<p>Expression of CD133 and CD24 and their different phenotypes in urinary bladder carcinoma</p>

Abstract

Introduction: Several lines of evidence suggest the contribution of cancer stem cells (CSCs) to the tumorigenicity of bladder cancer. Although CD133 and CD24 CSC biomarkers are associated with survival disadvantages in some cancers, the biological attributes of a specific tumor alters the expression of these markers and any associated phenotypic characteristics. Aim: To analyze CD133 and CD24 expression and their different phenotypes in urinary bladder carcinoma. Material and methods: Expression of CD133 and CD24 and their divergent phenotypes were analyzed in patients with urinary bladder carcinoma (n=60) and correlated with clinicopathological parameters. Results: CD133 + and CD24 + tumor cells were more frequent in high grade, less differentiated carcinomas (18/22, and 15/17, p =0.022 and 0.01, respectively), muscle invasive tumors (20/22, p =0.017 and 17/17, p =0.001, respectively), and tumors with advanced stage ( p =0.001 and 0.007, respectively). The expression of CD24 slightly correlated with lymphovascular invasion ( p =0.04), whereas CD133 was associated with distant metastasis. The CD133 + CD24 + phenotype exhibited more aggressive tumorigenic behavior than other phenotypes. Conclusion: CD133 + and CD24 + cells correlated with determinants of aggressive behavior and may be involved in tumor progression and distant metastasis. The CD133 + subpopulation is likely to have a more potent tumorigenic capacity. Although divergent, the strong correlation between the two populations may support phenotypic plasticity among them. Compared to the CD133 + CD24 − and CD133 − CD24 + phenotypes, the CD133 + CD24 + phenotype is the most aggressive. These putative biomarkers can potentially aid in the selection of high-risk patients for more aggressive targeted therapy.

Fragile Gene WWOX Guides TFAP2A/TFAP2C-Dependent Actions Against Tumor Progression in Grade II Bladder Cancer

“…The last two are also downstream genes of canonical Wnt signaling pathway which orchestrates survival and self-renewal ( 46 , 47 ). Lastly, Cluster of Differentiation 133 ( CD133 ) downregulation by AP-2α overexpression might suggest independent mechanism focused on suppressing cancer stem cells’ growth and self-renewal ( 45 ) which can be crucial for BLCA since CD133 has been proposed as important molecule in terms of bladder cancer viability ( 48 ). Regarding the other variants, overexpression of either WWOX and AP-2α (W/A) or WWOX and AP-2γ (W/C) showed an interesting effect.…”

Expression of cancer stem cells markers in urinary bladder urothelial carcinoma and its precursor lesions

“…In recent work of Farid et al 17 , 50 cases of urothelial carcinomas including low-grade and high-grade tumors of various stages were analyzed. Similarly to our results, the expression of CD133 and CD24 was associated with increasing tumor grade and depth of the invasion.…”

Profiling and Targeting of Energy and Redox Metabolism in Grade 2 Bladder Cancer Cells with Different Invasiveness Properties

“…Compared to RT112, monolayers of 5637 cells show higher levels of some markers of stemness, and invasiveness, typically linked to tumor malignancy [ 49 , 50 ], namely a higher positivity to the fluorescent dye Aldefluor TM ( Figure 2 a), a readout of the expression of ALDH1A1 [ 51 , 52 ], and in expression level of the transmembrane glycoprotein CD44 [ 53 , 54 ] ( Figure 2 b), but not of CD133 [ 55 , 56 ] ( Figure 2 c). In agreement with previous studies [ 57 ], spheroid growth in both lines is associated with increased expression and nuclear localization (activation) of the transcription factor SOX2 ( Figure 2 d), a member of the SRY-related HMG-box (SOX) family.…”