&lt;p&gt;Evaluation of Anthelminthic Activity of Tropical Taniferous Plant Extracts Against &lt;em&gt;Haemonchus contortus&lt;/em&gt;&lt;/p&gt;

Birhan, Mastewal; Gesses, Tilahun; Kenubih, Ambaye; Dejene, Haileyesus; Yayeh, Muluken

doi:10.2147/vmrr.s225717

Cited by 9 publications

(20 citation statements)

References 21 publications

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“…The tannin-rich plants constitute a non-conventional, natural alternative treatment for gastrointestinal nematodes in ruminants. Condensed tannins (CT) are a diverse group of secondary plant metabolites that have proved essential effects as ananti-Haemonchus anthelmintic; through tests of egg hatchability and larval development (29)(30)(31), while its efficacy on H. contortus adult worms is poorly studied. The present study detected procyanidin C by HPLC-ESI-MS/MS in the CT fraction.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo anthelmintic efficacy of condensed tannins extracted from the seeds of alfalfa (Medicago sativa L.) against Haemonchus contortus infection

Kandil¹,

Shalaby²,

Hendawy³

et al. 2023

IJVS

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This study was designed to examine in vitro and in vivo anthelmintic efficacy of condensed tannins (CT) extracted from seeds of Medicago sativa on Haemonchus contortus in sheep. CT's in vitro anthelmintic effect was assessed at a 300 μg/ml concentration compared with albendazole (reference drug) at 10 μg/ml. The results showed that CT had a nematocidal effect on H. contortus, and the cuticle of the adult worm appeared to be its initial target. For the in vivo experiment, nine 3-month-old helminths-free lambs were distributed into three groups. Group 1 (n=3) was challenged only as infected untreated controls; Group 2 (n=3) was treated with condensed tannin, and Group 3 (n=3) was treated with albendazole. Fecal and blood samples were collected every 3 days until the end of the experiment; for fecal egg count (FEC) and anti-H. contortus IgG titers determination, respectively. The lambs treated with the CT in G2 exhibited a pronounced decrease of mean FEC with great FECR% detected from the first-week post-treatment (PT) until the end of the experiment compared with G1 animals. The antibody levels gradually increased in G2 following the 2 nd dose of CT treatment compared to other groups. A brilliant consistent relation between the elevation of IgG response and reduction of FEC was observed following the second booster dosing of the CT in G2. In conclusion, the CT evoked strongly in vitro and in vivo anthelmintic activity against H. contortus and could be used as a natural alternative treatment of high potency against haemonchosis in sheep.

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo anthelmintic efficacy of condensed tannins extracted from the seeds of alfalfa (Medicago sativa L.) against Haemonchus contortus infection

Kandil¹,

Shalaby²,

Hendawy³

et al. 2023

IJVS

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“…Hoste et al (2009) observaron reducciones en la migración larval con concentraciones similares, pero de extractos de plantas leñosas contra larvas L3 de H. contortus, T. circumcincta y T. colubriformis de ovinos y caprinos. Birhan et al (2020) investigaron la actividad antihelmíntica in vitro de extractos de plantas taníferas tropicales sobre la inhibición del desarrollo larvario de Haemonchus contortus en ovejas y encontraron que los extractos demostraron un efecto inhibitorio dependiente de la dosis. Molan et al (2000) encontraron que extractos de Sulla (Hedysarum coronarium) adicionados al fluido ruminal y abomasal inhiben la migración de larvas L3 de H. contortus en un 72% y 81%, respectivamente, a la concentración de 1 mg/ml de TC.…”

Section: Discussionunclassified

“…Además, presentan diferencias relacionadas con tiempos y temperaturas de incubación. Varios estudios in vitro reportan que existe una relación dosis-dependiente entre la eficacia antihelmíntica y la concentración de los metabolitos secundarios de las plantas (MSP) (taninos o flavonoides) presentes en los extractos de plantas (Alonso et al 2008;Athanasiadou et al 2001;Azando et al 2011;Birhan et al 2020). Sin embargo, en nuestro estudio las concentraciones de 15 mg/ml y 30 mg/ml que fueron las más altas utilizadas y de mejor comportamiento, mostraron una tendencia a estabilizar el efecto antihelmíntico, lo que indica que probablemente existe un umbral antihelmíntico para los taninos utilizados en este ensayo.…”

Section: Discussionunclassified

“…Durante mucho tiempo el control de nematodos gastrointestinales en rumiantes se ha realizado casi exclusivamente con fármacos antihelmínticos; sin embargo, el inadecuado uso de estos medicamentos como las inexactas dosificaciones, la aplicación continua del mismo principio activo y elevada frecuencia de tratamientos condujeron al desarrollo de resistencia a la mayoría de principios químicos comúnmente disponibles (resistencia múltiple), especialmente en explotaciones ovinas y caprinas (Gasbarre 2014;Kaplan y Vidyashankar 2012;Birhan et al 2020).…”

Section: Introductionunclassified

“…La creciente aparición de resistencia de los nematodos a los antihelmínticos y la presencia de residuos de drogas en los alimentos y su acción sobre otros organismos en el ambiente han causado la emergente necesidad de encontrar alternativas para el control y reducir el uso de antihelmínticos (Learmount et al 2015;Moreno 2010;Birhan et al 2020). Entre ellas el uso de plantas bioactivas con propiedades antihelmínticas es una de las opciones más prometedoras como tratamiento fitoterapéuticos o nutracéuticos por su comprobada eficacia contra nematodos, como opción de reemplazo parcial o total del uso de químicos sintéticos corrientemente usados, y es un método amigable con el medioambiente (Mederos et al 2012;Moreno 2010).…”

Section: Introductionunclassified

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Eficacia antiparasitaria in vitro del extracto de quebracho (Schinopsis balansae) sobre larvas infectantes de Haemonchus contortus de ovinos

Vergara

Sagüés

Späth

et al. 2021

Rev. Med. Vet. Zoot.

