&lt;p&gt;Lycopene Inhibits Epithelial–Mesenchymal Transition and Promotes Apoptosis in Oral Cancer via PI3K/AKT/m-TOR Signal Pathway&lt;/p&gt;

Wang, Ran; Lu, Xia; Yu, Riyue

doi:10.2147/dddt.s251614

Cited by 44 publications

(39 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CAL-27 and WSU-HN6 cells were incubated in vitro in the above groups from 12 to 72 h. The methods for dissolving and keeping were used as described previously. 8 , 10…”

Section: Methodsmentioning

confidence: 99%

“…CAL-27 and WSU-HN6 cells were incubated in vitro in the above groups from 12 to 72 h. The methods for dissolving and keeping were used as described previously. 8,10 Western Blotting Assay CAL-27 and WSU-HN6 cells were lysed with RIPA buffer containing protease cocktails (Thermo Fisher Scientific, Inc.). Protein concentrations were measured with the BCA protein assay reagent kit (Beyotime, Shanghai, China).…”

Section: Cell Lines and Culturementioning

confidence: 99%

“…7 Based on the previous findings, lycopene decreased intracellular and mitochondrial reactive oxygen species (ROS) level in OSCC cells, which can inhibit proliferation and induce apoptosis via PI3K/AKT/ mTOR Pathway. 8 Experts in increasing numbers are firmly believed that lycopene might be a promising anticancer agent in oral carcinogenesis. 9 Several different mechanisms of lycopene have been found in killing tumor cells, such as induce changes in the tumor vasculature, change the cell cycle distribution and improve gap-junction communications.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Lycopene on Oral Squamous Cell Carcinoma Cell Growth by Inhibiting IGF1 Pathway

Tao

Wang

2021

CMAR

View full text Add to dashboard Cite

Lycopene has produced robust clinical effects and shows a promising chemopreventive in the oral cancer and precancerous lesions. However, much is still unknown about its mechanisms of the carotenoid in protecting against oral squamous cell carcinoma (OSCC). Insulin-like growth factor 1 (IGF1) pathway serves as a key regulatory signal pathway in the tumor microenvironment, which may be associated with the angiogenesis, tumorigenicity, and cancer proliferation. The current study was focused on elucidating the potential pathway played for lycopene to exert its function in treating with OSCC. Materials and Methods: Firstly, we explored the dose-and time-response of CAL-27 and WSU-HN6 cells to lycopene. Both cells were incubated with various concentrations of lycopene (0.25, 0.5, 1, 2 µM). The inhibiting rate of cell proliferation was assessed using MTT assay. To observe the regulating effect of lycopene on OSCC, cell migration, apoptosis and tumor formation were detected in vitro and in vivo. The potential signaling pathways of OSCC cells treated with lycopene were analyzed by Affymetrix microarrays. And then, we investigated the changing of IGF1 signaling pathway, on the protein levels of tumor tissue after lycopene. Results: Cell proliferation was inhibited by lycopene in a dose-and time-dependent manner. The optimum inhibition efficiencies for OSCC cells were also found. Further, the results also demonstrated that pre-treatment of OSCC with lycopene drastically induced cell apoptosis suppresses cell migration and tumor growth. Mechanistically, ingenuity pathway analysis further revealed that IGF1 pathway participate in killing effects on OSCC after treatment of lycopene. Lycopene may inhibit the pathway by regulating protein expression of IGF1, IGF binding protein (BP) 1, IGFBP3, transcription factor Jun/AP-1 (JUN), and forkhead box O1 (FOXO1). Conclusion: These observations indicate that lycopene regulates OSCC cell growth by inhibiting IGF1 pathway, which may be a promising agent for the treatment of OSCC.

show abstract

“…CAL-27 and WSU-HN6 cells were incubated in vitro in the above groups from 12 to 72 h. The methods for dissolving and keeping were used as described previously. 8 , 10…”

Section: Methodsmentioning

confidence: 99%

Section: Cell Lines and Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Lycopene on Oral Squamous Cell Carcinoma Cell Growth by Inhibiting IGF1 Pathway

Tao

Wang

2021

CMAR

View full text Add to dashboard Cite

show abstract

“…Besides, lycopene upregulated tumor suppressor N-Myc downstream regulated 1 (NDGR1) activating p53/TP53-mediated caspase and apoptosis in PrE cells [ 134 ]. Furthermore, lycopene induced apoptosis of oral cancer cells by deactivating PI3K/Akt/m-TOR signaling via an increase in BAX and downregulation of p-PI3K, p-Akt, p-m-TOR, and Bcl-2 [ 135 ]. Also, lycopene promoted apoptosis of MCF-7 breast cancer cells in vitro probably through upregulation of p53 and BAX expression [ 136 ].…”

