&lt;p&gt;LncRNA EMX2OS Induces Proliferation, Invasion and Sphere Formation of Ovarian Cancer Cells via Regulating the miR-654-3p/AKT3/PD-L1 Axis&lt;/p&gt;

Duan, Meng; Fang, Ming; Wang, Changhe; Wang, Hongyan; Li, Meng

doi:10.2147/cmar.s229013

Cited by 60 publications

(50 citation statements)

References 31 publications

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“…It was shown, however, to be highly expressed in hepatocellular carcinoma and inhibited the transcription of THBS1 by recruiting DNMT3b to its promoter region [ 34 ]. Previous research has revealed that the downregulation of EMX2OS in classical papillary thyroid cancer might independently predict shorter recurrence-free survival [ 35 ], while the overexpression of EMX2OS in ovarian cancer and EMX2OS/miR-654/AKT3 axis may target PD-L1 (programmed cell death protein 1) to suppress the initiation and progression of cancer [ 36 ]. Accumulating evidences have suggested that Thrombospondin-1 (THBS1) may affect tumor immunity [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Immune-Related Four-lncRNA Signature for Patients with Cervical Cancer

Zheng

Cao

Zhang

et al. 2020

BioMed Research International

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Cervical cancer (CC) is a common gynecological malignancy for which prognostic and therapeutic biomarkers are urgently needed. The signature based on immune-related lncRNAs (IRLs) of CC has never been reported. This study is aimed at establishing an IRL signature for patients with CC. A cohort of 326 CC and 21 normal tissue samples with corresponding clinical information was included in this study. Twenty-eight IRLs were collected according to the Pearson correlation analysis between the immune score and lncRNA expression ( p < 0.01 ). Four IRLs (BZRAP1-AS1, EMX2OS, ZNF667-AS1, and CTC-429P9.1) with the most significant prognostic values ( p < 0.05 ) were identified which demonstrated an ability to stratify patients into the low-risk and high-risk groups by developing a risk score model. It was observed that patients in the low-risk group showed longer overall survival (OS) than those in the high-risk group in the training set, valid set, and total set. The area under the curve (AUC) of the receiver operating characteristic curve (ROC curve) for the four-IRL signature in predicting the one-, two-, and three-year survival rates was larger than 0.65. In addition, the low-risk and high-risk groups displayed different immune statuses in GSEA. These IRLs were also significantly correlated with immune cell infiltration. Our results showed that the IRL signature had a prognostic value for CC. Meanwhile, the specific mechanisms of the four IRLs in the development of CC were ascertained preliminarily.

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Section: Discussionmentioning

confidence: 99%

Immune-Related Four-lncRNA Signature for Patients with Cervical Cancer

Zheng

Cao

Zhang

et al. 2020

BioMed Research International

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“…For example, Yang et al showed that miR-654-3p is reduced in liver cancer and hinders tumor cell growth and invasion in vitro [ 28 ]. Duan et al demonstrated that lncRNA EMX2OS encouraged the progression of ovarian cancer cells by targeting the miR-654-3p/AKT3/PD-L1 pathway [ 29 ]. Jin et al demonstrated that circRNA circHIPK3 is a prognostic biomarker in glioma development by modulating the miR-654/IGF2BP3 axis [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0079480 promotes tumor progression in acute myeloid leukemia via miR-654-3p/HDGF axis

Chen

et al. 2020

Aging

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Circular RNAs (circRNAs) are newly-discovered endogenous non-coding RNAs that have vital functions in regulating gene expression in tumorigenesis. Nonetheless, the function of circRNAs in acute myeloid leukemia (AML) are not yet clarified. In this analysis, hsa_circ_0079480, a novel circRNA, has been identified as being highly expressed in AML. Loss-of-function assays showed that reduction of hsa_circ_0079480 decreased the growth and stimulated apoptosis of AML cells in vitro . Furthermore, miR-654-3p was sponged by hsa_circ_0079480, and hepatoma-derived growth factor (HDGF) was targeted by miR-654-3p with respect to the fundamental mechanism. Moreover, the influence on growth and apoptosis of AML cells stimulated by hsa_circ_0079480 inhibition can be rescued by miR-654-3p inhibitor or HDGF overexpression. In summary, hsa_circ_0079480 is highly expressed in AML and drives by tumor progression via regulation of hsa_circ_0079480/miR-654-3p/HDGF axis, indicating that hsa_circ_0079480 may function as a new treatment target for AML therapy.

