<i>KLOTHO</i> Gene Polymorphisms G-395A and C1818T Are Associated with Low-Density Lipoprotein Cholesterol and Uric Acid in Japanese Hemodialysis Patients

Abstract

Background/Aims: This study aimed to investigate the association of the KLOTHO gene single nucleotide polymorphisms (SNPs), G-395A and C1818T, with various laboratory data in 219 Japanese hemodialysis (HD) patients. Methods: The genotyping of G-395A in the promoter region and C1818T in exon 4 was performed using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay. Results: In HD patients, the allele frequencies of G-395A were 0.847 for the G allele and 0.153 for the A allele and those of the C1818T were 0.829 for the C allele and 0.171 for the T allele. There were no significant differences in allele frequencies of G-395A and those of the C1818T between HD patients and healthy subjects. Multivariate analysis adjusted for age and duration on HD demonstrated that uric acid was significantly high in A allele carriers of G-395A compared with GG genotype in all and female patients. Low-density lipoprotein cholesterol was significantly low in T allele carriers of C1818T compared with CC genotype in all and male patients. Conclusion:KLOTHO gene SNPs G-395A and C1818T are associated with low-density lipoprotein cholesterol and uric acid in HD patients.

Biochemical and Functional Characterization of the Klotho-VS Polymorphism Implicated in Aging and Disease Risk

“…GWAS have revealed more common alterations in KL that do not cause overt and disabling disease but do affect disease risk. Two of these variants, G395A in the promoter region and C1818T in exon 4 of KL, are associated with increased risk of cardiovascular disease, priapism, nephropathy, osteoarthritis, diabetes, and increased bone mineral density (41)(42)(43)(44)(45)(46)(47)(48)(49). Multiple studies have also shown an association of KLVS with both longevity and disease risk.…”

FGF-23, α-Klotho Gene Polymorphism and Their Relationship with the Markers of Bone Metabolism in Chronic Peritoneal Dialysis Patients

“…Klotho gene is also known to have some anti-aging and antiatherosclerosis properties [4,5]. Klotho gene polymorphism has been shown to be associated with the severity of renal failure and aging parameters such as common carotid artery intima media thickness, low density lipoprotein and uric acid levels in CKD patients [6][7][8] . Klotho gene mutations may cause early dysfunction and thrombosis of vascular accesses in haemodialysis patients [9].…”

Bone Health and Renal Replacement Therapies: Which are the Best?

“…Klotho geninin bazı yaş-lanmayı engelleyici ve aterosklerozu önleyici özelliklerinin olduğu bilinmketedir (8)(9)(10). Klotho gen polimorfizminin KBH hastalarında böbrek yetmezliğinin şiddeti ve karotid arter intima media kalınlığı, düşük dansiteli lipoprotein ve ürik asid düzeyleri gibi yaşlanma belirteçleriyle ilişkili olduğu gösteril-miştir (11)(12)(13). Klotho gen mutasyonları hemodiyaliz hastalarının damar giriş yollarında erken disfonksiyon ve tromboza neden olabilirler (14).…”