&lt;p&gt;High Prevalence of 16S rRNA Methyltransferase Genes in Carbapenem-Resistant &lt;em&gt;Klebsiella pneumoniae&lt;/em&gt; Clinical Isolates Associated with Bloodstream Infections in 11 Chinese Teaching Hospitals&lt;/p&gt;

Shen, Xiaofei; Liu, Li; Yu, Jingyi; Ai, Wenxiu; Cao, Xingwei; Zhan, Qing; Guo, Yinjuan; Wang, Liangxing; Yu, Fangyou

doi:10.2147/idr.s254479

Cited by 6 publications

(7 citation statements)

References 34 publications

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“…Here, we showed that the annual rate of RMTases among carbapenemase-producing Enterobacterales showed an increasing trend, from 7.5%, 10.7%, and 11.2% to 13% from 2017 to 2020. These results are in the range of those described among K. pneumoniae in Greece, but still less than the prevalence of 42.5%, 56.9%, and 93.4% reported in India, the UK, and China, respectively [8,20,39,40].…”

Section: Discussioncontrasting

confidence: 49%

“…Such a frequent association between carbapenemase-producing and RMTase-producing Enterobacterales was already reported in different parts of the world [5][6][7][8][9][10][11][12][13]21,[27][28][29][30][31][32][33][34][35][36][37]. The origin of those isolates being identified in Switzerland could be autochthonous theoretically; nevertheless, clinical information (only partially obtained and therefore not presented in that study) showed that many of them corresponded to imported cases, and particularly from Eastern European countries, from Italy, and from the Indian subcontinent.…”

Section: Discussionmentioning

confidence: 72%

“…Recent reports from different geographical areas showed that the co-production of RMTases and carbapenemases was on the rise in Enterobacterales , such as in Spain with RmtF dominating over other RMTases and mainly being identified in Klebsiella pneumoniae and Enterobacter cloacae bearing the bla OXA-48 , bla NDM-1 , and bla VIM-1 carbapenemase genes, or in Greece with RmtB and KPC being the dominating RMTase and carbapenemase enzymes, respectively [ 6 , 7 ]. Similarly, a study performed in China showed a high prevalence of RMTases among carbapenem-resistant K. pneumoniae clinical isolates associated with bloodstream infections in eleven hospitals [ 8 ]. The dominant RMTase in that collection was RmtB, while the most common carbapenemases were of KPC type.…”

Section: Introductionmentioning

confidence: 99%

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Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

et al. 2022

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Aminoglycosides (AGs) in combination with β-lactams play an important role in antimicrobial therapy in severe infections. Pan-resistance to clinically relevant AGs increasingly arises from the production of 16S rRNA methylases (RMTases) that are mostly encoded by plasmids in Gram-negative bacteria. The recent emergence and spread of isolates encoding RMTases is worrisome, considering that they often co-produce extended-spectrum β-lactamases (ESBLs) or carbapenemases. Our study aimed to retrospectively analyze and characterize the association of carbapenem- and aminoglycoside-resistant clinical isolates in Switzerland during a 3.5-year period between January 2017 and June 2020. A total of 103 pan-aminoglycoside- and carbapenem-resistant clinical isolates were recovered at the NARA (Swiss National Reference Center for Emerging Antibiotic Resistance) during the 2017–2020 period. Carbapenemase and RMTase determinants were identified by PCR and sequencing. The characterization of plasmids bearing resistance determinants was performed by a mating-out assay followed by PCR-based replicon typing (PBRT). Clonality of the isolates was investigated by multilocus sequence typing (MLST). Over the 991 Enterobacterales collected at the NARA during this period, 103 (10.4%) of them were resistant to both carbapenems and all aminoglycosides. Among these 103 isolates, 35 isolates produced NDM-like carbapenemases, followed by OXA-48-like (n = 23), KPC-like (n = 21), or no carbapenemase (n = 13), OXA-48-like and NDM-like co-production (n = 7), and VIM-like enzymes (n = 4). The RMTases ArmA, RmtB, RmtC, RmtF, RmtG, and RmtB + RmtF were identified among 51.4%, 13.6%, 4.9%, 24.3%, 1%, and 1%, respectively. Plasmid co-localization of the carbapenemase and the RMTase encoding genes was found among ca. 20% of the isolates. A high diversity was identified in terms of the nature of associations between RMTase and carbapenemase-encoding genes, of incompatibility groups of the corresponding plasmids, and of strain genetic backgrounds, highlighting heterogeneous importations rather than clonal dissemination.

