&lt;p&gt;HIF-1α is a Potential Molecular Target for Herbal Medicine to Treat Diseases&lt;/p&gt;

Li, Ruo-Lan; He, Lin; Zhang, Qing; Liu, Jia; Feng, Liang; Duan, Hu-Xinyue; Fan, Linhong; Peng, Wei; Huang, Yong‐Liang; Wu, Chunjie

doi:10.2147/dddt.s274980

Cited by 19 publications

(19 citation statements)

References 174 publications

(125 reference statements)

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“…HIF-1α is a vital element for resisting oxidative stress, and is critical to proliferation, angiogenesis, energy metabolism and oxygen homeostasis (43)(44)(45). HIF-1α may also enhance aggressive phenotypes of cancer cells via various intracellular signal transduction channels (44,(46)(47)(48).…”

Section: Discussionmentioning

confidence: 99%

miR‑143‑5p suppresses breast cancer progression by targeting the HIF‑1α‑related GLUT1 pathway

Li²,

Zhang

et al. 2022

Oncol Lett

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Breast cancer (BC) is a commonly identified life-threatening type of cancer and a major cause of death among women worldwide. Several microRNAs (miRs), including miR-143-5p, have been reported to be vital for regulating hallmarks of cancer; however, the effect of miR-143-5p on BC requires further exploration. The present study performed bioinformatics analysis on GSE42072 and GSE41922 datasets from the National Center for Biotechnology Information Gene Expression Omnibus (GEO) database to identify miR-143-5p expression patterns. Furthermore, miR-143-5p expression was detected in BC cell lines and tissues via reverse transcription-quantitative PCR. Post-transfection with miR-143-5p mimics, Cell Counting Kit-8, colony formation and Transwell assays were performed to explore the effects of miR-143-5p on BC cell proliferation, colony formation, and migration. The association of miR-143-5p with the hypoxia-inducible factor-1α (HIF-1α)-associated glucose transporter 1 (GLUT1) pathway was explored via western blotting, immunofluorescence and dual-luciferase reporter assay. The present study detected high expression of miR-143-5p in BC tissue of the GSE42072 and serum of the GSE41922 datasets by GEO chip analysis. Additionally, the expression levels of miR-143-5p were decreased in BC tissues compared with those in adjacent healthy tissues, and low miR-143-5p expression was associated with a poorer prognosis and shorter survival time in patients with BC. In vitro, miR-143-5p expression levels were decreased in BC cells, and transfection with miR-143-5p mimics suppressed BC cell proliferation, colony formation, migration. Furthermore, miR-143-5p targeted the HIF-1α-related GLUT1 pathway, and inhibited HIF-1α and GLUT1 expression. Additionally, HIF-1α agonists reversed the miR-143-5p-induced inhibition during tumorigenesis. In conclusion, miR-143-5p exhibited low expression in BC tissues, and suppressed BC cell proliferation, colony formation, migration. Moreover, the antitumor effects of miR-143-5p targeted the HIF-1α-related GLUT1 pathway.

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Section: Discussionmentioning

confidence: 99%

miR‑143‑5p suppresses breast cancer progression by targeting the HIF‑1α‑related GLUT1 pathway

Li²,

Zhang

et al. 2022

Oncol Lett

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“…Numerous researches verified the key role of HIF-1α in the mediation of oxygen homeostasis within the cellular environment. A close relationship was discovered between HIF-1α level and oxygen balance: HIF-1α level remained low under the physiological situation while it was significantly elevated under the hypoxia condition[ 30 , 31 ]. Moreover, recent study revealed that the high glucose-triggered oxidative stress accelerated Aβ aggregation via the regulation of the ROS/HIF-1α mechanism in vitro , which supported a strong relationship between ROS and HIF-1α, and that the crosstalk between the two could deteriorate the Aβ production under abnormal oxidative stress condition[ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Magnesium-L-threonate exhibited a neuroprotective effect against oxidative stress damage in HT22 cells and Alzheimer’s disease mouse model

