<p>Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status</p>

Abstract

Background MicroRNAs (miRNAs) miRNA-499 rs3746444 A>G polymorphism may be complicated in the susceptibility to cancer. However, the correlation of this polymorphism with adenocarcinoma of esophagogastric junction (AEG) was unknown. Patients and methods A total of 1063 AEG patients and 1677 controls were included in this study to assess the association of miR-499 rs3746444 A>G with AEG risk. SNPscan TM genotyping assay was harnessed to obtain the genotypes of miRNA-499 rs3746444 A>G polymorphism. Results We identified that SNP miR-499 rs3746444 A>G increased the susceptibility of AEG (AG vs AA: adjusted OR=1.25, 95% CI=1.05–1.49, P =0.012 and AG/GG vs AA: adjusted OR=1.30, 95% CI=1.10–1.54, P =0.002). In a stratified analysis, we found that miR-499 rs3746444 A>G polymorphism had an increased susceptibility of AEG in several subgroups (male subgroup: AG vs AA: adjusted P =0.004 and AG/GG vs AA: adjusted P =0.002; female subgroup: GG vs AG/AA: adjusted P =0.046; <64 years subgroup: AG vs AA: adjusted P =0.006 and AG/GG vs AA: adjusted P =0.003; never smoking subgroup: AG vs AA: adjusted P =0.003 and AG/GG vs AA: adjusted P =0.001; and never drinking subgroup: AG vs AA: adjusted P =0.008 and AG/GG vs AA: adjusted P =0.002). The results of power calculation indicated that miR-499 rs3746444 A>G polymorphism increased the risk of AEG in overall comparison, male, <64 years, never smoking, and never drinking subgroups. Among the AEG cases, 625 patients accompanied by positive lymph node. However, the distribution of miRNA-499 rs3746444 A>G variants was no significant difference between different lymph node status. Conclusion Our findings indicate that miR-499 rs3746444 A>G polymorphism is significantly associated with AEG susceptibility. In the future, further exploration of this genetic factor in relation to AEG susceptibility with an adequate methodological quality is needed.

Investigation of BTLA tagging variants with risk of esophagogastric junction adenocarcinoma

“…All statistical analyses described previously were performed in SAS 9.4 software (SAS Institute Inc., Cary, NC, U.S.A.). Using PS software (http://biostat.mc.vanderbilt.edu/twiki/bin/view/ Main/PowerSampleSize), the power value (α = 0.05) was calculated [27,28]. We also used the false-positive report probability (FPRP) to determine the significant findings [29].…”

“…Using PS software (http://biostat.mc.vanderbilt.edu/twiki/bin/view/Main/PowerSampleSize), the power value (α = 0.05) was calculated [27,28]. For BTLA rs1982809 G>A SNP, the power value was 0.631 in AA versus GG genetic model and 0.589 in AA versus GG/GA genetic model among ever smokers.…”

Association of rs3746444 in MiRNA-499 and rs3787016 in Long Non-coding RNAs POLR2E with Prostate Cancer: an updated meta-analysis and systematic review

“…MicroRNA-499 rs3746444 has been proved to be signi cantly associated with increased risk of cancer of the respiratory system, digestive system,urinary system and gynecological system [42][43][44][45][46][47][48][49][50][51][52][53].Existing studies have been veri ed that the MiRNA-499(rs3746444 T > C) gene polymorphism can control the expression level of SOX gene. Due to the up-regulation of SOX6 gene, the anti-apoptosis function of MiRNA-499(rs3746444 T > C) can be reversed [54].…”

Methylenetetrahydrofolate reductase C677T (Ala&gt;Val, rs1801133 C&gt;T) polymorphism decreases the susceptibility of hepatocellular carcinoma: a meta-analysis involving 12,628 subjects

“…By omitting a study one by one and analyzing the remainders, sensitivity analysis was performed to assess the stability of our findings. The distribution of the MTHFR rs1801133 genotype was used to calculate the P value of HWE by using an online software (http://ihg.gsf.de/cgi-bin/hw/hwa1.pl) in controls [33][34][35]. STATA 12.0 software (Stata Corp., College Station, Texas) was used to conduct the analysis.…”