<p>Effect of umbelliferone on adjuvant-induced arthritis in rats by MAPK/NF-&kappa;B pathway</p>

Abstract

Purpose Umbelliferone (Umb), a member of coumarin family, is found in many plants and is a promising molecule with potential anti-inflammatory, anti-oxidative, and anti-tumor activities. However, the effect of Umb on arthritis remains unclear. Methods A rat model with Freund’s complete adjuvant (FCA)-induced arthritis was developed and used to test the efficacy of Umb on arthritis rats. Rats were given an intragastric injection of Umb (20 and 40 mg/kg) once daily from days 21 to 28 after the administration of FCA. Hind paw volume was assessed using a volume meter. The pro-inflammatory cytokine levels and prostaglandin E2 (PEG2) level in serum and synovial fluid were detected by ELISA. HE staining was used to determine representative histological changes in joint tissues, and Western blot analysis was employed to study the effects of Umb on MAPK/NF-κB signaling pathway. Results Our results showed that Umb suppressed the release of IL-6, IL-1β, tumor necrosis factor-alpha, and PEG2. In addition, Umb could also dramatically ameliorate the pathological changes observed in rat joints. Based on the results of Western blot, we also observed that Umb could strikingly suppress the expression of MAPK/NF-κB pathway molecules. Conclusion These results proved that treatment with Umb is very effective for arthritis and inhibiting the MAPK/NF-κB signaling pathway may be a potential therapeutic target for treatment of arthritis.

Coumarins as Modulators of the Keap1/Nrf2/ARE Signaling Pathway

“…The Umbelliferae family is inclusive of economically important herbs, such as celery, cumin, fennel, parsley alexanders, angelica, asafoetida, and giant hogweed [9,170]. UMB possesses a variety of bioactivities, and several investigators have previously reported its in vivo antioxidant and anti-inflammatory effects and studied the underlying mechanisms of action in several animal models [30,[171][172][173][174][175]. The anticancer activity of UMB in hepatocellular [176], colon [177], and oral [178] carcinomas has been well-acknowledged.…”

Synthesis and in vitro and in vivo anti-inflammatory activity of novel 4-ferrocenylchroman-2-one derivatives

“…The pro-inflammatory cytokines including IL-6 and TNF-α are involved in the pathogenesis of RA. It has been reported that TNF-α and IL-6 participate in synovial proliferation and induced cartilage destruction 15 . TNF-α was particularly proven to be a key pathogenic inflammatory cytokine of RA 16 .…”

7-Hydroxycoumarin Attenuates Colistin-Induced Kidney Injury in Mice Through the Decreased Level of Histone Deacetylase 1 and the Activation of Nrf2 Signaling Pathway

“…Therefore, the development of nephroprotective agents to reduce this unwanted side-effect is urgent until more effective therapeutic options for multidrug-resistant Gram-negative bacterial infections can be found. 7-HC, a member of coumarin family, commonly found in many plants such as Eucommiae Cortex and garden angelica ( Ouyang et al, 2019 ; Wu et al, 2020 ). Recently, 7-HC has gained an increasing focus because of its extensive pharmacological properties such as anti-oxidant and anti-inflammation activities.…”