Xiong¹,

Ruan²,

Zhao³

et al. 2022

WJP

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BACKGROUND Oxidative stress results in the production of excess reactive oxygen species (ROS) and triggers hippocampal neuronal damage as well as occupies a key role in the pathological mechanisms of neurodegenerative disorders such as Alzheimer’s disease (AD). A recent study confirmed that magnesium had an inhibitory effect against oxidative stress-related malondialdehyde in vitro . However, whether Magnesium-L-threonate (MgT) is capable of suppressing oxidative stress damage in amyloid β (Aβ) 25-35 -treated HT22 cells and the AD mouse model still remains to be investigated. AIM To explore the neuroprotective effect of MgT against oxidative stress injury in vitro and in vivo , and investigate the mechanism. METHODS Aβ 25-35 -induced HT22 cells were preconditioned with MgT for 12 h. APPswe/PS1dE9 (APP/PS1) mice were orally administered with MgT daily for 3 mo. After MgT treatment, the viability of Aβ 25-35 -treated HT22 cells was determined via conducting cell counting kit-8 test and the cognition of APP/PS1 mice was measured through the Morris Water Maze. Flow cytometry experiments were applied to assess the ROS levels of HT22 cells and measure the apoptosis rate of HT22 cells or hippocampal neurons. Expression of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X (Bax), hypoxia-inducible factor (HIF)-1α, NADPH oxidase (NOX) 4, Aβ 1-42 and phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway proteins was quantified by Western blot. RESULTS In vitro data confirmed that Aβ 25–35 -induced HT22 cells had a significantly lower cell viability, higher ROS level and higher apoptosis rates compared with those of control cells (all P < 0.001). MgT prevented the Aβ 25-35 -triggered oxidative stress damage by elevating viability and decreasing ROS formation and apoptosis of HT22 cells (all P < 0.001). APP/PS1 mice exhibited worse cognitive performance and higher apoptosis rate of hippocampal neurons than wild-type (WT) mice (all P < 0.01). Meanwhile, significant higher expression of Aβ 1-42 and NOX4 proteins was detected in APP/PS1 mice than those of WT mice (both P < 0.01). MgT also ameliorated the cognitive deficit, suppressed the apoptosis of hippocampal neuron and downregulated the expression of Aβ 1-42 and NOX4 proteins in APP/PS1 mouse (all P < 0.05). Moreover, MgT intervention significantly downregulated HIF-1α and Bax, upregulated Bcl-2 and activated the PI3K/Akt pathway both in vitro and in vivo ...

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“…10-hydroxycamptothecin (10-HCPT) is an effective topoisomerase I inhibitor used in the treatment of liver cancer. The high expression of HIF-1α in liver cancer tissues is related to 10-HCPT resistance, which is considered a potential cancer target for natural products ( Li et al, 2020 ). The upregulation of HIF-1α has been confirmed to exert anti-apoptotic effects ( Tang et al, 2021 ).…”

Section: Mechanisms Of Bbr Anticancer Effectsmentioning

confidence: 99%

Apoptosis Induction, a Sharp Edge of Berberine to Exert Anti-Cancer Effects, Focus on Breast, Lung, and Liver Cancer

et al. 2022

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Cancer is the leading cause of death and one of the greatest barriers to increased life expectancy worldwide. Currently, chemotherapy with synthetic drugs remains one of the predominant ways for cancer treatment, which may lead to drug resistance and normal organ damage. Increasing researches have suggested that apoptosis, a type of programmed cell death, is a promising way for cancer therapy. Furthermore, natural products are important sources for finding new drugs with high availability, low cost and low toxicity. As a well-known isoquinoline alkaloid, accumulating evidence has revealed that berberine (BBR) exerts potential pro-apoptotic effects on multiple cancers, including breast, lung, liver, gastric, colorectal, pancreatic, and ovarian cancers. The related potential signal pathways are AMP-activated protein kinase, mitogen-activated protein kinase, and protein kinase B pathways. In this review, we provide a timely and comprehensive summary of the detailed molecular mechanisms of BBR in treating three types of cancer (breast, lung and liver cancer) by inducing apoptosis. Furthermore, we also discuss the existing challenges and strategies to improve BBR’s bioavailability. Hopefully, this review provides valuable information for the comprehension of BBR in treating three types of cancer and highlight the pro-apoptotic effects of BBR, which would be beneficial for the further development of this natural compound as an effective clinical drug for treating cancers.

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<p>HIF-1α is a Potential Molecular Target for Herbal Medicine to Treat Diseases</p>

Cited by 19 publications

References 174 publications

miR‑143‑5p suppresses breast cancer progression by targeting the HIF‑1α‑related GLUT1 pathway

miR‑143‑5p suppresses breast cancer progression by targeting the HIF‑1α‑related GLUT1 pathway

Magnesium-L-threonate exhibited a neuroprotective effect against oxidative stress damage in HT22 cells and Alzheimer’s disease mouse model

Apoptosis Induction, a Sharp Edge of Berberine to Exert Anti-Cancer Effects, Focus on Breast, Lung, and Liver Cancer

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