&lt;p&gt;Exosomes-Coated miR-34a Displays Potent Antitumor Activity in Pancreatic Cancer Both in vitro and in vivo&lt;/p&gt;

Zuo, Ling; Tao, Hongyu; Xu, Huanli; Liu, Cong; Qiao, Gan; Guo, Mingyue; Cao, Shousong; Liu, Minghua; Lin, Xiukun

doi:10.2147/dddt.s265423

Cited by 26 publications

(13 citation statements)

References 31 publications

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“…During TP, the segmental or diffuse inflammation of the pancreatic tissues can lead to pancreatic necrosis, fibrosis, and atrophy, and eventually lead to the disappearance of acinar cells and islet cells. This significantly damages pancreatic structure, and severely affects endocrine and exocrine function of pancreas [25,26]. Therefore, controlling acinar cell apoptosis and improving pancreatic microcirculation is expected to delay post-traumatic necrosis and promote the repair of pancreatic tissue.…”

Section: Discussionmentioning

confidence: 99%

Human umbilical cord mesenchymal stem cells-derived exosomes for treating traumatic pancreatitis in rats

et al. 2022

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Background The therapeutic and protective effects of human umbilical cord mesenchymal stem cells-exosomes (hucMSC-Exs) on traumatic pancreatitis (TP) remain unknown. Here, we established a rat model of TP and evaluated and compared the therapeutic effects of hUC-MSCs and hucMSC-Exs. Methods HucMSC-Exs were obtained by ultracentrifugation and identified using transmission electron microscopy and western blot analysis. TP rats were treated by tail vein injection of hUC-MSCs and hucMSC-Exs. Their homing in rats was observed by performing fluorescence microscopy. The degree of pancreatic tissue damage was assessed by HE staining, the expression levels of amylase, lipase, and inflammatory cytokines were detected by ELISA, apoptosis was detected by TUNEL assay, and the expression levels of various apoptosis-related proteins were detected by western-blot. The expression levels of apoptosis-related molecular markers were detected by RT-qPCR. Results The colonization of exosomes was observed in pancreatic tissue. Compared to TP group, the histopathological score of pancreas was significantly decreased in the TP + hUC-MSCs group and TP + hucMSC-Exs group (P < 0.05). Compared to TP group, the activity of serum amylase and lipase was significantly decreased (P < 0.05). The expression levels of IL-6 and TNF-α were significantly decreased, while those of IL-10 and TGF-β were significantly increased (P < 0.05). The apoptosis index of the TP group was significantly increased (P < 0.05), whereas that of the TP + hUC-MSCs and TP + hucMSC-Exs groups was significantly decreased (P < 0.05). Compared to TP group, the expression levels of Bax, Bcl-2, and Caspase-3 were significantly decreased in the TP + hUC-MSCs group and TP + hucMSC-Exs group (P < 0.05). Conclusion HucMSC-Exs can colonize injured pancreatic tissue, inhibit the apoptosis of acinar cells, and control the systemic inflammatory response to facilitate the repair of pancreatic tissue.

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Section: Discussionmentioning

confidence: 99%

Human umbilical cord mesenchymal stem cells-derived exosomes for treating traumatic pancreatitis in rats

et al. 2022

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“…Exosomes from human umbilical cord MSC could deliver exogenous miR-145-5p to specifically downregulate the expression of Smad3, thereby inhibiting the proliferation and invasion, and promoting apoptosis of PDAC cells [ 72 ]. Zuo et al [ 73 ] encapsulated the miR-34a into HEK293 cell-derived exosomes by sonication, and found that the synthesized exosomal miR-34a can be taken up by tumor cells and downregulate the expression of the target gene Bcl-2 in PDAC cells, both in vitro and in vivo. In 2016, Su et al [ 74 ] used synthetic hyaluronic acid-poly (ethylene imine) and hyaluronic acid-poly (ethylene glycol) self-assembled nanoparticles to encapsulate and deliver plasmid DNA expressing miR-155 and miR-125b into Panc-1 cells, and these miR-155 and miR-125b enriched exosomes could promote macrophage reprogramming from M2 phenotype to M1 phenotype in the TME of PDAC [ 75 ].…”

