&lt;p&gt;Anti-Proliferative and Apoptosis-Promoting Effect of microRNA-125b on Pancreatic Cancer by Targeting NEDD9 via PI3K/AKT Signaling&lt;/p&gt;

Xue, Yuzheng; Yao, Zhong; Wu, Tielong; Sheng, Yingyue; Dai, Yuanyuan; Xu, Lingling; Bao, Chuanqing

doi:10.2147/cmar.s227315

Cited by 10 publications

(12 citation statements)

References 29 publications

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“…Therefore, controlling the metastasis is important for the treatment of BC [ 32 , 33 ]. Various studies have shown that NEDD9 is highly expressed in malignant tumour tissues, such as glioma, colorectal and liver cancer tissues, and it has been suggested that it plays an important role in tumour invasion, metastasis and migration [ 34 , 35 ]. NEDD9 is a molecule related to tumour invasion and metastasis [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…Various studies have shown that NEDD9 is highly expressed in malignant tumour tissues, such as glioma, colorectal and liver cancer tissues, and it has been suggested that it plays an important role in tumour invasion, metastasis and migration [ 34 , 35 ]. NEDD9 is a molecule related to tumour invasion and metastasis [ 34 , 35 ]. There have been several reports about the involvement of NEDD9 in the development of BC [ 36 , 37 ], but the precise molecular mechanism of NEDD9 in BC metastasis remains unclear.…”

Section: Discussionmentioning

confidence: 99%

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miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

et al. 2022

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Background As a metastasis-related protein, NEDD9 has been reported in breast cancer (BC) metastasis research. However, there are few studies on the upstream regulators of NEDD9, especially involving the potential role of miRNAs. The purpose of this study was to explain whether miR-107 potentially regulates NEDD9, which may lead to invasion and metastasis of BC. Methods MCF-7 and MDA-MB-231 cells were transduced with lentiviruses to construct stably transduced cells with miR-107 overexpression, miR-107 silencing or empty vectors. A luciferase reporter assay was performed to verify the binding of miR-107 and NEDD9. The scratch test and Transwell assay were used to measure cell migration and invasion ability, respectively. For the study of metastasis in vivo, we injected MDA-MB-231 cells into the fat pad of nude mice to develop an orthotopic breast cancer model. Results We found that NEDD9 expression correlates with the prognosis of BC patients. In BC cell lines, NEDD9 was positively correlated with cell migration ability. Further research revealed that miR-107 inhibited NEDD9 expression by targeting the 3′-untranslated region of NEDD9. Overexpression of miR-107 suppressed the expression of NEDD9, thereby inhibiting the invasion, migration and proliferation of BC cells, but interference with miR-107 promoted the expression of NEDD9 as well as invasion, migration and proliferation. In an in vivo model, overexpression of miR-107 decreased the expression of NEDD9 and inhibited tumour growth, invasion and metastasis; however, these effects were reversed by inhibiting miR-107. Conclusions These findings indicated the potential role of miR-107 in regulating NEDD9 in the invasion, migration and proliferation of BC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

et al. 2022

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“…Wang et al (21) showed that upregulation of glutathione Stransferase mu-3 significantly inhibited cell proliferation by delaying the G0/G1 phase transition. Xue et al (22) observed that miR-125b could be downregulated in pancreatic ductal adenocarcinoma. On the contrary, upregulation of miR-125b could arrest the cells in the G0/G1 phase.…”

Section: Discussionmentioning

confidence: 99%

β-Hydroxyisovaleryl-Shikonin Exerts an Antitumor Effect on Pancreatic Cancer Through the PI3K/AKT Signaling Pathway

et al. 2022

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Pancreatic cancer (PC) is marked with a low survival rate and lack of recognized effective treatment strategy. We investigated the antitumor effect of β-hydroxyisovaleryl-shikonin (β-HIVS) on PC and the associated working mechanism. Cell toxicity was determined using Cell Counting Kit-8 (CCK-8) assay. Acridine Orange/Ethidium Bromide (AO/EB) double-fluorescent staining assay accompanied by flow cytometry was utilized to estimate cell apoptosis. Cell cycle, reactive oxygen species (ROS), and mitochondrial membrane potential were all evaluated using flow cytometry. Transwell and wound healing assays were performed to evaluate cell migration and invasion. Protein expression was analyzed by Western blots. A xenograft mouse model was employed to determine the antitumor effect of β-HIVS in vivo. PC cell viability gradually decreased with increasing β-HIVS while apoptosis was enhanced together with cell-cycle blockage in the G0–G1 phases. β-HIVS induced mitochondrial dysfunction, ROS production, and DNA damage and inhibited the invasive and migratory ability of PC cells. We further confirmed the suppression of EMT and PI3K/AKT pathways as underlying mechanisms. The mouse model treated with the increasing dose of β-HIVS displayed decreased tumor growth rate, along with increased apoptosis. Thus, β-HIVS exerts antitumor effects on PC through inducing apoptosis, ROS production, decreasing mitochondrial membrane potential, and suppressing signal pathways, such as PI3K/AKT. In summary, β-HIVS might be a promising strategy for PC treatment.

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“… 40 , 41 , 42 , 43 , 44 Additionally, NEDD9 expression negatively associated with miR ‐ 125a / b was shown in pancreatic cancer, lung adenocarcinoma and melanoma. 45 , 46 , 47 In pancreatic cancer and hepatocellular carcinoma, NEDD9 was reported to be regulated by miR ‐ 18a playing a key role during carcinogenesis. 48 , 49 In our study, we for the first time found that NEDD9 expression was negatively associated with miR ‐ 381 in AML.…”

Section: Discussionmentioning

confidence: 99%

NEDD9 overexpression: Prognostic and guidance value in acute myeloid leukaemia

Hua

Feng

Yin

et al. 2021

J Cellular Molecular Medi

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Acute myeloid leukaemia (AML) is a blood cancer characterized by clonal myeloid precursors in bone marrow (BM), leading to haematopoiesis failure. 1 Clinical outcome of AML is highly heterogeneous, survive time from days to cure. 1 Cytogenetic abnormalities and gene mutations obtained at the diagnosis time provide the most important information. 2 Recently, aberrant gene expression has also been found to be associated with prognosis in AML, such as BAALC, MN1, ERG, and WT1. 3 Therefore, identification of newly developed biomarkers and construction of molecular-based prognostic risk scores could more precisely recognize the patients who are at high risk, and finally give intensive treatment to improve their clinical outcome.

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<p>Anti-Proliferative and Apoptosis-Promoting Effect of microRNA-125b on Pancreatic Cancer by Targeting NEDD9 via PI3K/AKT Signaling</p>

Cited by 10 publications

References 29 publications

miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

β-Hydroxyisovaleryl-Shikonin Exerts an Antitumor Effect on Pancreatic Cancer Through the PI3K/AKT Signaling Pathway

NEDD9 overexpression: Prognostic and guidance value in acute myeloid leukaemia

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