<p>Downregulation of circRNA_0000285 Suppresses Cervical Cancer Development by Regulating miR197-3p–ELK1 Axis</p>

Abstract

Background Circular RNAs (circRNAs) are involved in the development of human cancers, including cervical cancer (CC). However, the role and mechanism of the circRNA hsa_circ_0000285 ( circ_0000285 ) in CC development remain largely unknown. Methods Thirty paired CC and adjacent normal tissue samples were harvested. CC cell lines SiHa and HeLa were cultured in this study. The expression of circ_0000285 , miR197-3p and ELK1 was detected via qRT-PCR or Western blot. CC development was assessed via cell viability, colony formation, apoptosis, cell cycle, and autophagy using MTT, colony-formation assays, flow cytometry and Western blot. The target association was analyzed via dual luciferase–reporter assay, RNA immunoprecipitation, and RNA pull-down. The role of circ_0000285 in CC in vivo was analyzed using a xenograft model. Results circ_0000285 abundance was enhanced in CC tissue and cells and mainly located in cytoplasm. Silence of circ_0000285 suppressed cell viability and colony formation, arrested the cell cycle at the G 0 /G 1 phase, and induced apoptosis and autophagy in CC cells. miR197-3p was targeted by circ_0000285 , and miR197-3p knockdown reversed the effect of circ_0000285 silence on CC development. miR197-3p directly targeted ELK1 to inhibit CC development. circ_0000285 regulated ELK1 by modulating miR197-3p . Knockdown of circ_0000285 reduced xenograft tumor growth in vivo. Conclusion Knockdown of circ_0000285 repressed CC development by increasing miR197-3p and decreasing ELK1 .

Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA‑654‑3p

“…Previous studies have reported that circRNAs are involved in cancer development by regulating the expression of specific target genes ( 8 , 11 ). Initially, bioinformatics analysis was performed to predict the target gene of circRNA_0000285.…”

“…Previous studies have reported that circRNAs contain several miRNA binding sites, which are involved in the regulation of gene expressions by sponging miRNAs ( 9 , 10 ). circRNA_0000285 has been identified as a novel circRNA, which promotes the metastasis and invasion of several types of cancer by targeting specific miRNAs ( 11 ). However, the roles and underlying molecular mechanism of circRNA_0000285 in thyroid cancer remain unclear.…”

The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

“…It is currently theorized that circRNAs are mainly distributed in the cytoplasm and play a role in regulating tumor progression through the adsorption of miRNAs. For example, circ_0001785 can sponge miR-942 or miR-1200 to regulate breast cancer or osteosarcoma progression, respectively ( 31 , 32 ); and circ_0000285 can absorb miR-409-3p, miR-197-3p and miR-599, thereby affecting the progression of cervical cancer and osteosarcoma ( 24 , 33 , 34 ). Analysis using an online database revealed 29 miRNAs that may serve as the target of circ_0001367.…”

Identification of potential biomarkers and therapeutic targets in the chronic obstructive pulmonary disease and acute myocardial infarction

“…We found that ELK1, ETV4, STAT3 and TFAP2A were signi cant TFs, which interacted with the hub genes. TF ELK1 has been found to act a carcinogenic role in human cancers, like hepatocellular carcinoma, thyroid cancer, breast cancer [67]. Jennifer T Cairns etal.…”