Section: The Current Application Of Tme-related Exosomal Mirnas In Th...mentioning

confidence: 99%

Crosstalk between Pancreatic Cancer Cells and Cancer-Associated Fibroblasts in the Tumor Microenvironment Mediated by Exosomal MicroRNAs

Chu

Yang

Tian

2022

IJMS

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Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant digestive tumors, characterized by a low rate of early diagnosis, strong invasiveness, and early metastasis. The abundant stromal cells, dense extracellular matrix, and lack of blood supply in PDAC limit the penetration of chemotherapeutic drugs, resulting in poor efficacy of the current treatment regimens. Cancer-associated fibroblasts (CAFs) are the major stromal cells in the tumor microenvironment. Tumor cells can secrete exosomes to promote the generation of activated CAFs, meanwhile exosomes secreted by CAFs help promote tumor progression. The aberrant expression of miRNAs in exosomes is involved in the interaction between tumor cells and CAFs, which provides the possibility for the application of exosomal miRNAs in the diagnosis and treatment of PDAC. The current article reviews the mechanism of exosomal miRNAs in the crosstalk between PDAC cells and CAFs in the tumor microenvironment, in order to improve the understanding of TME regulation and provide evidence for designing diagnostic and therapeutic targets against exosome miRNA in human PDAC.

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“…For instance, miR-126-enriched EVs can inhibit cell growth by modulating mitochondrial metabolism [191,192]. EVs can also be loaded with different miRNAs that induce cell death via the intrinsic mitochondrial pathway by downregulating Bcl-2 family antiapoptotic proteins [190,[193][194][195]. Bcl-2 downregulation can also be achieved by delivering alternative EV cargo biomolecules including silencing RNA (siRNA) and antisense oligonucleotides (ASOs) [196,197] or by depleting EVs of tumorigenic circular RNA (circRNA) [104].…”

Section: Evs Enriched With Biological Cargomentioning

confidence: 99%

Extracellular Vesicle-Mediated Mitochondrial Reprogramming in Cancer

Carles-Fontana

Heaton

Palma

et al. 2022

Cancers

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Altered metabolism is a defining hallmark of cancer. Metabolic adaptations are often linked to a reprogramming of the mitochondria due to the importance of these organelles in energy production and biosynthesis. Cancer cells present heterogeneous metabolic phenotypes that can be modulated by signals originating from the tumor microenvironment. Extracellular vesicles (EVs) are recognized as key players in intercellular communications and mediate many of the hallmarks of cancer via the delivery of their diverse biological cargo molecules. Firstly, this review introduces the most characteristic changes that the EV-biogenesis machinery and mitochondria undergo in the context of cancer. Then, it focuses on the EV-driven processes which alter mitochondrial structure, composition, and function to provide a survival advantage to cancer cells in the context of the hallmarks of cancers, such as altered metabolic strategies, migration and invasiveness, immune surveillance escape, and evasion of apoptosis. Finally, it explores the as yet untapped potential of targeting mitochondria using EVs as delivery vectors as a promising cancer therapeutic strategy.

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<p>Exosomes-Coated miR-34a Displays Potent Antitumor Activity in Pancreatic Cancer Both in vitro and in vivo</p>

Cited by 26 publications

References 31 publications

Human umbilical cord mesenchymal stem cells-derived exosomes for treating traumatic pancreatitis in rats

Human umbilical cord mesenchymal stem cells-derived exosomes for treating traumatic pancreatitis in rats

Crosstalk between Pancreatic Cancer Cells and Cancer-Associated Fibroblasts in the Tumor Microenvironment Mediated by Exosomal MicroRNAs

Extracellular Vesicle-Mediated Mitochondrial Reprogramming in Cancer

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