<i>Mycoplasma capricolum</i> subsp. <i>capripneumoniae</i>, the cause of contagious caprine pleuropneumonia, comprises two distinct biochemical groups

Abstract

Mycoplasma capricolum subsp. capripneumoniae, the cause of the World Organisation of Animal Health- listed contagious caprine pleuropneumonia, is a member of the Mycoplasma mycoides cluster which comprises five pathogenic mycoplasmas of ruminants. These mycoplasmas are closely related immunologically and genetically which can lead to difficulties for differential diagnosis. The patterns of substrate metabolism of strains of M. c. capripneumoniae, gathered from diverse geographic regions, were studied by measurement of oxygen uptake rates. The strains fell into two major biochemical groups: one which only oxidised organic acids and glycerol and the other which could additionally metabolise sugars. Furthermore when DNA-DNA hybridisation tests were carried out these two groups of strains could be separated by their degree of DNA homology, the mean hybridisation value between members of the two groups was 86% well above the value of 70% normally used to indicate separate species. DNA-DNA hybridisation was also carried out between M. c. capripneumoniae strains and other members of the M. mycoides cluster. These experiments used labelled DNA from two representative subsp. capripneumoniae strains; these were 7/1a (organic acid-oxidising) and 4/2 LC (glucose-oxidising). The results showed a particularly close relationship of the glucose-oxidising strain to M leachii strains.

Contagious caprine pleuropneumonia – a comprehensive review

“…These were well illustrated by DNA-DNA hybridization tests as different species (Soayfane et al. 2018).…”

“…2015) and biochemical groups namely organic acid-oxidizing group and glycerol and the glucose-oxidizing group (Soayfane et al. 2018) have been identified.…”

“…2006; Soayfane et al. 2018) or to its immunogenic potential, for example, surface antigens which are glycolipids and proteins, as detected by CFT and ELISA, respectively (OIE 2014; Peyraud et al. 2014), and genetic make up, specific genes or loci, for example, 16S rRNA genes (Bascunana et al.…”

“…Recently, based on oxygen uptake rates, Mccp have been divided into two major biochemical groups, namely organic acid-oxidizing group that metabolise oxidised organic acids and glycerol and the glucose-oxiding group that metabolises sugars (Soayfane et al. 2018). These were well illustrated by DNA-DNA hybridization tests as different species (Soayfane et al.…”

Novel Candidates for Vaccine Development Against Mycoplasma Capricolum Subspecies Capripneumoniae (Mccp)—Current Knowledge and Future Prospects

“…Some of the antigens recognized in mycoplasmas are pyruvate dehydrogenase [88], surface lipoproteins LppA (p72) [89], LppB, LppC, and LppQ, the surface protein Vmm [88,90,91], dihydrolipoamide acetyltransferase [88], P30, P40 [92,93], galactofuranose [94], dihydrolipoyl dehydrogenase, MbovP579 [95], phosphate acetyltransferase, substrate binding protein (OppA), permease (OppC), ATP-binding protein (OppF) [96], phosphopyruvate hydratase, variable surface membrane proteins family (Vpma) [97], adenine phopshoribosyltransferase, Pts-G (glucose phosphotransferase system permease) [98], transketolase, translation elongation factors G and Ts [88], recombinant E1 beta subunit of the pyruvate dehydrogenase complex [99], FMN-dependent NADH-azoreductase, peptide methionine sulfoxide reductase, inorganic diphosphatase, and trigger factor [88]. Further, Mccp has two distinct biochemical groups based on different metabolizing abilities for organic acid; and glucose and glycerol indicating virulence mechanism for pathogenesis [100].…”