&lt;p&gt;A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression&lt;/p&gt;

Yang, Shouhang; Chen, Ming; Lin, Chuan-Fu

doi:10.2147/cmar.s231376

Cited by 17 publications

(15 citation statements)

References 22 publications

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“…There are multiple evidences indicated that miRNA is associate with pathogenesis and progression of OS, such as miR-16, miR-27a, and miR-181 (Jones et al, 2012). Excitingly, Yang et al (2019) previously verified that MYOSLID/miR-1286/RAB13…”

Section: Discussionmentioning

confidence: 99%

“…There are multiple evidences indicated that miRNA is associate with pathogenesis and progression of OS, such as miR‐16, miR‐27a, and miR‐181 (Jones et al, 2012). Excitingly, Yang et al (2019) previously verified that MYOSLID/miR‐1286/RAB13 axis promoted the OS progression. Thereby, we supposed that GANT61 may play an anticancer role through the miR‐1286/RAB13 axis.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated that differential expression patterns of microRNAs (miRNAs) are promising tools for the diagnosis and treatment of OS (Miao et al, 2013). A recent study showed that the MYOSLID/miR‐1286/RAB13 axis is a novel regulatory signaling in promoting OS progression (Yang et al, 2019). Therefore, in the present study, we aimed to investigate the antitumor effects of GANT61 on OS in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

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GANT61 plays antitumor effects by inducing oxidative stress through the miRNA‐1286/RAB31 axis in osteosarcoma

Zhang

Chu

2020

Cell Biology International

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Osteosarcoma (OS) is a rare malignancy of bone associated with poor clinical outcomes. The antitumor effects of GANT61 on OS is unclear. To investigate antitumor effects and mechanism of GANT61 in OS cells and xenograft model. Effects of GANT61 on cell viability, clone formation, cell cycle, apoptosis, migration, and invasion ability of OS cells were assessed. Reactive oxygen species (ROS) levels measured by dichlorofluorescein fluorescence were used to evaluate oxidative stress. The Xenograft model was constructed to investigate the antitumor effects of GANT61 in vivo. The microRNA (miRNA)‐1286 was downregulated, while RAB31 upregulated in OS tissues and cells. GANT61 inhibited viability, migration, and invasion ability of OS cells (SaOS‐2 and U2OS), and induced apoptosis and the ROS production, along with miRNA‐1286 upregulation and RAB13 downregulation. After knockdown of miRNA‐1286, GANT6‐induced cell inhibition was attenuated, along with RAB31 upregulation. Inversely, miRNA‐1286 overexpression downregulated RAB31. Dual‐luciferase reporter assay verified that miR‐1286 negatively targeted RAB13. Moreover, the knockdown of RAB31 stimulated apoptosis and ROS production while inhibited viability, migration, and invasion of GANT61‐treated cells. In vivo experiments further confirmed that GANT61 inhibited tumor growth and RAB13 expression, but enhanced miRNA‐1286. The study demonstrated that GANT61 inhibited cell aggressive phenotype and tumor growth by inducing oxidative stress through the miRNA‐1286/RAB31 axis. Our findings provided a potential antitumor agent for the OS clinical treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GANT61 plays antitumor effects by inducing oxidative stress through the miRNA‐1286/RAB31 axis in osteosarcoma

Zhang

Chu

2020

Cell Biology International

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“…31 Several previous reports had demonstrated the conflicting roles of miR-1286 in human carcinogenesis. 19,[32][33][34] These contradictory results might be in part due to the different types of tumors in these reports, where miR-1286 exerted an anti-tumor property in osteosarcoma 19,32 and contributed to the progression of cutaneous melanoma 33 and lung adenocarcinoma. 34 Importantly, miR-1286 expression was remarkably altered by cisplatin or 5-fluorouracil chemotherapy in esophageal cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“… 18 When we used the online algorithm to identify the targeted miRNAs of circHIPK3, we found that circHIPK3 harbored a putative complementary site for miR-1286, an anti-tumor miRNA in osteosarcoma. 19 However, it is still unexplored whether the regulation of circHIPK3 on BC PTX resistance is mediated by miR-1286.…”

Section: Introductionmentioning

confidence: 99%

Silencing of circHIPK3 Sensitizes Paclitaxel-Resistant Breast Cancer Cells to Chemotherapy by Regulating HK2 Through Targeting miR-1286

