<p>Low Expression of APOB mRNA or Its Hypermethylation Predicts Favorable Overall Survival in Patients with Low-Grade Glioma</p>

Abstract

Background: This study was performed to explore the clinical and prognostic significance of APOB mRNA expression, DNA methylation and APOB mutation in patients with lowgrade glioma (LGG). Methods: Bioinformatic analysis was conducted using genomic, clinical and survival data from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases. Serum APOB protein levels were measured via immunoturbidimetry in 150 patients with LGG and 100 healthy controls from Hubei General Hospital. Results: There was a negative association between the levels of APOB mRNA and DNA methylation (r=−0.355, P<0.0001) in patients with LGG from the TCGA database. Additionally, LGG patients with low levels of APOB mRNA exhibited better overall survival (OS) than those with high levels of APOB mRNA (HR=0.637, P=0.0085). The survival time of LGG patients with APOB hypermethylation was markedly longer than that of patients with APOB hypomethylation (HR=0.423, P=0.0185). The prognostic significance of APOB mRNA and DNA methylation was also validated with the CGGA cohort, and a similar conclusion was reached. APOB gene mutations were observed in 3% of patients with LGG from the TCGA database, and no association was detected between APOB mutations and OS (P=0.164). Furthermore, the levels of APOB protein were much lower in patients with LGG than in normal individuals (P=0.0022), and the expression of APOB protein was markedly different among groups when stratified by histological type (P<0.0001) and histological-molecular classification (P<0.0001). Conclusion: APOB mRNA expression is negatively regulated by DNA methylation in patients with LGG. Low expression or hypermethylation of APOB might predict relatively favorable survival in patients with LGG.

<p>Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer</p>

“…APOC1 is a member of the apolipoprotein family that ACTS not only to transport lipids and stabilize the lipoprotein structure but also to regulate pathological processes including diabetes, Alzheimer's and inflammation. [11][12][13][14] In recent years, some studies have found that ApoA1, 15,16 ApoB, [17][18][19] ApoE 20,21 and other apolipoproteins 22 are abnormally expressed in tumors and are related to the prognosis of patients. Therefore, the role of apolipoprotein family in the occurrence and development of tumors has attracted more attention.…”

Analysis of multiple databases identifies crucial genes correlated with prognosis of hepatocellular carcinoma

“…APOB , belongs to the apolipoprotein family, forms sub-microscopic spherical particles, which transports dietary lipids from the intestine to the liver via the bloodstream 67 . Interestingly, multiple studies have indicated that APOB is associated with non-small cell lung cancer 68 , gallbladder cancer 69 , low-grade glioma 70 and primary small cell carcinoma of the esophagus 71 . Study by Lee et al showed that APOB inactivation is associated with poor outcome in HCC patients 72 .…”

Uncovering the Immune Cell Infiltration Landscape in Low-Grade Glioma for Aiding Immunotherapy

“…Some subjects experience indolent outcomes, while others develop into high-grade gliomas with undesirable outcomes [5]. Despite the less aggressiveness of LGG, patients usually have varied survival outcomes [6]. erefore, discovering precisely novel markers to predict patients' prognosis is of importance in current studies.…”