<p>LncRNA SLC7A11-AS1 Contributes to Lung Cancer Progression Through Facilitating TRAIP Expression by Inhibiting miR-4775</p>

Abstract

Long non-coding RNAs (lncRNAs) are important regulators of lung cancer. This article introduced a novel lncRNA, SLC7A11-AS1, whose effects on lung cancer development have been explored. Methods: Lung cancer tissues and normal tissues of 47 patients were collected. Bronchial epithelial cell line (BEAS-2B) and lung cancer cell lines (H520, H596, A549 and H1299) were cultured. H1299 and A549 cells were transfected with siSLC7A11-AS1 or siNC. The proliferation, migration and invasion of H1299 and A549 cells were detected by CCK-8 assay and Transwell experiment. Caspase-3 activity in H1299 and A549 cells was researched using caspase-3 activity detection kit. Dual-luciferase reporter gene assay and RNA pulldown assay were performed to explore the relationship between SLC7A11-AS1 and miR-4775. SLC7A11-AS1, miR-4775 and TRAIP mRNA expressions in tissues/cells were detected by qRT-PCR. Results: The up-regulated SLC7A11-AS1 in lung cancer patients was associated with metastasis and advanced tumor stage (P < 0.05). SLC7A11-AS1 was significantly upregulated in lung cancer cells (P < 0.05). Silencing of SLC7A11-AS1 prominently inhibited H1299 and A549 cells proliferation, migration and invasion in vitro (P < 0.05). SLC7A11-AS1 acted as a sponge to inhibit miR-4775 expression in H1299 and A549 cells. Meanwhile, TRAIP expression in H1299 and A549 cells was directly and negatively regulated by miR-4775. Inhibition of miR-4775 or overexpression of TRAIP in H1299 and A549 cells remarkably reversed the reduced proliferation, migration and invasion induced by SLC7A11-AS1 silencing (P < 0.05). Conclusion: SLC7A11-AS1 promoted lung cancer development by enhancing TRAIP expression via suppressing miR-4775.

WITHDRAWN: High SLC7A11 Expression Is Correlated With Poor Prognosis and Associated With Ferroptosis in Ovarian Cancer&nbsp;

“…Expression of SLC7A11 is deregulated in multiple cancers. Indeed, overexpression of SLC7A11 has been reported in nonsmall cell lung cancer, gastric cancer, and pancreatic Cancer and is associated with poor outcomes [23][24][25]. However, its role in ovarian cancer has not been reported.…”

TRAIP functions as an oncogene in hepatocellular carcinoma via Rb/EZH2/p21 signaling pathway

“…Knockdown of TRAIP in HCC led to cell apoptosis and G1/S phase arrest [16]. TRAIP is targeted by miR-4775 and promotes cell growth and migration in lung cancer [17]. These data may suggest the biological function of TRAIP affecting cancer progression depends on cellular content.…”

“…Current literatures reported the upstream regulation of TRAIP in cancer cells. LncRNA SLC7A11-AS1 induced TRAIP expression via sponging miR-4775 that inhibited TRAIP transcript [17]. Mutant p53 R158G increased the expression of TRAIP during the cytotoxic stress [14].…”

Identification of Ubiquitin-Related Gene-Pair Signatures for Predicting Tumor Microenvironment Infiltration and Drug Sensitivity of Lung Adenocarcinoma

“…Downregulation of SOCS3 expression was associated with the TNM stage and poor prognosis of patients with lung cancer [ 55 , 56 ]. TRAIP was a new regulator in DNA damage response and was associated with cancer development in LUAD [ 57 , 58 ]. The elevated expression of TRAIP in cancer samples promoted tumor metastasis and poor survival in patients with lung cancer [ 59 ].…”