Ni¹,

Xi²,

Xiao³

et al. 2021

CMAR

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Background: Resistance development to paclitaxel (PTX) has become a major obstacle in the successful treatment of breast cancer (BC). Circular RNAs (circRNAs) have been identified as essential regulators in PTX resistance of BC. Here, we explored the precise roles of circRNA homeodomain interacting protein kinase 3 (circHIPK3, circ_0000284) in PTX resistance of BC. Methods: The expression levels of circHIPK3, microRNA (miR)-1286, and hexokinase 2 (HK2) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Ribonuclease R (RNase R) assay was used to confirm the stability of circHIPK3. Cellular localization of circHIPK3 was assessed by subcellular localization assay. The half maximal inhibitory concentration (IC 50 ) value for PTX was measured by Cell Counting Kit-8 (CCK-8) assay. Cell colony formation, cell cycle distribution, and apoptosis were gauged by colony formation assay and flow cytometry, respectively. Animal studies were performed to evaluate the role of circHIPK3 in vivo. The direct relationship between miR-1286 and circHIPK3 or HK2 was verified by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Results: Our results showed that circHIPK3 was up-regulated in PTX-resistant BC tissues and cells compared with the sensitive counterparts. The silencing of circHIPK3 promoted PTX sensitivity of PTX-resistant BC cells in vitro and in vivo. CircHIPK3 directly targeted miR-1286, and miR-1286 acted as a downstream mediator of circHIPK3 function in vitro. HK2 was a direct target of miR-1286, and circHIPK3 modulated HK2 expression through miR-1286. The increased expression of miR-1286 sensitized PTX-resistant BC cells to PTX in vitro by down-regulating HK2. Conclusion:Our findings demonstrated that the silencing of circHIPK3 sensitized PTXresistant BC cells to PTX therapy at least in part via the regulation of the miR-1286/HK2 axis.

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lncRNA BZRAP1‐AS1 alleviates rheumatoid arthritis by regulating miR‐1286/COL5A2 axis

Zhu

2021

Immunity Inflam & Disease

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Background: Dysregulation of BZRAP1-AS1 was associated with immune statuses of cancer or Alzheimer's disease patients, yet little is known about its role in rheumatoid arthritis.Methods: RT-qPCR and western blot were applied to assess the expression of indicated expression. CCK-8 and BrdU proliferation assays were used to measure the proliferation of RA-HFLS. Apoptosis in RA-HFLS was evidenced by the alteration of caspase-3 activity and apoptosis-related factors. ELISA was performed to detect IL-6, IL-1β, and TNF-α level. Luciferase reporter, RIP, and pull-down assays were used to confirm the BZRAP1-AS1/miR-1286/COL5A2 cascade predicted by bioinformatics analysis.Results: BZRAP1-AS1 and COL5A2 were downregulated in RA tissues and RA-HFLS while miR-1286 was amplified. Overexpression of BZRAP1-AS1 reduced the RA-HFLS proliferation, IL-6, IL-1β, and TNF-α level and induced cell apoptosis while BZRAP1-AS1 silence produced an opposite effect. Overexpression of BZRAP1-AS1 reduced the miR-1286 expression which in turn increased the COL5A2 expression, thereby relieving the excessive proliferation and limited apoptosis in RA-HFLS. Conclusion:Our findings suggested that BZRAP1-AS1 sequestered miR-1286 and reshaped the COL5A2 expression, thereby suppressed RA-HFLS proliferation and inflammation, and triggered cell apoptosis, resulting in the attenuation of RA progression.

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<p>A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression</p>

Cited by 17 publications

References 22 publications

GANT61 plays antitumor effects by inducing oxidative stress through the miRNA‐1286/RAB31 axis in osteosarcoma

GANT61 plays antitumor effects by inducing oxidative stress through the miRNA‐1286/RAB31 axis in osteosarcoma

Silencing of circHIPK3 Sensitizes Paclitaxel-Resistant Breast Cancer Cells to Chemotherapy by Regulating HK2 Through Targeting miR-1286

lncRNA BZRAP1‐AS1 alleviates rheumatoid arthritis by regulating miR‐1286/COL5A2 axis

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