<p>MicroRNA-374b-5p Functions as a Tumor Suppressor in Non-Small Cell Lung Cancer by Targeting FOXP1 and Predicts Prognosis of Cancer Patients</p>

Abstract

Background: Lung cancer remains the most frequent malignancy worldwide with increasing morbidity and mortality. This study aimed to assess the expression of microRNA-374b-5p (miR-374b-5p) in tissues and cell lines of non-small cell lung cancer (NSCLC) and to evaluate the prognostic value of miR-374b-5p as well as its biological function in tumor progression. Materials and Methods: Expression of miR-374b-5p in NSCLC patients and cells was estimated using quantitative real-time PCR. The prognostic value of miR-374b-5p was evaluated using Kaplan-Meier method and Cox regression analysis. Gain-of-function and loss-of-function cell experiments were performed to examine the effects of miR-374b-5p on NSCLC cell proliferation, migration and invasion. A luciferase activity assay was used to confirm the target gene of miR-374b-5p. Results: miR-374b-5p expression levels were decreased in tumorous tissues and cell lines compared with the normal tissues or cells (P < 0.05). The expression of miR-374b-5p was associated with the patients' tumor size, lymph node metastasis and TNM stage (all P < 0.05). Patients with low miR-374b-5p expression have a shorter survival time (log-rank P = 0.001), and the downregulated expression of miR-374b-5p was determined to be an independent prognostic indicator of NSCLC. In NSCLC cells, the overexpression of miR-374b-5p could inhibit NSCLC cell proliferation, migration and invasion and could directly target FOXP1. Conclusion: This study found that the decreased miR-374b-5p predicts poor prognosis of NSCLC, and the upregulation of miR-374b-5p can inhibit NSCLC cell proliferation, migration and invasion. The data obtained from this study provide a novel candidate prognostic biomarker and a potential therapeutic target for NSCLC.

LOXL1‐AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR‐374b‐5p/MMP14 axis

“…In this study, miR ‐ 374b ‐ 5p was downregulated in glioma, which revealed its function as a tumour suppressor. Similarly, miR ‐ 374b ‐ 5p targets FOXP1 and exerts a protective role in non–small‐cell lung cancer and ovarian cancer 47,48 . Additionally, both miR ‐ 374b ‐ 5p and miR ‐ 454 ‐ 3p exert inhibitory effects by regulating ZEB2 in bladder cancer 49 .…”

“…Similarly, miR ‐ 374b ‐ 5p targets FOXP1 and exerts a protective role in non–small‐cell lung cancer and ovarian cancer. 47 , 48 Additionally, both miR ‐ 374b ‐ 5p and miR ‐ 454 ‐ 3p exert inhibitory effects by regulating ZEB2 in bladder cancer. 49 Moreover, miR ‐ 374b targets FOXM1 , and its overexpression mediates adverse effects in cervical cancer.…”

The Regulatory Network and Potential Role of LINC00973-miRNA-mRNA ceRNA in the Progression of Non-Small-Cell Lung Cancer

“…At present, miR-374b-5p, miR-196a-5p, miR-138-5p, miR-150-5p, miR-196b-5p, miR-320a, miR-186-5p, etc. were related to the progress of NSCLC (30)(31)(32)(33)(34)(35)(36)(37)(38). For example, miR-138-5p could reduce the growth of NSCLC cells and increase the number of tumor-infiltrating dendritic cells (DCs).…”

hsa‐miR‐374b‐5p regulates expression of the gene U2AF homology motif (UHM) kinase 1

“…These results give evidence that hsa-miR-374-5p binds to the that quantified luciferase activity to validate the regulatory effect of miRNAs. 31,32 This indicated that the miRNA-mediated suppression is exerted at the mRNA level, probably by mRNA degradation, and not on the protein level through translational repression.…”