<p>NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer</p>

Abstract

Purpose This study aimed to investigate the effect of NPTX1 on the prognosis of gastric cancer (GC), as well as the metastatic process in GC. Materials and methods The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were used to analyze the association between NPTX1 expression and prognosis in GC. Quantitative real-time polymerase chain reaction and Western blots were applied to examine the expression of NPTX1 in GC cell lines and expression of genes in downstream pathways. The role of NPTX1 on the migration, invasion, adhesion, and proliferation of GC cell lines was investigated with the transwell assay, the adhesion assay, and the MTT assay. Immunofluorescence staining was used to observe the effect of NPTX1 knockdown on the morphology of cells. Results According to the review of TCGA and GEO databases of GC, we found that the expression of NPTX1 increased in cancer tissues and high NPTX1 expression was correlated with poor overall survival, which was associated with lymph node stage in clinicopathologic parameters. Knockdown of NPTX1 attenuated the migration, invasion, and adhesion abilities of GC cells. According to gene set enrichment analysis, NPTX1 was found to be positively related to integrin and focal adhesion (FA). Additionally, NPTX1 knockdown decreased the expression of integrin α1 and integrin α7, followed by deregulation of the expression of p-Src, p-Akt, p-Erk, MMP2, and MMP7, as well as inhibiting the formation of FA complexes and decreasing the length of pseudopods in GC cells. Conclusion Our study provides strong evidence that NPTX1 plays a crucial role in promoting metastasis and acts as a prognostic indicator in GC.

LncRNA FOXC2-AS1 enhances FOXC2 mRNA stability to promote colorectal cancer progression via activation of Ca2+-FAK signal pathway

“…7a, b). As we know, focal adhesion kinase (FAK), Src, and paxillin are the principal components of the FA complex; the phosphorylation of these proteins was confirmed to be a critical process during the FA complex formation [25][26][27] . Western blot results are shown in Fig.…”

Multivariate gene expression‐based survival predictor model in esophageal adenocarcinoma

“…[51][52][53][54] Its function in EAC is still unclear and some studies have suggested it plays a role in regulating cell migration 55 and tumor metastasis. 56 ITPR1 is considered as the most prominent gene in regulating cancer cell resistance to NK-mediated lysis 57 and has been shown to be involved in the regulation of intracellular calcium signaling and the regulation of autophagy. 58 in vivo studies have previously indicated that ITPR1 targeting in cancer cells in combination with NK depletion contributed to tumor growth, indicating its role in the regulation of in vivo susceptibility of renal carcinoma cells to NK activities.…”

<p>FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments</p>

“…Adhesion assay was performed as previously described. 16 HGC-27 and MKN-7 cells transfected with FKBP10 siRNA or NC siRNA were seeded in 96-well plates, which were pre-coated with 10 µg/mL Matrigel at 37°C overnight at a density of 1 × 10 4 cells/200 µL. After incubating at 37°C for 30 minutes, wash three times with phosphate buffer saline (PBS) to remove non-adherent cells.…”

“…Western blotting was performed as previously described. 16 Cells were extracted in 1% Triton lysis buffer and quantified using the Coomassie brilliant blue method. Then, the cell lysates were separated by SDS-PAGE and electrophoretically transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Bedford, MA, USA).…”

“…RT-PCR was performed as previously described. 16 Primer sequences for FKBP10: Forward (5ʹ-TGCCTCCCTCAGA CTCTAACAC-3ʹ), Reverse (5ʹ-GAATGGGGGCTGGGAA GACTG-3ʹ).…”