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“…miRNAs affect the malignant cell proliferation, migration and invasion capacities at post-transcriptional regulation level via binding to the 3 -UTR of the target mRNA (Yu et al, 2018;Ali Syeda et al, 2020). Reportedly, miR-654-3p targets several genes associated with malignancy, such as P21 in gastric cancer (Deng et al, 2020), AKT3 in ovarian cancer (Duan et al, 2020), and 6 | Reversal effect on proliferation and migration by upregulating miR-654-3p and SRC simultaneously. Relative expression levels of miR-654-3p and SRC in HCT116 and SW480 transfected with miR-NC + NC plasmid, miR-654-3p + NC plasmid or miR-654-3p + SRC plasmid at mRNA level (A) and protein level (B).…”

Section: Discussionmentioning

confidence: 99%

Downregulation of miR-654-3p in Colorectal Cancer Indicates Poor Prognosis and Promotes Cell Proliferation and Invasion by Targeting SRC

et al. 2020

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Background: MicroRNAs (miRNAs), such as miR-654-3p, regulate gene expression at the post-transcriptional level affecting malignant tumor behavior. However, the expression levels, function, and mechanism of miR-654-3p in colorectal cancer (CRC) are unknown. Methods: The expression levels of miR-654-3p and SRC in 103 CRC tissues and matched normal colorectal tissues were detected by a quantitative real-time polymerase chain reaction (qRT-PCR). miR-654-3p was overexpressed by RNA mimics and SRC knockdown by siRNA. Function-based experiments were carried out to detect the proliferation and migration abilities in CRC cell lines. Flow cytometry assay was performed to evaluate the effect of miR-654-3p on cell apoptosis and cycle distribution. Xenograft tumor models in nude mice were utilized to evaluate miR-654-3p functions in vivo. Dual-fluorescence reporter assay was used to verify the direct binding between miR-654-3p and SRC. Results: miR-654-3p was downregulated in CRC tissues as compared to matched normal colorectal tissues. The expression levels of miR-654-3p were closely associated with distant metastasis. In addition, elevated expression of miR-654-3p in CRC patients prolonged the overall survival. Upregulated miR-654-3p significantly suppressed the proliferation and migration capacity of CRC cells by enhancing apoptosis and promoting G0/G1 phase arrest. The direct binding between miR-654-3p and SRC was verified by the dual-luciferase reporter gene. Furthermore, the suppression of proliferation and migration capacity by elevated miR-654-3p level could be reversed by overexpressing SRC. Conclusion: miR-654-3p acts as a tumor suppressor through regulating SRC. It might also serve as a diagnostic and prognostic indicator and a novel molecular target for CRC therapy.

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<p>LncRNA EMX2OS Induces Proliferation, Invasion and Sphere Formation of Ovarian Cancer Cells via Regulating the miR-654-3p/AKT3/PD-L1 Axis</p>

Cited by 60 publications

References 31 publications

Immune-Related Four-lncRNA Signature for Patients with Cervical Cancer

Immune-Related Four-lncRNA Signature for Patients with Cervical Cancer

Hsa_circ_0079480 promotes tumor progression in acute myeloid leukemia via miR-654-3p/HDGF axis

Downregulation of miR-654-3p in Colorectal Cancer Indicates Poor Prognosis and Promotes Cell Proliferation and Invasion by Targeting SRC

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