<p><strong>Description of <em>Stenomitos kolaenensis </em>and <em>S. hiloensis sp. nov. </em>(Leptolyngbyaceae, Cyanobacteria) with an emendation of the genus</strong></p>

Abstract

Stenomitos is a recently established cyanobacterial genus, some species of which appear to be cryptic. Here we describe two new species in this genus, Stenomitos kolaensis sp. nov. isolated from the Al-Fe humic podzols of a boreal forest near Nikel town, Murmansk region, Russia and S. hiloensis sp. nov. isolated from a basaltic seep wall on Akeola Road, Hilo, Hawaii, USA. Phylogenetic analyses were conducted on the 16S and 16S-23S ITS rRNA gene regions using Bayesian Inference, and Maximum Likelihood. Phylogenetic analysis of the 16S-23S ITS rRNA region resulted in both S. kolaensis and S. hiloensis forming separate clades from other Stenomitos lineages. Antarctic strains of Stenomitos frigidus (previously reported as “Leptolyngbya frigida”) show that species to be polyphyletic and in need of revision. The structure of the conserved ITS regions (Box-B, D1-D1ʹ, V2 and V3 helices) provided support for separation of the species, and the p-distances among aligned ITS regions further confirmed that a number of species exist within the genus. S. kolaensis and S. hiloensis can be distinguished from other described Stenomitos species (S. rutilans and S. tremulus) by their geographical distribution, habitat preference, 16S rRNA phylogeny, and differences in the secondary structure of the 16S-23S ITS region.

Trichotorquatus gen. nov. ‐ a new genus of soil cyanobacteria discovered from American drylands1

“…4b10 (Swofford 1998). For this analysis, indels were counted as a fifth base following standard phylogenetic methods for cyanobacterial ITS sequence data (Perkerson et al 2011, Osorio-Santos et al 2014, Bohunick a et al 2015, Mare s et al 2019, Becerra-Absal on et al 2020, Mesfin et al 2020, Shalygin et al 2020. A total of 10,000 bootstrap replicates were obtained for this analysis.…”

Description, taxonomy, and comparative genomics of a novelThermoleptolyngbyastrain isolated from hot springs of Ganzi, Sichuan China

“…frigidus (Fig. 2) (Shalygin et al, 2020). These strains are likely to belong to newly reported genus Stenomitos isolated from cave habitats on Kauai, Hawaii (Miscoe et al, 2016).…”

“…It has been applied for the construction of phylogenetic trees and through the analysis of secondary structures of 16S-23S ITS regions (Johansen et al, 2011). Numerous studies confirmed the integrated approach of analyzing 16S rRNA gene phylogeny and 16S-23S ITS secondary structure to be useful and robust for complex cyanobacterial taxonomy, as in the case of the species or genera within the family Leptolyngbyaceae (Komarek et al, 2014;Debnath et al, 2017;Shalygin et al, 2020).…”

Genome-wide investigation and analysis of microsatellites and compound microsatellites in Leptolyngbya species, Cyanobacteria

“…was proposed to a new genus as Enugrolinea bermudensis (Walter et al 2017), while L. frigida UCL18 appeared to be affiliated with a newly reported genus Stenomitos (Shalygin et al 2020).…”