<p>Mycosynthesis, characterization, anticancer and antibacterial activity of silver nanoparticles from endophytic fungus <em>Talaromyces purpureogenus</em></p>

Abstract

Background: Biogenic silver nanoparticles (AgNPs) have wider range of biomedical applications. The present work synthesized Tp-AgNPs using mycelial extract of endophytic fungus Talaromyces purpureogenus (MEEF), characterized, and analyzed for antibacterial, anti-proliferation and cell wounding healing activities. Methods: The synthesized Tp-AgNPs were characterized by UV-visible spectrophotometer (UV-Vis), field emission transmission electron microscopy (FETEM) with energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), particle size analysis (PSA) and X-ray diffraction (XRD). Further, antibacterial activity was determined by Kirby–Bauer test and anti-proliferation activity was tested in human lung carcinoma A549 by water-soluble tetrazolium and flow cytometer assay. In addition, cell wounding healing activity was determined by scratch assay. Results: UV-Vis results displayed a strong absorption peak from 390 nm to 420 nm, which indicated the successful synthesis of Tp-AgNPs. FETEM-EDS results indicated the round and triangle shaped Tp-AgNPs with the average size of 25 nm in accordance with PSA. FTIR analysis indicated the involvement of various functional molecules from MEEF in the synthesis of Tp-AgNPs. XRD result proved nature of Tp-AgNPs as a high-quality crystal. The Tp-AgNPs significantly inhibited the growth of bacterial pathogens at the minimal inhibitory concentration of 16.12 μg.mL −1 for Gram + , and 13.98 μg.mL −1 for Gram − bacteria. Further, Tp-AgNPs (2 μg.mL −1 ) showed a strong anti-proliferation effect in A549. Interestingly, Tp-AgNPs was not cytotoxic to normal NIH3T3 cells. In addition, the NPs exhibited a strong cell wounding healing activity. Conclusion: This work biosynthesized AgNPs with strong antibacterial, anticancer and cell wound healing properties using endophytic fungus T. purpureogenus .

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

“…[ 348 ] Silver nanoparticles synthesized using the endophytic fungus Talaromyces purpureogenus are cytotoxic to the human lung carcinoma A549 cell line. [ 349 ]…”

Characterization, Antimicrobial and Antioxidant evaluation of biofabricated silver nanoparticles from endophytic Pantoea anthophila

“…12. Hu et al [44] observed similar ZOI against S. aureus, B. cereus, S. enterica, P. aeruginosa and E. coli by Tp-AgNPs synthesized from endophytic fungi Talaromyces purpureogenus. The results obtained con rms the broad spectrum activity of Pa-AgNPs against both Gram-positive and Gram-negative bacteria that were compared with antibiotic controls.…”

Mycogenic Metal Nanoparticles for the Treatment of Mycobacterioses

“…Extracellular production involves fewer steps and does not require cell disruption to release the MNPs or complex washing steps to recover and purify them [ 17 ]. Many studies have reported and listed the capacity of different fungal species to synthesize MNPs, from ubiquitous groups, to extremophiles, and ranging from yeasts, to filamentous fungi and mushrooms [ 18 , 54 , 58 , 80 , 81 , 82 , 83 , 84 , 85 , 86 ]. Despite this wide diversity, most mycogenic processes rely on filamentous fungi and follow a four-main steps method ( Figure 1 ).…”