<p>Rat Bone Marrow-Derived Mesenchymal Stem Cells Promote the Migration and Invasion of Colorectal Cancer Stem Cells</p>

Abstract

Background: Colorectal cancer is one of the most common cancers and the second leading cause of cancer-related deaths worldwide. Targeting cancer stem cells (CSCs) may be a novel strategy for the treatment of colorectal cancer. Previous studies have shown that bone marrowderived MSCs (BM-MSCs) promote tumor growth and metastasis. However, the role of rat BM-MSCs in the biological behaviors of colorectal CSCs remains unclear until now. Materials and Methods: BM-MSCs were isolated from rats and characterized. CSCs were enriched from HCT116 cells using the microsphere culture method, and the microspheres incubated for at least 10 passages were termed HCT116-CSCs that were characterized. The effects of rat BM-MSCs on migration and invasion of HCT116-CSCs were examined using transwell migration and invasion assays and xenograft tumor growth assay. Results: Rat BM-MSCs appeared typical stem cell morphology. Flow cytometry revealed positive CD29 and CD44 expression in rat BM-MSCs at passage 3, and rat BM-MSCs were found to differentiate into osteocytes following incubation in osteogenic induction medium. Microscopy, flow cytometric detection of stem cell surface markers, colony-formation assay and transwell migration and invasion assays characterized the successful preparation of HCT116-CSCs, and subcutaneous injection of HCT116-CSCs produced xenograft tumors in nude mice, while HE staining of the xenograft tumors displayed cancer specimen shapes. Transwell migration and invasion assays showed that rat BM-MSCs promoted the migration and invasion of HCT116-CSCs, and injection of rat BM-MSCs was found to promote the growth of the mouse xenograft tumor derived from HCT116-CSCs. Conclusion: Rat BM-MSCs promote the migration and invasion of colorectal CSCs, and colorectal CSCs may be a potential target for the therapy against colorectal cancer.

The Effects of Mesenchymal Stem Cell on Colorectal Cancer

“…Similarly, it is reported that BM-MSCs are implanted in nude mice after subcutaneous injection of HCT116-cancer stem cells (CSCs) to construct xenograft tumors. BM-MSCs can promote the migration and invasion of CSCs in CRC, suggesting that it can be a potential therapeutic target for CRC [148]. Further studies by De Boeck and colleagues found that BM-MSCs stimulate the invasion, survival, and tumorigenesis of CRC cells by releasing soluble NRG1 and activating HER2/HER3-dependent PI3K/Akt signaling cascade in CRC cells, and the high expression of NRG1 is associated with poor prognosis [149].…”

“…Interacting with CRC cells through CCL3/4/5 -CCR5 to promote the growth of CRC tumors in vivo [148] Cell-to-cell contact Promoting the EMT process of CRC through the CCL5/β-catenin/Slug pathway and SPARC [147,151] Cell-to-cell contact…”

The Roles of Mesenchymal Stem Cells in Gastrointestinal Cancers

“…Through IL-6/JAK2/STAT3 signal transduction, human CRC MSCs could increase the migration and invasion of CRC (83). Of note, rat BMSCs were found to promote the migration and invasion of CRC (84). Oh et al demonstrated that after co-culturing MSCs with CRC cells, the TGF-b1 and p53 in MSCs increased, which enhanced the invasion and proliferation of CRC (85).…”

“…Through IL-6/JAK2/STAT3 signal transduction, human CRC MSCs could increase the migration and invasion of CRC ( 83 ). Of note, rat BMSCs were found to promote the migration and invasion of CRC ( 84 ). Oh et al.…”

Mature rat hepatocyte dedifferentiation into long lived proliferating hepatic progenitor cells

“…CD29 широко экспрессирован на различных типах клеток, включая мезенхимные и эпителиальные клетки, а также стволовые/прогениторные клетки различного происхождения [16]. Так, CD29 наряду с CD44 и CD90 зачастую рассматривается в качестве маркера мезенхимных стволовых/стромальных клеток (МСК), выделяемых из разных тканевых источников, как у человека [17], так и у грызунов [18]. Во взрослой печени интегрин β1 экспрессируют зрелые гепатоциты [19], а также печеночные стволовые клетки и гепатобласты [20].…”