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Section: Discussioncontrasting

confidence: 49%

Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

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Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

et al. 2022

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“…These cysteine residues are located near the activated methyl of cofactor. One of the cysteine residues act as a catalytic nucleophile and other play an important role in methyl transferase mechanism ( Foster et al, 2003 ; Shen et al, 2020 ). The top 10 best confirmations are shown in Table 5 along with their ZINC ID, number of interactions, interacting residues, dock score, and minimized energy.…”

Section: Resultsmentioning

confidence: 99%

Genome-Based Drug Target Identification in Human Pathogen Streptococcus gallolyticus

et al. 2021

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Streptococcus gallolysticus (Sg) is an opportunistic Gram-positive, non-motile bacterium, which causes infective endocarditis, an inflammation of the inner lining of the heart. As Sg has acquired resistance with the available antibiotics, therefore, there is a dire need to find new therapeutic targets and potent drugs to prevent and treat this disease. In the current study, an in silico approach is utilized to link genomic data of Sg species with its proteome to identify putative therapeutic targets. A total of 1,138 core proteins have been identified using pan genomic approach. Further, using subtractive proteomic analysis, a set of 18 proteins, essential for bacteria and non-homologous to host (human), is identified. Out of these 18 proteins, 12 cytoplasmic proteins were selected as potential drug targets. These selected proteins were subjected to molecular docking against drug-like compounds retrieved from ZINC database. Furthermore, the top docked compounds with lower binding energy were identified. In this work, we have identified novel drug and vaccine targets against Sg, of which some have already been reported and validated in other species. Owing to the experimental validation, we believe our methodology and result are significant contribution for drug/vaccine target identification against Sg-caused infective endocarditis.

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“…From July 2015 to December 2018, we collected 137 unique (one isolate per patient) CRKP isolates from patients with bloodstream infections (BSIs) in 11 tertiary China teaching hospitals to explore the prevalence of 16S rRNA methyltransferase genes ( 31 ). K. pneumoniae strain 1632 and 1864 were selected from them, for they are the only two isolates harboring the novel coexistence of armA , rmtB , and bla KPC .…”

Section: Methodsmentioning

confidence: 99%

Co-Occurrence of Rare ArmA-, RmtB-, and KPC-2–Encoding Multidrug-Resistant Plasmids and Hypervirulence iuc Operon in ST11-KL47 Klebsiella pneumoniae

Zhou

Guo

et al. 2022

Microbiol Spectr

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Carbapenemase-producing Klebsiella pneumoniae have become a great challenge for antimicrobial chemotherapy, while aminoglycosides can lower the mortality rate effectively in combination therapy with them. Unfortunately, we isolated two K. pneumoniae from blood sample of patients that not only exhibited high-level resistance to carbapenems and aminoglycosides but also showed the unusual co-occurrence of the rmtB , armA , and bla KPC-2 genes.

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<p>High Prevalence of 16S rRNA Methyltransferase Genes in Carbapenem-Resistant <em>Klebsiella pneumoniae</em> Clinical Isolates Associated with Bloodstream Infections in 11 Chinese Teaching Hospitals</p>

Cited by 6 publications

References 34 publications

Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

Increasing Trends of Association of 16S rRNA Methylases and Carbapenemases in Enterobacterales Clinical Isolates from Switzerland, 2017–2020

Genome-Based Drug Target Identification in Human Pathogen Streptococcus gallolyticus

Co-Occurrence of Rare ArmA-, RmtB-, and KPC-2–Encoding Multidrug-Resistant Plasmids and Hypervirulence iuc Operon in ST11-KL47 Klebsiella pneumoniae

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