&lt;p&gt;Silencing circZFR inhibits the proliferation, migration and invasion of human renal carcinoma cells by regulating miR-206&lt;/p&gt;

Wang, Mi; Gao, Yue; Liu, Jie

doi:10.2147/ott.s215012

Cited by 20 publications

(12 citation statements)

References 41 publications

(45 reference statements)

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“…Moreover, hsa_circ_001783 and circABCB10 were reported as oncogenic regulators in BC through functioning as specific miRNA sponges [ 10 , 11 ]. As for circRNA zinc finger RNA binding protein (circZFR, hsa_circ_0072088), it has been identified as an oncogenic modulator in many human cancers, such as renal carcinoma, bladder cancer and non-small cell lung cancer [ 12 – 14 ]. Nevertheless, the biological roles of circZFR in BC tumorigenesis remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

CircZFR functions as a sponge of miR-578 to promote breast cancer progression by regulating HIF1A expression

et al. 2020

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Background: Breast cancer (BC) is the most common malignancy among women. Emerging studies have demonstrated that circular RNA (circRNA) zinc finger RNA binding protein (circZFR) serves as a crucial regulator in many human cancers. However, the role and mechanism of circZFR in BC tumorigenesis remain unclear. Methods: The levels of circZFR, miR-578 and hypoxia-inducible factor 1α (HIF1A) were detected by quantitative realtime polymerase chain reaction (qRT-PCR) or western blot. Cell viability, colony formation, apoptosis, migration and invasion capacities in vitro were determined by using the Cell Counting Kit-8 (CCK-8), standard colony formation, flow cytometry and transwell assays, respectively. Glucose uptake, lactate product and adenosine triphosphate (ATP) levels of cells in vitro were measured using the commercial human assay kits. Targeted relationships among circZFR, miR-578 and HIF1A in BC cell lines were verified by dual-luciferase reporter and RNA pulldown assays. Animal studies were performed to assess the effect of circZFR on tumor growth in vivo. Results: Our data indicated that circZFR was overexpressed in BC tissues and cells, and the increased circZFR level predicted poor prognosis of BC patients. CircZFR silencing or miR-578 overexpression repressed BC cell viability, colony formation, migration, invasion, and glycolysis and enhanced cell apoptosis in vitro. CircZFR silencing also hampered tumor growth in vivo. Mechanistically, circZFR acted as a sponge of miR-578, and circZFR silencing hindered BC cell malignant behaviors by miR-578. HIF1A was a functional target of miR-578 in regulating BC cell viability, colony formation, migration, invasion, glycolysis and apoptosis in vitro. Furthermore, circZFR modulated HIF1A expression through sponging miR-578. Conclusion: Our findings first identified that the silencing of circZFR suppressed BC malignant progression in vitro via the regulation of the miR-578/HIF1A axis, providing evidence for the crucial involvement of circZFR in BC pathogenesis.

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Section: Introductionmentioning

confidence: 99%

CircZFR functions as a sponge of miR-578 to promote breast cancer progression by regulating HIF1A expression

et al. 2020

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“…Tan et al reported that circ_0072083 contributed to the progression of hepatocellular cancer via miR-3619-5p/CTNNB1 signaling [10]. Wang et al demonstrated that circ_0072083 accelerated the proliferation and metastasis of human renal carcinoma via sponging miR-206 [11]. Zhang et al found that circ_0072083 acted as an oncogene in NSCLC via miR-101-3p/CUL4B axis [12].…”

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confidence: 99%

Circ_0072083 interference enhances growth-inhibiting effects of cisplatin in non-small-cell lung cancer cells via miR-545-3p/CBLL1 axis

