MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology

Alizadeh, Mohammad; Yousefi, Leila; Pakdel, Farzaneh; Ghotaslou, Reza; Rezaee, Mohammad Ahangarzadeh; Khodadadi, Ehsaneh; Oskouei, Mahin Ahangar; Barhaghi, Mohammad Hossein Soroush; Kafil, Hossein Samadi

doi:10.1155/2021/9928238

Cited by 22 publications

(15 citation statements)

References 110 publications

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“…Studies have described the failure to identify strains due to the absence of a broad protein spectrum from a reference bank or even the capture of the technique of specific proteins necessary to define the species. (Bizzini et al, 2011;Alizadeh et al, 2021). However, data on its applicability reveal MALDI-TOF MS's importance in quickly typing subspecies of medical importance; the technique contributes to the proper management of antimicrobials and best hospital practices.…”

Section: Discussionmentioning

confidence: 99%

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

et al. 2023

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Bacteria responsible for causing infections are common in hospital environments, water, soil, and food products. The infection risk is intensified by the absence of public sanitation, poor quality of life, and food scarcity. These external factors promote the dissemination of pathogens by direct contamination or biofilm formation. In this work, we identified bacterial isolates obtained from intensive care units in the southern region of Tocantins, Brazil. We compared matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques and 16S ribosomal ribonucleic acid (rRNA) molecular analysis; we also performed phenotypic characterization. Fifty-six isolates characterized using morphotinctorial tests were classified as gram-positive (80.4%; n = 45) and gram-negative (19.6%; n = 11) and were resistant to several antibiotic classes; notably, we identified the blaOXA-23 resistance gene in the ILH10 isolate. Microbial identification using MALDI-TOF MS resulted in the identification of Sphingomonas paucimobilis and Bacillus circulans. 16S rRNA sequencing revealed four isolates belonging to the genera Bacillus and Acinetobacter. The similarity was superior to 99% for Acinetobacter schindleri in the Basic Local Alignment Search Tool (BLAST), grouped in the clade superior to 90%. Several strains isolated from intensive care units (ICU) were resistant to various antibiotic classes. These techniques allowed for the identification of several microorganisms of importance in public health, enabling improvements in human infection control and proving the quality of inputs, food, and water.

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Section: Discussionmentioning

confidence: 99%

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

et al. 2023

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“…To identify the cause of infectious diseases through bioaerosol, serological techniques (such as antibody-based enzyme immunoassays) and molecular amplification methods (such as PCR) are generally used because they are more specific, more sensitive, and can be performed faster, although they still require several hours [54]. MALDI TOF MS is being studied as a potential means of detecting bioaerosols.…”

Section: Maldi Tof Ms Analysis For Detection Of Bioaerosolsmentioning

confidence: 99%

Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS

2021

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MALDI TOF MS-based microbial identification significantly lowers the operational costs because of minimal requirements of substrates and reagents for extraction. Therefore, it has been widely used in varied applications such as clinical, food, military, and ecological research. However, the MALDI TOF MS method is laced with many challenges including its limitation of the reference spectrum. This review briefly introduces the background of MALDI TOF MS technology, including sample preparation and workflow. We have primarily discussed the application of MALDI TOF MS in the identification of microorganisms. Furthermore, we have discussed the current trends for bioaerosol detection using MALDI TOF MS and the limitations and challenges involved, and finally the approaches to overcome these challenges.

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“…Matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) can be utilized for the identification and characterization of microorganisms. , Analysis of MALDI-TOF MS can be used to describe numerous small biomolecules associated with specific microorganisms. Many of these biomolecules play important roles in cellular processes such as cell-to-cell communication, growth, and antibiotics. − Specific ions can be fragmented using MALDI-TOF MS coupled with collision induced dissociation (CID).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Small Biomolecule Ionization and Fragmentation in Pseudomonas aeruginosa Using Common MALDI Matrices

Wamer

Morse

Gadient

et al. 2023

J. Am. Soc. Mass Spectrom.

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Different bacterial cell surface associated biomolecules can be analyzed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and coupled with collision induced dissociation (CID) for identification. Pseudomonas aeruginosa is an opportunistic, Gram-negative bacterium that causes acute or chronic biofilm infections. Cells of P. aeruginosa communicate through a system of signaling biomolecules known as quorum sensing (QS). The QS system can result in the production of biosurfactant rhamnolipids known to associate and alter the cellular membrane. MALDI-TOF utilizes a variety of matrices that can interact differently with biomolecules for selective ionization. We examined six common matrices to determine the optimal matrix specific to different molecule classes in P. aeruginosa associated with cell surfaces. Three major molecule classes (quinolones, rhamnolipids, and phospholipids) were observed to ionize selectively with the different matrices tested. Sodiated and protonated adducts differed between matrices utilized in our study. Isobaric ions were identified as different molecule classes depending on the matrix used. We highlight the role of matrix selection in MALDI-TOF identification of molecules within a complex biological mixture.

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MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology

Cited by 22 publications

References 110 publications

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

Resistance determinants of emerging pathogens isolated from an intensive care unit as a parameter of population health conditions of the Legal Amazon microregion

Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS

Comparison of Small Biomolecule Ionization and Fragmentation in Pseudomonas aeruginosa Using Common MALDI Matrices

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