<p>LINC00680 Promotes the Progression of Non-Small Cell Lung Cancer and Functions as a Sponge of miR-410-3p to Enhance HMGB1 Expression</p>

Abstract

Purpose: LINC00680 was reported to be involved in various cancers through multiple mechanisms. Therefore, we intended to investigate its role in the progression of non-small cell lung cancer (NSCLC). Materials and Methods: Firstly, quantitative real-time polymerase chain reaction (qRT-PCR) was used to test LINC00680 in NSCLC tissue and cell lines. Subsequently, A549 and H1299 cells were transfected with LINC00680 overexpressing plasmids and their proliferation and colony formation and apoptosis was tested by Transwell assay and flow cytometry. In addition, xenograft tumor experiments in nude mice also affirmed. Meanwhile, we predicted that miR-410-3p, LINC00680 and high-mobility group protein box 1(HMGB1) relationship by Starbase, dual-luciferase reporter and RIP assay. Finally, the carcinogenic effects of LINC00680 were reversed by ethyl pyruvate (EP), a specific inhibitor of HMGB1. Results: LINC00680 was upregulated in NSCLC and was closely related to the malignancy and poor prognosis of NSCLC patients. LINC00680 promoted proliferation and colony formation and inhibited apoptosis of A549 and H1299 cells. In addition, overexpressing LINC00680 accelerated the growth of NSCLC cells in xenograft tumor experiments in nude mice also affirmed. Meanwhile, high-mobility group protein box 1(HMGB1) was astoundingly amplified in NSCLC and was negatively regulated by miR-410-3p. Further, HMGB1 acted as a downstream target of miR-410-3p, upregulating miR-410-3p to attenuate HMGB1, while LINC00680 strengthened the expression of HMGB1 in A549 and H1299 cells. Discussion: Thus, these results indicated that LINC00680 was cancerogenic in NSCLC by upregulating HMGB1 via sponging miR-410-3p.

LINC00680 Modulates Docetaxel Resistance in Breast Cancer via miR-320b/CDKL5 Axis

“…Several studies revealed the function of LINC00680 in cancer, including promoting malignancy in glioblastoma [19], predicting the clinical outcome and survival of patients with sarcoma [18] and promoting the progression of non-small cell cancer [22]. Our work con rmed a new role of LINC00680 in breast cancer, breast cancer cells with docetaxel resistance shown increasing expression of both LINC00680 and CDKL5 (Fig.…”

“…So the investigation of the role of lncRNAs in breast cancer could help with the understanding of chemotherapy resistance and provide the novel therapeutic strategy. LINC00680 is rstly reported as a protective biomarker and an independent prognostic indicator of softtissue sarcoma [18], then it was reported to promote the progression of non-small cell lung cancer and glioblastoma cells [19,20]. Nevertheless, the expression pattern, biological function and underlying mechanism of LINC00680 in breast cancer progression and docetaxel resistance are scarcely unresearched.…”

The role of exosomes in lung cancer metastasis and clinical applications: an updated review

“…The study reported that LINC00680 is upregulated in NSCLC and is closely associated with malignancy and the poor prognosis of NSCLC patients. LINC00680 enhanced the growth of NSCLC cells in mice, increased proliferation and colony formation, and suppressed apoptosis of H1299 and A549 cells, by functioning as a sponge of miR-410-3p to improve HMGB1 expression [ 106 ].…”

Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilities

“…Using biologically-replicated drop-out screens, 77 lncRNAs were identified as necessary for proliferation of A549 cells. These include known NSCLC lncRNAs, such as LINC00324 (42), ZFAS1 (43), MIR31HG (44), SBF2-AS1 (45), LINC00680 (46) and LINC00511 (47), that was also found in H460. In addition, lncRNAs identified in other cancer types include LUNAR1 (48), HEIH (49) and LINC00910 (50) (Figure 2e).…”