<p>Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings</p>

Abstract

Background: In vitro and in vivo studies have shown that metallic implants coated with nano hydroxyapatite (HA) reduce the time needed for complete osseointegration compared to metallic implants coated with conventional or micron-sized HA. Moreover, due to their biologically inspired nanometer dimensions, amphiphilic peptide nanoparticles (APNPs) can also promote osteoblast attachment and enhance other cell functions if used as a coating material. Coatings made of HA and APNPs could improve osteoblast functions, but have never been tested. Purpose: The objective of this study was to prepare coatings of nanocrystalline HA and APNPs on poly(2-hydroxyethyl methacrylate) (pHEMA) coatings in order to improve osteoblast (boneforming cells) adhesion and cell density. Methods: HA was synthesized by a wet chemical process. Coatings were synthesized with different conditions and components. Results: X-ray diffraction infrared spectroscopy, transmission electron microscopy, and electron diffraction showed that nanocrystalline HA was synthesized with an expected nano size and shape distribution but with low impurities. pHEMA hydrogels with HA and APNPs increased osteoblast densities after 3 days compared to controls. Conclusion: Since cell proliferation is a prerequisite function for bone formation, these results imply that the current materials should be tested for a wide range of orthopedic applications.

Nano-hydroxyapatite coating synthesized on quasi-fibrillar superstructures of collagen hydrolysate leads to superior osteoblast proliferation when compared to nano-hydroxyapatite synthesized on collagen fibrils

“…8d provides an illustration of the cell adhesion and proliferation occurring on three different coating surfaces. The osteoblast study corroborated previous reports that nanometric HA surfaces support higher osteoblast cell density [71,72] and provided evidence that HA grown on CH was a better osteophilic material than C/HA.…”

“…HA crystals with smaller dimensions effectively influence their biological and mechanical responses when used as coating materials [71,72]. This is one of the attractive features for the utilization of CH/HA as a coating material in comparison to C/HA.…”

Biomimetic Synthesis of Nanocrystalline Hydroxyapatite Composites: Therapeutic Potential and Effects on Bone Regeneration

“…As cell proliferation is a prerequisite for bone formation, amphiphilic peptides that promote osteoblasts attachment and enhance other cell functions could improve osteoblast functions. The addition of amphiphilic peptide to nano-hydroxyapatites can induce greater osteoblast densities and then bone formation [21]. The combination of biodegradable polymers and bioactive inorganic materials is a promising method to mimic native tissue in bone tissue regeneration.…”

Novel Porous Forsterite Ceramics Biocompatibility and Bioactivity Evaluation

“…The phase purity of synthesized forsterite was reached at high temperatures by slow diffusion rates within magnesium silicate systems.Further, forsterite ceramics can even provide promising alternatives for the replacement of a bone part, [22] due to its expected high bioactivity, which is inherited from forsterite nanoparticles [14]. Furthermore, it may improve cells attachment and proliferation and, thus, it might generally reduce the necessary time for osseointegration of implanted materials, as has recently been demonstrated [23]. As such, the development of new forsterite ceramics is a continuing progress.Moreover, forsterite (Mg2SiO4) possesses good bioactivity due to its high content of Si and Mg, which can be released from forsterite, in vivo, and, are necessary in bone development.…”

“…Further, forsterite ceramics can even provide promising alternatives for the replacement of a bone part, [22] due to its expected high bioactivity, which is inherited from forsterite nanoparticles [14]. Furthermore, it may improve cells attachment and proliferation and, thus, it might generally reduce the necessary time for osseointegration of implanted materials, as has recently been demonstrated [23]. As such, the development of new forsterite ceramics is a continuing progress.…”