<p>Targeting ZEB2 By microRNA-129 In Non-Small Cell Lung Cancer Suppresses Cell Proliferation, Invasion And Migration Via Regulating Wnt/β-Catenin Signaling Pathway And Epithelial–Mesenchymal Transition</p>

Abstract

IntroductionNon-small cell lung cancer (NSCLC) is a common cause of deaths all over the world. Emerging evidence has indicated that microRNA (miR) play key roles in NSCLC progression. We aimed to determine the functions of miR-129 in NSCLC. miR-129 was dramatically downregulated in NSCLC tissue samples and cells. The decreased miR-129 was found to be associated with poorer prognosis and malefic phenotype of NSCLC patients. We demonstrated that miR-129 upregulation could inhibit NSCLC cell growth. Furthermore, we also sought the molecular mechanism by which miR-129 repressed NSCLC development.MethodsQRT-PCR was applied to detect the expressions of miR-129 in 51 pairs of NSCLC tissue samples. We further performed the Kaplan–Meier analysis to determine the association between miR-129 expressions and the survival rate of NSCLC patients. We then measured the expression levels of miR-129 in NSCLC cell lines. After that, MTT assays were performed to determine the influence of miR-129 on A549 cell proliferation. Transwell assay was then conducted to explore the biological functions of miR-129 in invasion and migration of NSCLC cells.ResultsResults showed that ZEB2 was directly targeted by miR-129 in NSCLC cell lines. Moreover, miR-129 restoration could inhibit EMT and Wnt/β-catenin in NSCLC cell lines.ConclusionIn short, all these results indicated that miR-129/ZEB2 axis maybe a useful diagnostic and prognostic biomarker for NSCLC treatment.

<p>MicroRNA-374b-5p Functions as a Tumor Suppressor in Non-Small Cell Lung Cancer by Targeting FOXP1 and Predicts Prognosis of Cancer Patients</p>

“…28 Li et al reported that the overexpression of miR-129 in NSCLC cells could inhibit cell proliferation, invasion and migration by directly regulating ZEB2, and might be a useful biomarker and therapeutic target of NSCLC. 29 To further uncover the functional role of miR-374b-5p in NSCLC progression, we regulated its expression in NSCLC cells and checked its effects on cell biological function. The overexpression of miR-374b-5p was found to inhibit NSCLC cell proliferation, migration and invasion, while the reduction of miR-374b-5p led to the opposite results, indicating that miR-374b-5p might serve as a tumor suppressive miRNA and a potential therapeutic target of NSCLC.…”

Role of microRNA‑129 in cancer and non‑cancerous diseases (Review)

“…Zinc finger E-box binding homeobox 2 (ZEB2) is a member of the ZEB family of transcription factors and is known to help mediate EMT (36). In non-small cell lung cancer (NSCLC), miR-129 was found to directly target ZEB2 to inhibit NSCLC cell proliferation (37). miR-129 also regulates the Wnt/β-catenin signaling pathway by decreasing the expression of activated β-catenin, c-Myc and cyclin D1.…”

Targeting the miR-6734-3p/ZEB2 axis hampers development of non-small cell lung cancer (NSCLC) and increases susceptibility of cancer cells to cisplatin treatment

“…Given that ZEB2 and miR-6734-3p played opposite regulating role in NSCLC progression [ 23 , 35 ], and there existed regulating relationship between these two genes, we explored whether miR-6374-3p regulated NSCLC development through targeting ZEB2. To validate this hypothesis, we determined the effects of miR-6734-3p/ZEB2 axis on the malignant phenotypes in NSCLC.…”