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El objetivo fue evaluar in vitro la eficacia del extracto de quebracho (Bioquina®), rico en taninos condensados, en el control de H. contortus de ovinos, dadas las evidencias de que los taninos condensados provenientes del extracto de quebracho (Schinopsis spp.) pueden reducir la excreción de huevos, la fecundidad de las hembras parásitas y la carga de parásitos nematodos gastrointestinales adultos en rumiantes. Se utilizó el test de inhibición de migración larval (IML) a 3 concentraciones diferentes (5 mg/ml, 15 mg/ml y 30 mg/ml) sobre larvas infectantes de H. contortus susceptibles a todos los grupos químicos. El efecto se analizó mediante un análisis de varianza y para la estimación de las diferencias entre grupos (a posteriori) se usó la prueba LSD Fisher. El test in vitro demostró que el extracto de quebracho evaluado redujo aproximadamente entre un 74% y un 80% la migración larval a las concentraciones de entre 5 mg/ml y 30 mg/ml. Del análisis de varianza surgen diferencias significativas entre tratamientos (p = 0,0494). Al realizar la prueba de comparación de medias se evidenciaron diferencias significativas (p < 0,05) entre los promedios de migración a las diluciones de 5 mg/ml y 15 mg/ml y de 5 mg/ml y 30 mg/ml, mientras que no se detectaron diferencias significativas entre la dilución de 15 mg/ml y 30 mg/ml. Estos resultados señalaron que el extracto de quebracho, a las diluciones evaluadas, presentó actividad antihelmíntica sobre larvas L3 susceptibles de H. contortus; sin embargo, se requiere ampliar los estudios in vivo en ovinos.

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Nutritional Quality of Indigenous Legume Browse in the Southern Ethiopia: Farmers’ Preference and Correlation of Local Valuation of Feed Value with Scientific Indicators

Abraham¹,

Kechero²,

Andualem³

2023

Preprint

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The research was carried out in southern Ethiopia to determine farmers' preferences for indigenous legume fodder trees and shrubs (ILFTS), as well as the relationship between local feed valuation and scientific parameters. A focus group discussion (FGD) was conducted with ten farmers in each agro-ecological zone to determine the benchmarks for the preference ratings. The respondent farmers used the preference score sheet to rate all the ILFTS on an individual basis. Twenty farmers with extensive experience in ILFTS took part in the preference score ranking of each plant species in each agroecological zone. Dry matter (DM), organic matter (OM), Ash, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and metabolizable energy (ME) content of samples were determined. The standard two-stage in-vitro approach was used to measure the in vitro dry matter digestibility (IVDMD) of samples. ANOVA was used to analyze the variation among the species in agroecosystems. Farmers evaluated the ILFTS using a variety of parameters, according to the study (feed value, growth rate, biomass output, compatibility, and multifunctionality). The farmers ILFTS preference score with the evaluation criteria differed considerably (P<0.05) in agro-ecological zones. The nutritive value of ILFTS was in acceptable range for feeding ruminants though exhibited a wide variation among the species in agroecological zones. The CP content was above the minimum requirement (8%) to support the normal function of rumen microorganisms. Moreover, CP exhibited positive significant correlation with IVDMD, IVOMD and DOMD unlike the CT and ADL which exhibited negative significant correlation. Thus, ADL and CT were identified as feed fractions that inhibit IVDMD by either by depressing the activity of rumen microorganisms or restricting enzyme access to cell wall components. Conversely, the DM, OM, CP, IVDMD, IVOMD, DOMD and ME were shown a positive significant correlation with farmers feed value preference score, unlike the ADL and CT which exhibited a negative significant correlation. In conclusion, Farmers' indigenous knowledge of feed value is therefore relevant to some extent for judging the nutritive value of the ILFTS and could complement the scientific indicators.

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<p>Evaluation of Anthelminthic Activity of Tropical Taniferous Plant Extracts Against <em>Haemonchus contortus</em></p>

Cited by 9 publications

References 21 publications

In vitro and in vivo anthelmintic efficacy of condensed tannins extracted from the seeds of alfalfa (Medicago sativa L.) against Haemonchus contortus infection

In vitro and in vivo anthelmintic efficacy of condensed tannins extracted from the seeds of alfalfa (Medicago sativa L.) against Haemonchus contortus infection

Eficacia antiparasitaria in vitro del extracto de quebracho (Schinopsis balansae) sobre larvas infectantes de Haemonchus contortus de ovinos

Nutritional Quality of Indigenous Legume Browse in the Southern Ethiopia: Farmers’ Preference and Correlation of Local Valuation of Feed Value with Scientific Indicators

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