&lt;p&gt;Cerebrospinal Fluid Proteomics For Identification Of α2-Macroglobulin As A Potential Biomarker To Monitor Pharmacological Therapeutic Efficacy In Dopamine Dictated Disease States Of Parkinson’s Disease And Schizophrenia&lt;/p&gt;

Gupta, Ashish Kumar; Pokhriyal, Ruchika; Khan, Mohd Imran; Kumar, Domada Ratna; Gupta, Rishab; Chadda, Rakesh Kumar; Ramachandran, Rashmi; Goyal, Vinay; Tripathi, Manjari; Hariprasad, Gururao

doi:10.2147/ndt.s214217

Cited by 15 publications

(8 citation statements)

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“…Each experiment composed of at least one SLNM + , one SLNM- and, one of either of these two phenotypes or a cancer control benign breast tumor tissue. The equimolar culmination of the three phenotypes was made into an internal standard for the sake of normalization for each of the four experiments 27 . 80 μg of protein from each phenotype sample was reduced with 25 mM DTT for 30 min at 60 °C and alkylated with 55 mM iodoacetamide for 20 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…In-house protocols were used for Mass spectrometry data acquisition 27 . Peptides from the 44 fractions were desalted and concentrated using reversed phase ZipTip, and reconstituted in 0.1% formic acid.…”

Section: Methodsmentioning

confidence: 99%

“…Our team has been identifying potential protein biomarkers in various clinical phenotypes in the last few years 27 , 28 . In the recent past, we have carried out a gel based proteomics experiment to identify biomarkers that can flag lymph node metastasis in early breast cancer 29 .…”

Section: Introductionmentioning

confidence: 99%

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iTRAQ proteomics of sentinel lymph nodes for identification of extracellular matrix proteins to flag metastasis in early breast cancer

Pathania

Khan

Bandyopadhyay

et al. 2022

Sci Rep

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Patients with early breast cancer are affected by metastasis to axillary lymph nodes. Metastasis to these nodes is crucial for staging and quality of surgery. Sentinel Lymph Node Biopsy that is currently used to assess lymph node metastasis is not effective. This necessitates identification of biomarkers that can flag metastasis. Early stage breast cancer patients were recruited. Surgical resection of breast was followed by identification of sentinel lymph nodes. Fresh frozen section biopsy was used to assign metastatic and non-metastatic sentinel lymph nodes. Discovery phase included iTRAQ proteomics coupled with mass spectrometric analysis to identify differentially expressed proteins. Data is available via ProteomeXchange with identifier PXD027668. Validation was done by bioinformatic analysis and ELISA. There were 2398 unique protein groups and 109 differentially expressed proteins comparing metastatic and non-metastatic lymph nodes. Forty nine proteins were up-regulated, and sixty proteins that were down regulated in metastatic group. Bioinformatic analysis showed ECM-receptor interaction pathways to be implicated in lymph node metastasis. ELISA confirmed up-regulation of ECM proteins in metastatic lymph nodes. ECM proteins have requisite parameters to be developed as a diagnostic tool to assess status of sentinel lymph nodes to guide surgical intervention in early breast cancer.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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iTRAQ proteomics of sentinel lymph nodes for identification of extracellular matrix proteins to flag metastasis in early breast cancer

Pathania

Khan

Bandyopadhyay

et al. 2022

Sci Rep

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“…Moreover, the gene encoding this protein is considered as a PD risk gene [ 25 , 26 , 27 ]. It is noteworthy that in PD, the level of α-2-macroglobulin in the CSF changes, which is considered as a diagnostic marker [ 24 , 28 , 29 ]. Based on these data, one of the objectives of this study was to assess for the first time the activity of α-2-macroglobulin in the TF in PD patients.…”

Section: Introductionmentioning

confidence: 99%

A Pilot Study of Changes in the Level of Catecholamines and the Activity of α-2-Macroglobulin in the Tear Fluid of Patients with Parkinson’s Disease and Parkinsonian Mice

