<p>Potential role of circulating microRNAs (486-5p, 497, 509-5p and 605) in metabolic syndrome Egyptian male patients</p>

Abstract

Objective: This study aims to evaluate the expression pattern of circulating microRNAs (miR)-486-5p, miR-497, miR-509-5p, and miR-605 in the serum of metabolic syndrome (MetS) Egyptian male patients. Methods: In this study, the circulating miR-486-5p, miR-497, miR509-5p, and miR-605 were amplified and quantitatively detected by quantitative real-time polymerase chain reaction in sera of 55 MetS male patients in comparison to 20 male controls. The level of fasting plasma glucose and triacylglycerol (TAG) were measured using calorimetric assay. Blood pressure was measured using mercuric sphygmomanometer. Anthropometric measurements were done to each individual. Furthermore, MetS patients were defined according to the criteria proposed by the American Heart Association and divided into three groups according to MetS index. Results: The study was performed on three groups and a control group defined as follows: group 1: 15 MetS patients who fulfilled all diagnostic criteria of MetS; group 2: 20 MetS patients with normal blood pressure; group 3: 20 MetS patients with normal TAG levels.The levels of miRs are expressed as [median (IQR)]. miR-486-5-p and miR-497 expression were elevated in group 1 [31.9(49), p˂ 0.0001; 73.1(42.5), p˂ 0.0001], group 2 [36.4(15.7), p˂ 0.0001; 68.3(54.8), p˂ 0.0001], and group (3) [10.8(18.9), p= 0.0014; 27.5(39.7), p= 0.0012]. MiR-509-5p was elevated in groups 1 and 2 [501(468), p= 0.0001], [309(436), p= 0.0006], respectively, while normally expressed in group 3 [0.93(0.077), p= 0.0001]. miR-605 was elevated in groups 1 and 3 [25.4(20.0), p= 0.0018], [54.8(65.8), p˂ 0.0001], while normally expressed in group 2 [0.84(0.67), p˂ 0.0001]. Conclusion: miRs (486-5p, 497, 509-5p, and 605) serum levels were higher in MetS patients than in healthy control subjects; therefore, these serum miRs can serve as early biomarkers and can be used to follow-up the prognosis of MetS.

In silico transcriptional analysis of mRNA and miRNA reveals unique biosignatures that characterizes different types of diabetes

“…These findings suggest that miR-200c is a possible candidate to mediate DM complications, or at least those related to heart and vasculature. Previously, the miR-497 has been proposed to be a predictor of DM due to its upregulation during the acquisition of insulin resistance [50]. Our findings corroborate with this hypothesis by demonstrating that miR-497 expression is associated with pre-DM in the MOFA.…”

Skeletal muscle-specific overexpression of miR-486 limits mammary tumor-induced skeletal muscle functional limitations

“…Elevated circulating miR-486 levels were found in children with obesity 55 , 56 and patients with metabolic syndrome. 57 These changes in circulating miR-486 levels are significantly associated with the percentage of body fat mass and clinical measures related to obesity. 58 High glucose increases miR-486 expression that regulates adipogenic differentiation in human adipose tissue-derived mesenchymal stem cells.…”

Circular RNA MELK Promotes Chondrocyte Apoptosis and Inhibits Autophagy in Osteoarthritis by Regulating MYD88/NF‐κB Signaling Axis through MicroRNA‐497‐5p

“…Aberrant expression of miR-497-5p is mainly seen in different types of cancer [41], diabetic nephropathy [42], Parkinson's disease [43], preeclampsia [44], and metabolic syndrome [45]. In particular, Zhao et al have found that miR-497-5p can promote osteoblast differentiation in osteoporosis [46].…”