<p>Haptoglobin Gene Polymorphism in Patients with Sickle Cell Anemia: Findings from a Nigerian Cohort Study</p>

Abstract

Purpose: To determine the various haptoglobin genotypes and their influence on the clinicolaboratory manifestations among young Nigerian sickle cell anemia (SCA) patients. Patients and Methods: A total of 101 SCA patients and 64 controls were studied. SCA was diagnosed by polymerase chain reaction (PCR). Haptoglobin genotype was determined by PCR followed by agarose gel electrophoresis. The patients' laboratory and clinical parameters were differentiated by haptoglobin genotypes. Results: The Hp1 and Hp2 alleles frequencies were 0.62 and 0.38 in the patients and 0.73 and 0.27 in the controls, respectively, and these did not differ significantly (p>0.05). The haptoglobin genotype distribution among the patients and controls were Hp1-1, 43 (42.6%); Hp2-1, 40 (39.6%); Hp2-2, 18 (17.8%) and Hp1-1, 35 (54.7%); Hp2-1, 24 (37.5%); Hp2-2, 5 (7.8%), respectively, with no difference between the two groups (P>0.05). No significant difference was found in the clinical events and laboratory parameters of the patients when partitioned according to the various haptoglobin genotypes (P> 0.05). Conclusion: This study found that haptoglobin gene polymorphism does not have a significant influence on the clinico-laboratory manifestations among SCA patients.

Haptoglobin Gene Polymorphism among Sickle Cell Patients in West Cameroon: Hematological and Clinical Implications

“…Regarding the frequencies of VOC, these results are similar to those of Adelike and Haider [ 6 ] who observed no significant difference in the frequencies of the Hp alleles and no significant association of the Hp 2 allele with increased VOC frequency in Kuwaiti SS patients. Concerning the hematological profile, the results obtained from the present study corroborate those of Bruna et al [ 40 ], Khalid and Khalil [ 39 ], and Olatunya et al [ 38 ] who did not find any significant difference between hematological profiles regarding Hp genotypes. Nevertheless, Gueye et al [ 26 ] in Senegal found that SS patients with the Hp2-2 genotype exhibited significantly lower Hb means compared to Hp1-1 and Hp2-1 subjects.…”

“…Their research showed that the most frequent genotypes were Hp2-2 (52%) in SS patients and Hp2-1 (49%) in AA controls, and the frequency of Hp 2 allele in SS patients was 0.74. However, these are different from those of Olatunya et al [ 38 ] in Nigeria, Khalid and Khalil [ 39 ] in Sudan, Moreira and Naoum [ 24 ] in Brazil, and Ostrowski et al [ 23 ] in USA, where the most frequent Hp genotype in SS patients was Hp1-1 (43%, 68%, 36%, and 72%, respectively). Furthermore, the previous work of Bruna et al [ 40 ] in Brazil showed Hp2-1 as the most frequent Hp genotype in SS patients (57%).…”

Determinants of Care-Seeking Practices for Children with Sickle Cell Disease in Ekiti, Southwest Nigeria

“…However, the SCD patients also had earlier had their SCD status further confirmed by high-performance liquid chromatography (HPLC), Biorad, USA Variant II, using the Beta thalassemia short program and DNA Polymerase Chain Reaction (PCR). 26 …”

“…The clinical evolution record and typing of disease evolution/complications such as the occurrence of vaso-occlusive crisis (VOC), ie number of severe bone pain crises that disrupt daily activities, required hospital admission and use of opioids analgesics within the preceding 1 year prior to recruitment, as well as other details of their disease evolution/complications were as earlier described. 26 The social classes of the parents were determined according to the Oyedeji classification system using their formal educational attainment and occupation as previously described. 27…”

Influence of Haptoglobin Polymorphism on Stroke in Sickle Cell Disease Patients

“…The relationship of the Hp genotype to stroke incidence found by Barbosa et al suggests that Hp 1F-1F may be advantageous due to the correlation with lower stroke incidence [ 8 ]. In contrast, Olatunya et al reported no remarkable trends of a similar nature [ 26 ]. As previously mentioned, studies on Hp have theorized that the HP 2 allele predicts a poorer prognosis for outstanding conditions, including SCD [ 27 ].…”