<p>Hsa_circ_0084927 Regulates Cervical Cancer Advancement via Regulation of the miR-634/TPD52 Axis</p>

Abstract

Background: Cervical cancer (CC) is a common gynecological tumor that affects women's health. Circular RNA hsa_circ_0084927 (hsa_circ_0084927) has been reported to be upregulated in CC. However, the role and regulatory mechanism of hsa_circ_0084927 in CC are unclear. Methods: Expression of hsa_circ_0084927, microRNA (miR)-634, and tumor protein D52 (TPD52) mRNA in CC tissues and cells was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation, colony formation, cell cycle progression, apoptosis, migration, and invasion of CC cells were determined with cell counting kit-8 (CCK-8), plate clone, flow cytometry, or transwell assays. The levels of cyclin D1, cleavedcaspase-3 (c-caspase 3), matrix metalloproteinase (MMP)-2, MMP-9, and TPD52 protein were evaluated with Western blotting. The targeting relationship between hsa_circ_0084927 or TPD52 and miR-634 was verified via dual-luciferase reporter and/or RNA immunoprecipitation (RIP) assays. Xenograft assay was conducted to confirm the role of hsa_-circ_0084927 in vivo. Results: Hsa_circ_0084927 and TPD52 were upregulated while miR-634 was downregulated in CC tissues and cells. Hsa_circ_0084927 silencing reduced tumor growth in vivo and induced cell cycle arrest, apoptosis, and curbed proliferation, colony formation, migration, and invasion of CC cells in vitro. Hsa_circ_0084927 regulated TPD52 expression through sponging miR-634. MiR-634 inhibitor reversed hsa_circ_0084927 knockdown-mediated impact on the malignancy of CC cells. TPD52 elevation abolished the repressive influence of miR-634 mimics on the malignancy of CC cells. Conclusion: Hsa_circ_0084927 accelerated CC advancement via upregulating TPD52 via sponging miR-634, offering a new evidence to support hsa_circ_0084927 as a promising target for CC treatment.

RETRACTED ARTICLE: Knockdown of circRNA_0007534 suppresses the tumorigenesis of cervical cancer via miR-206/GREM1 axis

“…In CC, circRNA_0023404 has been proved to serve as an oncogenic regulator by affecting the miR-136/YAP axis [11] and circHIPK3 promoted the progression of CC via modulating the miR-485-3p/FGF2 axis [12]. In addition, the circ_0007364/miR-101-5p/MAT2A axis and circ_0084927/miR-634/TPD52 axis were also found in the development of CC [13,14]. Circ_0007534 is derived from DEAD box polypeptide 42 (DDX42) gene in the chr17: 61869771-61877977, and it has been identified as a carcinogene in many types of cancers [15][16][17].…”

Circ_0060551 promotes the migration and invasion of cervical cancer by upregulating TPD52

“…As shown by the result, the expression of TPD52 was greatly down‐regulated upon circ_0060551 silencing while no obvious change was observed in that of CLDN4. Moreover, as it has been reported that circ_0084927 up‐regulates TPD52 to promote the progression of cervical cancer, 18 TPD52 was determined as the downstream mRNA of circ_0060551. Then, TPD52 was detected to be significantly up‐regulated in CC cells (Figure 3J).…”

Evaluation of Diagnostic and Prognostic Value of hsa_circ_0084927 and Analysis of Associated ceRNA Network in Colorectal Cancer

“… 22 Hsa_circ_0084927 promotes the progression of CC by up-regulating TPD52 via sponge adsorption of miR-634, which provides new evidence for its use as a promising therapeutic target. 23 The significantly high expression in these tumors suggests that they may have potential cancer-promoting possibilities, which are worthy of further study. Up to now, there is very little research on hsa_circ_0084927, and more importantly, the function and mechanism of hsa_circ_0084927 have not been fully studied, and its role in tumor is unclear.…”