<p>Assessments of Amino Acids, Ammonia and Oxidative Stress Among Cohort of Egyptian Autistic Children: Correlations with Electroencephalogram and Disease Severity</p>

Abstract

Objective: The current study aimed to assess the profiles of plasma amino acids, serum ammonia and oxidative stress status among autistic children in terms of electroencephalogram findings and clinical severity among the cohort of autistic Egyptian children. Patients and methods: The present study included 118 Egyptian children categorized into 54 children with autism who were comparable with 64 healthy controls. Clinical assessments of cases were performed using CARS in addition to EEG records. Plasma amino acids were measured using high-performance liquid chromatography (HPLC), while, serum ammonia and oxidative stress markers were measured using colorimetric methods for all included children. Results: The overall results revealed that 37.04% of cases had abnormal EEG findings. Amino acid profile in autistic children showed statistically significant lower levels of aspartic acid, glycine, β-alanine, tryptophan, lysine and proline amino acids with significantly higher asparagine amino acid derivative levels among autistic patients versus the control group (p˂0.05). There were significantly higher serum ammonia levels with significantly higher total oxidant status (TOS) and oxidative stress index (OSI) values among the included autistic children vs controls (p˂0.05). There were significantly negative correlations between CARS with aspartic acid (r=−0.269, P=0.049), arginine (r=-0.286, p= 0.036), and TAS (r= −0.341, p= 0.012), and significantly positive correlations between CARS with TOS (r=0.360, p= 0.007) and OSI (r= 0.338, p= 0.013). Conclusion: Dysregulated amino acid metabolism, high ammonia and oxidative stress were prevalent among autistic children and should be considered in autism management. Still EEG records were inconclusive among autistic children, although may be helpful in assessment autism severity.

Protective Effect of Lactoferrin against Chromium Induced Adverse Renal Changes in Rats: Oxidative Stress Theory

“…318 001, 253 001 and 253 001 respectively. While, TAO kit was supplied by Biodiagnostics, Egypt (Saleem et al, 2018;Hassan et al, 2019;Saleem et al, 2020).…”

1H-NMR-Based Metabolomics in Autism Spectrum Disorder and Pediatric Acute-Onset Neuropsychiatric Syndrome

“…An asparagine deficit in both ASD and PANS could lead to detrimental effects on inflammatory homeostasis, suggesting its potential “inflammatory signature” for both groups. It should be considered, however, that the increase in serum asparagine in ASD patients has also been reported [ 49 , 50 ]; distinct ethnic backgrounds of the patients or different measurement methodologies (i.e., HPLC) could explain these differences.…”

Serum Levels of Growth-Associated Protein-43 and Neurotrophin-3 in Childhood Epilepsy and Their Relation to Zinc Levels

“…For analysis, a minimum of 75 s of artifact-free EEG was provided. The data was divided into 4-s epochs after artifacts were removed, and the magnitude of each frequency band in microvolts was determined using Fourier power spectrum analysis [ 21 ].…”