Section: The Use Of Carotenoids In Preclinical Cancer Researchmentioning

confidence: 99%

Carotenoids in Cancer Apoptosis—The Road from Bench to Bedside and Back

Koklesová

Líšková

Samec

et al. 2020

Cancers

View full text Add to dashboard Cite

An incidence and mortality of cancer are rapidly growing worldwide, especially due to heterogeneous character of the disease that is associated with irreversible impairment of cellular homeostasis and function. Targeting apoptosis, one of cancer hallmarks, represents a potent cancer treatment strategy. Carotenoids are phytochemicals represented by carotenes, xanthophylls, and derived compounds such as apocarotenoids that demonstrate a broad spectrum of anti-cancer effects involving pro-apoptotic signaling through extrinsic and intrinsic pathways. As demonstrated in preclinical oncology research, the apoptotic modulation is performed at post-genomic levels. Further, carotenoids demonstrate additive/synergistic action in combination with conventional oncostatic agents. In addition, a sensitization of tumor cells to anti-cancer conventional treatment can be achieved by carotenoids. The disadvantage of anti-cancer application of carotenoids is associated with their low solubility and, therefore, poor bioavailability. However, this deficiency can be improved by using nanotechnological approaches, solid dispersions, microemulsions or biofortification that significantly increase the anti-cancer and pro-apoptotic efficacy of carotenoids. Only limited number of studies dealing with apoptotic potential of carotenoids has been published in clinical sphere. Pro-apoptotic effects of carotenoids should be beneficial for individuals at high risk of cancer development. The article considers the utility of carotenoids in the framework of 3P medicine.

show abstract

“…Moreover, lycopene inhibited the EMT in murine CAL-27 oral cancer xenografts, as demonstrated by higher E-cadherin levels and reduced N-cadherin levels. Furthermore, lycopene inhibited the in vitro migration of CAL-27 and SCC-9 oral cancer cells [ 90 ]. The anti-metastatic effect of lycopene was also observed through the down-regulated expression of ITGA5 and ITGB1 [ 91 ], key players in ovarian cancer cell invasion and metastasis [ 92 ], MMP-9 , and EMT markers (TWIST, ZEB2, SNAI-1 and -2, FOXC2, FN1, TGFB-1 and -2, TGFBR1, and SMAD4) in OV-MZ-6 ovarian cancer cells [ 91 ].…”

Section: Carotenoids In Cancer Metastasismentioning

confidence: 99%

Carotenoids in Cancer Metastasis—Status Quo and Outlook

et al. 2020

View full text Add to dashboard Cite

Metastasis represents a major obstacle in cancer treatment and the leading cause of cancer-related deaths. Therefore, the identification of compounds targeting the multi-step and complex process of metastasis could improve outcomes in the management of cancer patients. Carotenoids are naturally occurring pigments with a plethora of biological activities. Carotenoids exert a potent anti-cancer capacity in various cancer models in vitro and in vivo, mediated by the modulation of signaling pathways involved in the migration and invasion of cancer cells and metastatic progression, including key regulators of the epithelial–mesenchymal transition and regulatory molecules, such as matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), urokinase plasminogen activator (uPA) and its receptor (uPAR), hypoxia-inducible factor-1α (HIF-1α), and others. Moreover, carotenoids modulate the expression of genes associated with cancer progression and inflammatory processes as key mediators of the complex process involved in metastasis. Nevertheless, due to the predominantly preclinical nature of the known anti-tumor effects of carotenoids, and unclear results from certain carotenoids in specific cancer types and/or specific parts of the population, a precise analysis of the anti-cancer effects of carotenoids is essential. The identification of carotenoids as effective compounds targeting the complex process of cancer progression could improve the outcomes of advanced cancer patients.

show abstract

<p>Lycopene Inhibits Epithelial–Mesenchymal Transition and Promotes Apoptosis in Oral Cancer via PI3K/AKT/m-TOR Signal Pathway</p>

Cited by 44 publications

References 42 publications

Effect of Lycopene on Oral Squamous Cell Carcinoma Cell Growth by Inhibiting IGF1 Pathway

Effect of Lycopene on Oral Squamous Cell Carcinoma Cell Growth by Inhibiting IGF1 Pathway

Carotenoids in Cancer Apoptosis—The Road from Bench to Bedside and Back

Carotenoids in Cancer Metastasis—Status Quo and Outlook

Contact Info

Product

Resources

About