<i>Ocimum americanum </i>L<i>.</i> Essential Oil Exhibits Antimicrobial Activity Against Oral Bacteria Related to Periodontal Disease

Abstract

The aim of this study was to evaluate the antimicrobial property of essential oil extracted from the leaves ofOcimum americanumagainst oral bacteria related to periodontal disease. Three species of periodontal pathogens includingPorphyromonas gingivalisW50,Prevotella intermediaATCC 25611 andFusobacterium nucleatumATCC 25586 were included in the study. Agar diffusion was performed initially to screen the antimicrobial activity ofO.americanumessential oil. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were then determined using the Millipore Membrane method. The result showed that in the agar diffusion, essential oil extracted fromOcimum americanumexhibited antimicrobial activity against all test bacteria with the zone of inhibition ranging from 24 to 30 mm. The MIC values againstP. gingivalisandP. intermediawere 0.35 mg/mL whereas that ofF. nucleatumwas 0.70 mg/mL. The MBC values againstP. gingivalisandP. intermediawere 0.70 mg/mL whereas that ofF. nucleatumwas 1.4 mg/mL. In conclusion,O.americanumessential oil has an antimicrobial activity which may be a beneficial component of oral health care products to control or prevent periodontal disease by reducing these bacteria in the oral cavity.

Comparative Antimicrobial Study of Different Parts of <i>Ocimum americanum</i> L., <i>O. basilicum</i> L. and <i>O. sanctum</i> L. in Comparison to Standard Antibiotics Collected from Dibrugarh, Assam

“…Thaweboon et al [50] showed that essential oils from the plant recorded antimicrobial activity against three different micro-organisms including C. albicans using biofilm model. The essential oil from leaves also recorded antibacterial activity against oral bacteria related to periodontal disease [51]. In case of O. basilicum, hot petroleum ether extract of mature leaves recorded a significant inhibition against E. faecalis and all other extracts recorded no inhibition or negligible inhibition.…”