&lt;p&gt;OIP5-AS1/miR-137/ZNF217 Axis Promotes Malignant Behaviors in Epithelial Ovarian Cancer&lt;/p&gt;

Guo, Linlin; Chen, Jiabao; Liu, Dong; Liu, Huan

doi:10.2147/cmar.s237726

Cited by 28 publications

(21 citation statements)

References 27 publications

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“…Bai and Li ( 39 ) stated that cell proliferation and migration were impeded, while more apoptotic cells were induced, following the silence of OIP5-AS1 in gastric cancer. Knockdown of OIP5-AS1 restrained cell viability, migration, invasion and induced apoptosis in OC cells during the current study, which agrees with the results of OIP5-AS1 in other OC research ( 16 , 17 ). The link between OIP5-AS1 and glycolysis has yet to be reported.…”

Section: Discussionsupporting

confidence: 92%

“…In addition, OIP5-AS1 knockdown restrains CCAAT/enhancer-binding protein α and TNF receptor-associated factor 4 levels to retard glioma cell growth via binding to miR-367-3p ( 15 ). OIP5-AS1 is reported to be overexpressed in OC and facilitates the malignant tumor growth and metastasis of OC by the miR-137/ZNF217 or miR-324-3p/NFIB axis ( 16 , 17 ). However, the regulation in glycolytic process of OC and other regulatory mechanisms underpinning OIP5-AS1 remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Long non‑coding RNA OIP5‑AS1 facilitates the progression of ovarian cancer via the miR‑128‑3p/CCNG1 axis

Liu

Wang

et al. 2021

Mol Med Rep

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long non-coding rna (lncrna) o-phthalaldehydeinteracting protein 5 antisense transcript 1 (oiP5-aS1) serves major roles in the progression of various types of cancer. The present study investigated its biological function in ovarian cancer (oc) and its mechanisms. The levels of oiP5-aS1, microrna-128-3p (mir-128-3p) and cyclin G1 (ccnG1) were examined by reverse transcription-quantitative Pcr. cell viability, apoptosis, migration and invasion were detected to analyze cellular progression. Glycolytic metabolism was assessed by detecting the levels of glucose consumption and lactate production. ccnG1 and hexokinase 2 protein levels were measured by western blotting. dual-luciferase reporter assay, rna immunoprecipitation and rna pull-down assays were performed to affirm the interaction between two molecules. oiP5-aS1 was found to be upregulated in oc tissues and cells. Knockdown of oiP5-aS1 suppressed cell viability, migration, invasion and glycolysis while promoting apoptosis in oc cells. oiP5-aS1 interacted with mir-128-3p and functioned as an oncogene by sequestering mir-128-3p. in addition, ccnG1 was a target gene for mir-128-3p and mir-128-3p regulated the ccnG1-induced effects on oc cells by downregulating ccnG1. oiP5-aS1 upregulated the expression of ccnG1 via targeting mir-128-3p. oiP5-aS1 knockdown also inhibited tumor growth of oc in vivo by modulating the expression of mir-128-3p and ccnG1. collectively, these data illustrated that the oncogenic role of oiP5-aS1 in oc was associated with the mir-128-3p/ccnG1 axis at least in part. oiP5-aS1 might be a probable diagnostic and therapeutic biomarker for the treatment of oc patients.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA OIP5‑AS1 facilitates the progression of ovarian cancer via the miR‑128‑3p/CCNG1 axis

Liu

Wang

et al. 2021

Mol Med Rep

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“…It suppressed apoptosis in vitro and could enhance tumor growth and metastasis in vivo in nude mice [ 157 , 158 ] ( Table 2 ). OIP5-AS1 functioned as a ceRNA competing with NFIB (Nuclear factor I B) and ZNF217 (Zinc finger protein 217) for binding miR-324-3p or miR-137, respectively [ 157 , 158 ]. An axis OIP5-AS1/miR-324-3p/NFIB was demonstrated using qRT-PCR, Western blotting, and the dual-luciferase reporter assay [ 157 ].…”

Section: Oncogenic Lncrnas As Cernas In Ovarian Cancermentioning

confidence: 99%

“…An axis OIP5-AS1/miR-324-3p/NFIB was demonstrated using qRT-PCR, Western blotting, and the dual-luciferase reporter assay [ 157 ]. The OIP5-AS1/miR-137/ZNF217 axis was validated via luciferase reporter, RNA pull-down, and RIP assays [ 158 ]. OIP5-AS1 promoted OvCa tumor growth and metastasis by upregulating either ZNF217 through binding and inhibiting of suppressor miR-137 or NFIB by dint of binding of suppressor miR-324-3p [ 157 , 158 ].…”

Section: Oncogenic Lncrnas As Cernas In Ovarian Cancermentioning

confidence: 99%

LncRNAs in Ovarian Cancer Progression, Metastasis, and Main Pathways: ceRNA and Alternative Mechanisms