Bogdanov

Kim

Nodel

et al. 2021

IJMS

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Development of differential and early (preclinical) diagnostics of Parkinson’s disease (PD) is among the priorities in neuroscience. We searched for changes in the level of catecholamines and α-2-macroglobulin activity in the tear fluid (TF) in PD patients at an early clinical stage. It was shown that TF in patients is characterized by an increased level of noradrenaline mainly on the ipsilateral side of pronounced motor symptoms (72%, p = 0.049), a decreased level of adrenaline on both sides (ipsilateral—53%, p = 0.004; contralateral—42%, p = 0.02), and an increased α-2-macroglobulin activity on both sides (ipsilateral—53%, p = 0.03; contralateral—56%, p = 0.037) compared to controls. These changes are considered as potential biomarkers for differential diagnosis. Similar changes in the TF were found in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice when modeling clinical and preclinical stages of PD. These data show the adequacy of models to the pathogenesis of PD along the selected metabolic pathways, and also suggest that the found TF changes can be considered as potential biomarkers for preclinical diagnosis of PD. In Parkinsonian mice, the level of catecholamines also changes in the lacrimal glands, which makes it possible to consider them as one of the sources of catecholamines in the TF.

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“…In the recent past, our team has been actively involved in identifying potential protein biomarkers to understand various clinical phenotypes. [28][29][30] In this study, a 2-D fluorescence difference gel electrophoresis (DIGE) experiment has been carried out using sentinel lymph node tissue to identify a biomarker that can accurately reflect the metastatic lymph node in early breast cancer.…”

Section: Introductionmentioning

confidence: 99%

<p>Proteomics of Sentinel Lymph Nodes in Early Breast Cancer for Identification of Thymidylate Synthase as a Potential Biomarker to Flag Metastasis: A Preliminary Study</p>

Pathania

Khan

Kumar

et al. 2020

CMAR

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Introduction: Breast cancer is the second most common cancer in women across the world. Some of the patients who present in the early stage of disease are affected by metastasis to the axillary group of lymph nodes. The first among this group that is affected is called as sentinel lymph node, and its diagnosis is crucial for the staging of cancer thereby dictating the type of surgical therapy. Therefore, the sentinel lymph node status provides the most relevant information to the surgeon and patient prognosis. The expanded utilization of breast conservation surgery has declined the morbidity associated with mastectomy and axillary lymph node surgery. Recent interest is, therefore, centered on techniques that allow accurate assessment of the sentinel lymph node metastasis. A current procedure such as sentinel lymph node biopsy (SLNB) that is used to assess axillary lymph node metastasis is neither specific nor sensitive, and besides, it is time-consuming. Objective: To compare the protein profiles between metastatic and non-metastatic lymph nodes to identify a biomarker that can flag lymph node metastasis. Materials and Methods: Women with early breast cancer were screened using mammography imaging and recruited to the study. Surgical resection was done to remove the breast tissue, and sentinel lymph node was identified using fluorescein and methylene blue tracer. Lymph node was sliced, and one set was sent for histopathology, which was considered the gold standard to assess the metastatic status of the lymph node. One set of slices was taken for proteomic experiments. Proteins were labelled with fluorescent cyanine tags and were subjected to difference gel electrophoresis experiment. Differentially expressed spots that had at least a twofold relative ratio and consistent pattern across three sets of biological replicate experiments were marked. Gel spots were trypsin digested and identified on mass spectrometry machine. Validation study was done by Western blot experiment on the same set of samples. Results: Thymidylate synthase has a twofold higher expression in the metastatic sentinel lymph nodes as compared to non-metastatic lymph nodes in early breast cancer patients. Conclusion: Differential in gel expression proteomics is an ideal platform for the identification of potential protein biomarker candidates that can differentiate metastatic from nonmetastatic lymph nodes in early breast cancer. The identification of thymidylate synthase offers a scope to develop an on-table diagnostic kit to assess the status of sentinel lymph nodes during mastectomy procedure to guide surgical management of axillary lymph nodes in early breast cancer.

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<p>Cerebrospinal Fluid Proteomics For Identification Of α2-Macroglobulin As A Potential Biomarker To Monitor Pharmacological Therapeutic Efficacy In Dopamine Dictated Disease States Of Parkinson’s Disease And Schizophrenia</p>

Cited by 15 publications

References 92 publications

iTRAQ proteomics of sentinel lymph nodes for identification of extracellular matrix proteins to flag metastasis in early breast cancer

iTRAQ proteomics of sentinel lymph nodes for identification of extracellular matrix proteins to flag metastasis in early breast cancer

A Pilot Study of Changes in the Level of Catecholamines and the Activity of α-2-Macroglobulin in the Tear Fluid of Patients with Parkinson’s Disease and Parkinsonian Mice

<p>Proteomics of Sentinel Lymph Nodes in Early Breast Cancer for Identification of Thymidylate Synthase as a Potential Biomarker to Flag Metastasis: A Preliminary Study</p>

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