay. According to checkerboard results, only three combinations showed a fractional inhibitory concentration index less than 0.5 at concentrations of (62.5 + 312.5, 62.5 + 156.25, and 62.5 + 78.125 of penicillin and ZA-S, respectively). Time kill assay results showed that the highest reduction in log10 colony-forming unit (CFU)/ml of initial inoculum of MRSA after 24 h occurred by 3.7 at concentrations of 62.5 + 312.5 (µg/µg)/ml of penicillin and ZA-S, respectively. Thus, the combination between saponin fraction of Zygophyllum album and penicillin with these concentrations could be a potential agent against MRSA that can serve as possible model for new antibacterial drug.

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<i>In vitro</i> Synergy and Time-kill Assessment of Interaction between Kanamycin and Metronidazole against Resistant Bacteria

Cited by 4 publications

References 18 publications

Isobologram Analysis: A Comprehensive Review of Methodology and Current Research

Isobologram Analysis: A Comprehensive Review of Methodology and Current Research

Synergistic Effect of Thymol–Ciprofloxacin Combination on Planktonic Cells and Biofilm of Pseudomonas aeruginosa

New combination approaches to combat methicillin-resistant Staphylococcus aureus (MRSA)

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