<p>A Comparative Study of Fluoroquinolone-Resistant Escherichia coli Lineages Portrays Indistinguishable Pathogenicity- and Survivability-Associated Phenotypic Characteristics Between ST1193 and ST131</p>

Abstract

Background: Sequence type 1193 is a new such lineage among fluoroquinolone-resistant Escherichia coli, which has risen dramatically within the last several years. However, reasons for rapid emergence and successful spread of E. coli ST1193 remain unclear. The aim of this study was to compare the pathogenicity and survivability features of E. coli ST1193 with global epidemic lineage, ST131. Methods: A total of 30 E. coli were used in this study. Isolates were divided into two groups, ST1193 (n=15) and ST131 (n=15). Adhesion and invasion to T24 cells and resistance to serum were quantified and compared among two groups. Biofilm formation capacity was assessed by crystal violet assay. Macrocolony formation was assessed on macrocolony formation plates. Resistance to hydrogen peroxide was performed by broth microdilution. RAW264.7 cells were used to assess the anti-phagocytic function of different isolates. Results: Adhesion and invasion assays revealed that E. coli ST1193 could adhere and invade T24 cells (p <0.05). 93.3% of E. coli ST1193 could form biofilms. The majority of E. coli ST1193 (66.7%) possessed no curli/no cellulose on macrocolony formation plates. E. coli ST1193 showed significant growth in serum and hydrogen peroxide and illustrated higher anti-phagocytic function to RAW264.7 cells (p <0.05). Group analysis showed that E. coli ST1193 was similar to ST131 in pathogenicity-and survivability-associated phenotypic characteristics (p >0.05). Conclusion: Our study provided more insights into pathogenicity and survivability features of E. coli ST1193, which was similar to ST131. Our study could be of great importance in understanding the emergence of global spread E. coli ST1193. Strategic and continued surveillance should be carried out to prevent the infections caused by E. coli ST1193.

Antimicrobial Resistance and Molecular Epidemiology of Escherichia coli From Bloodstream Infection in Shanghai, China, 2016–2019

“…Unlike earlier studies (36), ST1193 rather than ST405 was the second most prevalent sequence type in this study. ST1193 E. coli has increased dramatically in recent years and is emerging as a new, virulent, and resistant spectrum of fluoroquinolone-resistant E. coli (37,38), and our antimicrobial sensitivity tests suggested that ST1193 had a much higher rate of quinolone resistance than the other sequence type E. coli. Furthermore, ST1193 also had a high resistant rate to cephalosporins and ampicillin and the percentage of ESBLsproducers was 80.0%.…”

“…Furthermore, ST1193 also had a high resistant rate to cephalosporins and ampicillin and the percentage of ESBLs-producers was 80.0%. It has been reported that ST1193 was similar to ST131 in pathogenicity- and survivability-associated phenotypic characteristics ( 37 ) and its increase may be related to horizontal transfer of epidemic plasmid IncI1/ST16 ( 39 ). We need to pay attention to the wide spread of ST1193 and prevent the mass clonal spread of ST1193 like ST131.…”

Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections

“…In recent years, an increase in the number of E. coli strains resistant to ciprofloxacin has been observed. According to studies, this increase is mainly due to the dissemination of international clones ST131 (Subclones 131-H30-R and 131-H30Rx) and ST1193, which have mutations in the gyrA and parC genes that confer resistance to fluoroquinolones [80].…”

Genomic and functional characterization of carbapenem-resistant Klebsiella pneumoniae from hospital wastewater

“…The serum resistance assay was performed twice in triplicates. E. coli EC505 (stored in our laboratory) was used as the positive control and E. coli DH5α was used as the negative control [ 41 , 52 ].…”