Брага

Фридман

Moscovtsev

et al. 2020

IJMS

163

103

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Ovarian cancer (OvCa) develops asymptomatically until it reaches the advanced stages with metastasis, chemoresistance, and poor prognosis. Our review focuses on the analysis of regulatory long non-coding RNAs (lncRNAs) competing with protein-coding mRNAs for binding to miRNAs according to the model of competitive endogenous RNA (ceRNA) in OvCa. Analysis of publications showed that most lncRNAs acting as ceRNAs participate in OvCa progression: migration, invasion, epithelial-mesenchymal transition (EMT), and metastasis. More than 30 lncRNAs turned out to be predictors of survival and/or response to therapy in patients with OvCa. For a number of oncogenic (CCAT1, HOTAIR, NEAT1, and TUG1 among others) and some suppressive lncRNAs, several lncRNA/miRNA/mRNA axes were identified, which revealed various functions for each of them. Our review also considers examples of alternative mechanisms of actions for lncRNAs besides being ceRNAs, including binding directly to mRNA or protein, and some of them (DANCR, GAS5, MALAT1, and UCA1 among others) act by both mechanisms depending on the target protein. A systematic analysis based on the data from literature and Panther or KEGG (Kyoto Encyclopedia of Genes and Genomes) databases showed that a significant part of lncRNAs affects the key pathways involved in OvCa metastasis, EMT, and chemoresistance.

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“…Current study has shown that OIP5-AS1 was up-regulated in breast cancer which regulates the expression of ZEB2 by acting as competitive endogenous RNA (ceRNA) for miR-340-5p, and then promotes metastasis of breast cancer 27 . Similarly, OIP5-AS1 regulated ovarian cancer progression via miR-137/ZNF217 axis 28 . Knockdown of OIP5-AS1 up-regulates miR-410 to specifically block Wnt-7b/β-catenin pathway, thereby inhibiting the growth, invasion and migration of glioma cells, and promoting their apoptosis 29 .…”

Section: Introductionmentioning

confidence: 97%

SUMOylation of IGF2BP2 promotes vasculogenic mimicry of glioma via regulating OIP5-AS1/miR-495-3p axis

Li¹,

Wang²,

Yi³

et al. 2021

Int. J. Biol. Sci.

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Rationale: Glioma is the most common primary malignant tumor of human central nervous system, and its rich vascular characteristics make anti-angiogenic therapy become a therapeutic hotspot. However, the existence of glioma VM makes the anti-angiogenic therapy ineffective. SUMOylation is a post-translational modification that affects cell tumorigenicity by regulating the expression and activity of substrate proteins. Methods: The binding and modification of IGF2BP2 and SUMO1 were identified using Ni 2+ -NTA agarose bead pull-down assays, CO-IP and western blot; and in vitro SUMOylation assays combined with immunoprecipitation and immunofluorescence staining were performed to explore the detail affects and regulations of the SUMOylation on IGF2BP2. RT-PCR and western blot were used to detect the expression levels of IGF2BP2, OIP5-AS1, and miR-495-3p in glioma tissues and cell lines. CCK-8 assays, cell transwell assays, and three-dimensional cell culture methods were used for evaluating the function of IGF2BP2, OIP5-AS1, miR-495-3p, HIF1A and MMP14 in biological behaviors of glioma cells. Meantime, RIP and luciferase reporter assays were used for inquiring into the interactions among IGF2BP2, OIP5-AS1, miR-495-3p, HIF1A and MMP14. Eventually, the tumor xenografts in nude mice further as certained the effects of IGF2BP2 SUMOylation on glioma cells. Results: This study proved that IGF2BP2 mainly binds to SUMO1 and was SUMOylated at the lysine residues K497, K505 and K509 sites, which can be reduced by SENP1. SUMOylation increased IGF2BP2 protein expression and blocked its degradation through ubiquitin-proteasome pathway, thereby increasing its stability. The expressions of IGF2BP2 and OIP5-AS1 were up-regulated and the expression of miR-495-3p was down-regulated in both glioma tissues and cells. IGF2BP2 enhances the stability of OIP5-AS1, thereby increasing the binding of OIP5-AS1 to miR-495-3p, weakening the binding of miR-495-3p to the 3'UTR of HIF1A and MMP14 mRNA, and ultimately promoting the formation of VM in glioma. Conclusions: This study first revealed that SUMOylation of IGF2BP2 regulated OIP5-AS1/miR-495-3p axis to promote VM formation in glioma cells and xenografts growth in nude mice, providing a new idea for molecular targeted therapy of glioma.

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<p>OIP5-AS1/miR-137/ZNF217 Axis Promotes Malignant Behaviors in Epithelial Ovarian Cancer</p>

Cited by 28 publications

References 27 publications

Long non‑coding RNA OIP5‑AS1 facilitates the progression of ovarian cancer via the miR‑128‑3p/CCNG1 axis

Long non‑coding RNA OIP5‑AS1 facilitates the progression of ovarian cancer via the miR‑128‑3p/CCNG1 axis

LncRNAs in Ovarian Cancer Progression, Metastasis, and Main Pathways: ceRNA and Alternative Mechanisms

SUMOylation of IGF2BP2 promotes vasculogenic mimicry of glioma via regulating OIP5-AS1/miR-495-3p axis

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