&lt;p&gt;Proteomic Applications in Antimicrobial Resistance and Clinical Microbiology Studies&lt;/p&gt;

Khodadadi, Ehsaneh; Zeinalzadeh, Elham; Taghizadeh, Sepehr; Mehramouz, Bahareh; Kamounah, Fadhil S.; Khodadadi, Ehsan; Ganbarov, Khudaverdi; Yousefi, Bahman; Bastami, Milad; Kafil, Hossein Samadi

doi:10.2147/idr.s238446

Cited by 26 publications

(15 citation statements)

References 213 publications

(226 reference statements)

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“…Transcriptomic and proteomic analysis has been widely used in studying the molecular mechanisms of the action of antibacterial agents (Khodadadi et al, 2020;Tsakou et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)

Sherif

Talat

Alaidaroos

et al. 2023

Annals of Animal Science

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The antibacterial activity of zinc oxide nanoparticles (ZnO NPs) has received significant attention worldwide due to the emergence of multidrug-resistant microorganisms. Shiga toxin-producing Escherichia coli is a major foodborne pathogen that causes gastroenteritis that may be complicated by hemorrhagic colitis or hemolytic uremic syndrome. Therefore, this study aimed to evaluate the antimicrobial effect of ZnO NPs against E. coli O26 and its Shiga toxin type 2 (Stx2). Multidrug resistance phenotype was observed in E. coli O26, with co-resistance to several unrelated families of antimicrobial agents. Different concentrations of ZnO NPs nanoparticles (20 nm) were tested against different cell densities of E. coli O26 (108, 106 and 105 CFU/ml). The minimum inhibitory concentration (MIC) value was 1 mg/ml. Minimum bactericidal concentration (MBC) was 1.5 mg/ml, 2.5 mg/ml and 3 mg/ml, respectively, depending on ZnO NPs concentrations and bacterial cell density. Results showed a significant (P≤0.05) decrease in Stx2 level in a response to ZnO NPs treatment. As detected by quantitative real-time PCR, ZnO NPs down-regulated the expression of the Stx2 gene (P≤0.05). Moreover, various concentrations of ZnO NPs considerably reduced the total protein content in E. coli O26. There was a significant reduction in protein expression with increased ZnO NPs concentration compared to the non-treated control. Scanning electron micrographs (SEM) of the treated bacteria showed severe disruptive effects on E. coli O26 with increasing ZnO NPs concentration. The results revealed a strong correlation between the antibacterial effect and ZnO NPs concentrations. ZnO NPs exert their antibacterial activities through various mechanisms and could be used as a potent antibacterial agent against E. coli O26.

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“…Transcriptomic and proteomic analysis has been widely used in studying the molecular mechanisms of the action of antibacterial agents (Khodadadi et al, 2020;Tsakou et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)

Sherif

Talat

Alaidaroos

et al. 2023

Annals of Animal Science

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“…Even though an antibiotic resistance gene marker is detected in the genotypic profile, there is no guarantee that the gene is translated and transcribed to produce the enzyme or protein to confer resistance, unless seen in the phenotypic susceptibility results. In the future, it would be helpful to incorporate a proteomics workflow into the pipeline to see which proteins are being expressed to help infer phenotypic antibiotic susceptibility profiles [ 187 ].…”

Section: Future Of Ngs In Clinical Microbiologymentioning

confidence: 99%

Next Generation and Other Sequencing Technologies in Diagnostic Microbiology and Infectious Diseases

Hilt

Ferrieri

2022

Genes

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Next-generation sequencing (NGS) technologies have become increasingly available for use in the clinical microbiology diagnostic environment. There are three main applications of these technologies in the clinical microbiology laboratory: whole genome sequencing (WGS), targeted metagenomics sequencing and shotgun metagenomics sequencing. These applications are being utilized for initial identification of pathogenic organisms, the detection of antimicrobial resistance mechanisms and for epidemiologic tracking of organisms within and outside hospital systems. In this review, we analyze these three applications and provide a comprehensive summary of how these applications are currently being used in public health, basic research, and clinical microbiology laboratory environments. In the public health arena, WGS is being used to identify and epidemiologically track food borne outbreaks and disease surveillance. In clinical hospital systems, WGS is used to identify multi-drug-resistant nosocomial infections and track the transmission of these organisms. In addition, we examine how metagenomics sequencing approaches (targeted and shotgun) are being used to circumvent the traditional and biased microbiology culture methods to identify potential pathogens directly from specimens. We also expand on the important factors to consider when implementing these technologies, and what is possible for these technologies in infectious disease diagnosis in the next 5 years.

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“…These approaches can synchronize the discovery of several pathogens in a solitary essay. They can then inhibit misdiagnosis and delays in treatment without rising costs or adding a new action to the procedure [ 98 ].…”

Section: Advantages and Disadvantages Of Maldi-tof Msmentioning

confidence: 99%

MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology

Alizadeh

Yousefi

Pakdel

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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There is a range of proteomics methods to spot and analyze bacterial protein contents such as liquid chromatography-mass spectrometry (LC-MS), two-dimensional gel electrophoresis, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS), which give comprehensive information about the microorganisms that may be helpful within the diagnosis and coverings of infections. Microorganism identification by mass spectrometry is predicted on identifying a characteristic spectrum of every species so matched with an outsized database within the instrument. MALDI-TOF MS is one of the diagnostic methods, which is a straightforward, quick, and precise technique, and is employed in microbial diagnostic laboratories these days and may replace other diagnostic methods. This method identifies various microorganisms such as bacteria, fungi, parasites, and viruses, which supply comprehensive information. One of the MALDI-TOF MS’s crucial applications is bacteriology, which helps identify bacterial species, identify toxins, and study bacterial antibiotic resistance. By knowing these cases, we will act more effectively against bacterial infections.

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<p>Proteomic Applications in Antimicrobial Resistance and Clinical Microbiology Studies</p>

Cited by 26 publications

References 213 publications

Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)

Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)

Next Generation and Other Sequencing Technologies in Diagnostic Microbiology and Infectious Diseases

MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology

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