<p>lncRNA small nucleolar RNA host gene 20 predicts poor prognosis in glioma and promotes cell proliferation by silencing P21</p>

Abstract

BackgroundIn multiple cancers, long non-coding RNA small nucleolar RNA host gene 20 (lncRNA SNHG20) is generally dysregulated. In the present study, both the biological role and clinicopathological value of lncRNA SNHG20 in glioma are explored.MethodsReal-time PCR was employed to determine lncRNA SNHG20 expression in glioma patients. The prognostic role of expression of lncRNA SNHG20 was evaluated in a retrospective cohort study. In addition, the association between lncRNA SNHG20 expression and the clinicopathological features of glioma patients, such as tumor recurrence, survival status, follow-up time, WHO grade, resection extent, tumor location, Karnofsky performance scale score, cystic change, tumor size, gender and age, was discussed. By constructing and transfecting siRNAs that targeted lncRNA SNHG20 into the glioma U87 cells, the effects of lncRNA SNHG20 on the proliferation and cell cycle of U87 cells were assessed through cell counting kit-8, colony formation and cell cycle assays, respectively. In addition, Western blot and real-time PCR measured the expression levels of P21 and CCNA1 in U87 cells after being transfected with SNHG20 siRNA.ResultsOur results suggested the high expression of lncRNA SNHG20 in human glioma tissues compared with normal brain tissues, which was related to recurrence-free survival and poor overall survival in glioma patients. According to the existing retrospective cohort study, high lncRNA SNHG20 expression was associated with tumor size, extent of resection, WHO grade, follow-up time, survival status and recurrence. Besides, knocking down the expression of lncRNA SNHG20 could inhibit the proliferation and colony formation abilities of glioma U87 cells through cell cycle arrest. Consequently, the expression of CCNA1 was inhibited, and the expression of P21 was up-regulated in U87 cells.ConclusionA high lncRNA SNHG20 expression level predicts the poor prognosis for glioma patients. Moreover, lncRNA SNHG20 can promote glioma proliferation through silencing P21 and thus lncRNA SNHG20 is an independent potential prognostic biomarker for glioma patients.

Prognostic value of long non-coding RNA SNHG20 in cancer

“…Meanwhile, 8 studies with 828 patients were included to evaluate the link of SNHG20 and histological grade. Heterogeneity difference was not observed among the studies ( I 2 = 0.0%, P = .572), [ 9 – 11 , 19 , 21 , 23 – 25 ] and the pooled OR was 2.11 (95% CI: 1.55–2.87, P < .001), indicating that high SNHG20 expression predicted higher histological grade (Fig. 2 b).…”

“…[ 22 ] Furthermore, increasing researches suggested that SNHG20 participated in the tumorigenesis via EMT signaling pathway and p21 signaling pathway. [ 17 , 28 , 29 ]…”

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

“…In ovarian cancer, SNHG20 can activate WNT/β-catenin signaling pathway, which promotes cell proliferation (190). SNHG20 supports glioma cell survival by sponging miR-4486 and up-regulating MDM2-p53 pathway (191), and silencing p21 (192). This lncRNA is also involved in vasculogenic mimicry, a process of pseudo-vascular formation in highly aggressive cancers, via enhancement of ZRANB2/SNHG20/FOX1 axis (193).…”

Prognostic and clinicopathological significance of SNHG20 in human cancers: a meta-analysis

“…These studies containing 1187 cancer patients had an accrual period between 2016 and 2019 and sample sizes varying from 32 to 144 (mean, 79). Each and every research study was performed in China; two studies referred to hepatocellular carcinoma [8,23], two studies involved osteosarcoma [10,24], two studies touched upon glioma [11,25], and the remaining nine studies related to colorectal cancer [12], gastric cancer [13], lung cancer [14], cervical cancer [15], esophageal carcinoma [16], oral carcinoma [17], nasopharyngeal carcinoma [18], ovarian cancer [19], and laryngeal carcinoma [20]. The whole subjects registered were separated into high and low SNHG20 group on the basis of the SNHG20 measurement results.…”

“…Initially, SNHG20 was discovered in hepatocellular carcinoma (HCC) and has been proved to function as an oncogene in HCC [8]. Subsequently, a growing body of research has identified that aberrant SNHG20 expression definitely interacted with prognostic outcomes and clinicopathological characteristics in patients with many kinds of malignancies, including bladder cancer [9], osteosarcoma [10], glioma [11], colorectal cancer [12], gastric cancer [13], lung cancer [14], cervical cancer [15], esophageal carcinoma [16], oral carcinoma [17], nasopharyngeal carcinoma [18], ovarian cancer [19], and laryngeal carcinoma [20]. Consistently, overexpression of SNHG20 could promote cell-cycle, proliferation, invasion, and migration of tumor cells via different mechanisms, while up-regulated SNHG20 is an unfavorable prognostic factor [7].…”