&lt;p&gt;Circular RNA circNHSL1 Contributes to Gastric Cancer Progression Through the miR-149-5p/YWHAZ Axis&lt;/p&gt;

Hui, Chunying; Tian, Li; He, Xinling

doi:10.2147/cmar.s253152

Cited by 24 publications

(19 citation statements)

References 39 publications

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“…We next demonstrated that miR-149-5p directly bound to CCDC6 or circDNA2 via dual-luciferase reporter gene assays and functional gain and loss experiments. Several studies 20 , 34 , 35 independently reported that miR-149-5p has a tumor-suppressive role in GC, which was consistent with our findings. In our study, the AUC for a single miR-149-5p expression level to predict the risk of GC occurrence reached 0.7102, indicating that miR-149-5p has potential as a biomarker for GC diagnosis.…”

Section: Discussionsupporting

confidence: 93%

Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression

Jin

Huang

Peng

et al. 2021

Molecular Therapy - Nucleic Acids

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Circular (circ)RNAs are widely involved in gastric cancer (GC) pathogenesis, and coiled-coil domain containing 6 ( CCDC6 ) is a fused partner of multiple oncogenes; however, the underlying mechanisms of how circRNAs regulate CCDC6 expression in the progression and prognosis of GC remain unclear. Here, we discovered the circRNA derived from the DNA2 gene locus (circDNA2) through RNA sequencing. By performing quantitative real-time PCR and fluorescence in situ hybridization (FISH) assays with a human tissue microarray, circDNA2 was found to be highly expressed in GC tissues and associated with lymphatic invasion of GC patients. Knockdown of circDNA2 expression suppressed the proliferation of GC cells by reducing CCDC6 expression. Mechanistically, circDNA2 acted as a microRNA (miR)-149-5p sponge, which was confirmed to target CCDC6 by RNA pulldown and dual-luciferase reporter assays and rescue experiments. Both low miR-149-5p expression and high CCDC6 expression were related to unfavorable prognosis in GC patients. Moreover, GC patients with low miR-149-5p expression had shorter overall survival and a higher risk of chemotherapy resistance than those with high miR-149-5p expression. In summary, circDNA2 contributes to the growth and lymphatic metastasis of GC by upregulating CCDC6 expression by sponging miR-149-5p. The circDNA2/miR-149-5p/ CCDC6 axis might be developed as a therapeutic target and prognostic indicator for GC.

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Section: Discussionsupporting

confidence: 93%

Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression

Jin

Huang

Peng

et al. 2021

Molecular Therapy - Nucleic Acids

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“…Studies have shown that circ RNA plays an important role in the occurrence and development of a variety of malignant tumors. 22 – 25 For example, circVAPA is highly expressed in colon cancer, 26 breast cancer 27 and liver cancer. 28 As an oncogene, circVAPA promotes the progression of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

circVAPA promotes the proliferation, migration and invasion of oral cancer cells through the miR-132/HOXA7 axis

Chen

Zhang

et al. 2021

J Int Med Res

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Objective To study the relationship between the circular RNA vesicle-associated membrane protein-associated protein A (circVAPA) and the pathogenesis of oral squamous cell carcinoma. Methods The expression of circVAPA was detected by RT-qPCR. In vitro loss-of-function experiments were performed in Cal-27 cells. The malignant phenotype of cells was evaluated by cell counting kit-8, clone formation and transwell assays. Luciferase reporter assays were used to assess the circVAPA/miR-132/homeobox A (HOXA) regulatory axis. Results circVAPA expression was significantly increased in oral cancer tissues and cells. The overall survival and progression-free survival of patients with oral cancer who exhibited high circVAPA expression were significantly shorter compared with those with low expression. circVAPA expression was closely related to tumor size, TNM stage and distant metastasis. circVAPA knockdown reduced the proliferation, invasion and migration of Cal-27 cells. MiR-132 was identified as a target of circVAPA in Cal-27 cells. Cotransfection with si-circVAPA and miR-132 inhibitor reversed the inhibitory effect of circVAPA knockdown on cell malignant phenotypes. HOXA7 was further identified as a downstream target of miR-132. Conclusion circVAPA is highly expressed in oral cancer, and its abnormal expression might affect the proliferation, invasion and migration of oral cancer cells by modulating the miR-132/HOXA7 signaling axis.

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“…Moreover, another study demonstrated that lncRNA BLACAT1 promotes the proliferation, migration and invasion of GC cells by regulating the miR-149-5p/KIF2A axis (50). CircNHSL1 can affect the expression of YWHAZ through sponging miR-149-5p, thereby regulating the progression of GC (51). Circ_0044516 could promote the expression of HuR through sponging miR-149-5p, thereby regulating the progression of GC (52).…”

Section: Gastric Cancermentioning

confidence: 99%

MiR-149-5p: An Important miRNA Regulated by Competing Endogenous RNAs in Diverse Human Cancers

et al. 2021

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MicroRNAs (miRNAs) consist of a large family of small, non-coding RNAs with the ability to result in gene silencing post-transcriptionally. With recent advances in research technology over the past several years, the physiological and pathological potentials of miRNAs have been gradually uncovered. MiR-149-5p, a conserved miRNA, was found to regulate physiological processes, such as inflammatory response, adipogenesis and cell proliferation. Notably, increasing studies indicate miR-149-5p may act as an important regulator in solid tumors, especially cancers in reproductive system and digestive system. It has been acknowledged that miR-149-5p can function as an oncogene or tumor suppressor in different cancers, which is achieved by controlling a variety of genes expression and adjusting downstream signaling pathway. Moreover, the levels of miR-149-5p are influenced by several newly discovered long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs). However, there is blank about systematic function and mechanism of miR-149-5p in human cancers. In this review, we firstly summarize the present comprehension of miR-149-5p at the molecular level, its vital role in tumor initiation and progression, as well as its potential roles in monitoring diverse reproductive and digestive malignancies.

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<p>Circular RNA circNHSL1 Contributes to Gastric Cancer Progression Through the miR-149-5p/YWHAZ Axis</p>

Cited by 24 publications

References 39 publications

Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression

Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression

circVAPA promotes the proliferation, migration and invasion of oral cancer cells through the miR-132/HOXA7 axis

MiR-149-5p: An Important miRNA Regulated by Competing Endogenous RNAs in Diverse Human Cancers

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