<p>Long Non-Coding RNA BLACAT1 Promotes the Tumorigenesis of Gastric Cancer by Sponging microRNA-149-5p and Targeting KIF2A</p>

Abstract

Objective: Gastric cancer (GC) is a gastrointestinal tumor. This study is aimed to explore the regulatory mechanism of long non-coding RNA BLACAT1 (BLACAT1)/microRNA-149-5p (miR-149-5p)/KIF2A cascade on GC. Methods: The expression of BLACAT1, miR-149-5p and KIF2A in GC was detected by qRT-PCR. The proliferation, migration and invasion of GC cells in vitro were analyzed by MTT, wound-healing and transwell assay, respectively. The xenograft tumor model was constructed in nude mice to confirm the inhibition effect of BLACAT1 knockdown on GC in vivo. Then, dual-luciferase reporter assay was used to detect the interactions among BLACAT1, miR-149-5p and KIF2A. Western blot assay was performed to determine the protein expression of KIF2A. Results: The expression of BLACAT1 and KIF2A was up-regulated in GC, but miR-149-5p expression was down-regulated. Silencing of BLACAT1 retarded the proliferation, migration and invasion of GC cells in vitro and the growth of tumor xenograft in vivo. Moreover, BLACAT1 acted as the molecular sponge of miR-149-5p to up-regulate KIF2A expression. At last, feedback experiments suggested that BLACAT1 accelerated the proliferation, migration and invasion of GC cells by regulating miR-149-5p/KIF2A axis. Conclusion: BLACAT1 facilitated the tumorigenesis of GC through regulating miR-149-5p/KIF2A axis, which indicated BLACAT1/miR-149-5p/KIF2A cascade may be a new therapeutic target.

Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression

“…We next demonstrated that miR-149-5p directly bound to CCDC6 or circDNA2 via dual-luciferase reporter gene assays and functional gain and loss experiments. Several studies 20 , 34 , 35 independently reported that miR-149-5p has a tumor-suppressive role in GC, which was consistent with our findings. In our study, the AUC for a single miR-149-5p expression level to predict the risk of GC occurrence reached 0.7102, indicating that miR-149-5p has potential as a biomarker for GC diagnosis.…”

Circular RNA 0000654 facilitates the growth of gastric cancer cells through absorbing microRNA-149-5p to up-regulate inhibin-beta A

“…Mechanistically, miR-149-5p expression reduction is witnessed in GC samples, but enhancement of miR-149-5p hinders the migratory and invasive properties of tumor cells [ 30 ]. An innovative article about GC also reports that miR-149-5p is abated in tumor samples, and further unravels that up-regulated miR-149-5p has a damaging effect on the biological activities of GC cells [ 31 ]. Notably, Yang Yang et al .…”

MiR-149-5p: An Important miRNA Regulated by Competing Endogenous RNAs in Diverse Human Cancers

“…Moreover, another study demonstrated that lncRNA BLACAT1 promotes the proliferation, migration and invasion of GC cells by regulating the miR-149-5p/KIF2A axis (50). CircNHSL1 can affect the expression of YWHAZ through sponging miR-149-5p, thereby regulating the progression of GC (51).…”