<p>LncRNA HCG11 Suppresses Cell Proliferation and Promotes Apoptosis via Sponging miR-224-3p in Non-Small-Cell Lung Cancer Cells</p>

Abstract

Introduction Studies have found that Lnc-HCG11 is an important regulator of cancer. However, the function of Lnc-HCG11 in NSCLC is not known. Therefore, this experimental design was based on Lnc-HCG11 to explore the pathogenesis of NSCLC. Methods RT-qPCR was used to detect the expression of Lnc-HCG11 and miR-224-3p in NSCLC. The effects of Lnc-HCG11 and miR-224-3p on proliferation and apoptosis of NSCLC cells were detected by CCK-8 assay, Edu assay and Annexin V-FITC/PI assay. Target gene prediction and screening, luciferase reporter assays were used to verify downstream target genes for lnc-HCG11 and miR-224-3p. Western blotting was used to detect the protein expression of caspase-3. The tumor changes in mice were detected by in vivo. Results Lnc-HCG11 was significantly reduced in NSCLC. Lnc-HCG11 significantly inhibited cell proliferation of NSCLC cells and induced apoptosis. miR-224-3p was significantly elevated in the NSCLC cell line. Moreover, miR-224-3p significantly increased cell proliferation and inhibited apoptosis of NSCLC cells. Furthermore, Lnc-HCG11 was negatively correlated with miR-224-3p expression. Lnc-HCG11 over-expression was up-regulated the expression levels of c-caspase-3 and caspase-3. Finally, the results of in vivo animal models confirmed that Lnc-HCG11 inhibited tumor growth by modulating the miR-224-3p/c-caspase-3 axis. Conclusion Lnc-HCG11 could inhibit the progression of NSCLC by modulating the miR-224-3p/caspase-3 axis, and Lnc-HCG11 may be a potential therapeutic target for NSCLC.

Circ_0001806 Promotes the Proliferation, Migration and Invasion of NSCLC Cells Through miR-1182/NOVA2 Axis

“…1 Although surgery, radiotherapy, and chemotherapy have great progress, the 5-year survival rate of NSCLC patients is still less than 19% due to relapse and drug resistance, and the lack of sensitive and efficient early diagnostic markers. [2][3][4][5] Therefore, it is imperative to conduct an indepth research of the molecular mechanism of the occurrence and development of NSCLC, so as to provide a theoretical basis for diagnosis and therapy of NSCLC.…”

lncRNA HCG11 regulates the expression level of SP1 as a potential prognostic biomarker of BC during the polymerase II transcription process in an HCG11/miR-106a-3p/SP1 axis: integrated bioinformatics and experimental investigation

“…Additionally, there was a correlation between the expression of miR-224-3p and Lnc-HCG11. Caspase-3 and c-caspase-3 expression levels were up-regulated by the overexpression of lnc-HCG11 (37). The HCG11/miR-1276/CTNNB1 axis controlled the occurrence and progression of GC.…”

LncRNA HCG11 suppresses the progression of breast cancer through cooperating with miR-330-3p/FOXO1 axis

“…Some studies have reported that HCG11 was high-expressed in gastric cancer and can accelerate proliferation and migration in gastric cancer [21]. Other research have found that HCG11 was low-expressed in non-small-cell lung cancer and blocked cell proliferation and accelerated apoptosis in non-small-cell lung cancer [22]. Moreover, it is also proved that abnormally expressed HCG11 is closely related to poor prognosis in breast cancer [11], but the speci c mechanism remains unclear.…”