Liu

Qin

2020

Cancer Cell Int

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Background: Non-small-cell lung cancer (NSCLC) is one of the common cancers in the world. Circular RNA 0072083 (circ_0072083, circZFR) has been reported to be associated with the progression of NSCLC. In this study, we intended to explore the role and the potential mechanism of circ_0072083 in NSCLC. Methods: Quantitative real time polymerase chain reaction (qRT-PCR) was performed to detect the expression of circ_0072083, its matching linear RNA (zinc finger RNA binding protein (ZFR)) and microRNA-545-3p (miR-545-3p) in NSCLC cells. The ability of colony formation in NSCLC cells was detected by colony formation assay. The apoptosis and cell cycle were measured by flow cytometry. The metastasis was determined by transwell migration and invasion assays. The protein expression of E-cadherin, N-cadherin, Vimentin and Cbl proto-oncogene like 1 (CBLL1) was examined by western blot assay. The interaction between miR-545-3p and circ_0072083 or CBLL1 was predicted by starBase or Targetscan software. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were applied to validate these interactions. Nude mice bearing tumors were used to confirm the role of circ_0072083 and cisplatin (DDP) in vivo. Results: The level of circ_0072083 was higher in NSCLC tissues and cells relative to that in adjacent non-tumor tissues and normal lung cells. The transfection of si-circ_0072083 inhibited colony formation, cell cycle and metastasis while promoted the apoptosis of NSCLC cells stimulated by DDP. MiR-545-3p was a direct functional target of circ_0072083 in NSCLC cells. CBLL1 could bind to miR-545-3p in NSCLC cells. Circ_0072083 promoted the progression of NSCLC induced by DDP through sponging miR-545-3p and enhancing the enrichment of CBLL1 in vivo and in vitro. Conclusion: Circ_0072083 depletion contributed to DDP-triggered inhibition of NSCLC tumor through miR-545-3p/ CBLL1 axis.

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“…CircZFR is highly expressed in tumor tissues including nonsmall lung cell cancer (NSCLC) (11), gastric cancer (23), liver cancer (9, 10), colorectal cancer (8), renal carcinoma (12) and thyroid Cancer (7). CircZFR is also highly expressed in 104 pairs of BCa tissues (24).…”

Section: Discussionmentioning

confidence: 99%

“…CircRNA-specific computation and high-throughput RNA sequencing are main tools for circRNA research (6). Using these methods, the high expression of circZFR was found in thyroid cancer (7), rectal cancer (8), liver cancer (9,10), lung cancer (11), kidney cancer (12), where circZFRs affect the occurrence and development of tumors through regulating the cell proliferation, invasion and migration.…”

Section: Introductionmentioning

confidence: 99%

Circ-ZFR Promotes Progression of Bladder Cancer by Upregulating WNT5A Via Sponging miR-545 and miR-1270

Luo

Miao²,

Yao

et al. 2021

Front. Oncol.

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BackgroundBladder cancer is one of the most common cancers all over the world. CircZFR is a circular RNA and has been implicated in tumor generation and invasion. However, the exact role of circZFR in the development of bladder cancer (BCa) remains unknown. This study aimed to investigate the function of circZFR in BCa, and further to probe into the association between circ-ZFR, miR-545/miR-1270 and WNT5A.MethodsThe expression of circZFR in BCa was quantified by qRT-PCR and was positively correlated with the prognosis of BCa patients. Next, the stable knockdown of circZFR BCa cell lines was established and the resulting capacities of proliferation, migration and invasion were measured. The association of circZFR with miR-1270/miR-545 was predicted by circinteractome prediction, and was confirmed by luciferase assay as well as RNA pull down assay. Furthermore, miRNA inhibitors, WNT5A overexpression and Pearson correlation analysis were used to examine the relationship between circZFR, miR-1270/miR-545 and WNT5A.ResultsThe expression of CircZFR was up-regulated both in BCa tissues and in BCa cell lines, and was positively correlated with patient survival rates. Blocking of circZFR’s expression by RNA inhibitors suppressed the proliferation, migration and invasion of BCa cells both in vitro and in vivo. On the other hand, overexpression of target miRNA supported that circZFR directly interact with miR-545 and miR-1270. Moreover, we demonstrated that circZFR promotes the progression of BCa by upregulating WNT5A’s expression via sponging miR-545 and miR-1270.ConclusionsCircZFR promotes the proliferation, migration and invasion of BCa cells by upregulating WNT5A signaling pathway via sponging miR-545 and miR-1270. These results provide new insights into the molecular mechanism of circZFR in BCa progression, and more important, a novel target for BCa clinical treatment.

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<p>Silencing circZFR inhibits the proliferation, migration and invasion of human renal carcinoma cells by regulating miR-206</p>

Cited by 20 publications

References 41 publications

CircZFR functions as a sponge of miR-578 to promote breast cancer progression by regulating HIF1A expression

CircZFR functions as a sponge of miR-578 to promote breast cancer progression by regulating HIF1A expression

Circ_0072083 interference enhances growth-inhibiting effects of cisplatin in non-small-cell lung cancer cells via miR-545-3p/CBLL1 axis

Circ-ZFR Promotes Progression of Bladder Cancer by Upregulating WNT5A Via Sponging miR-545 and miR-1270

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